LOCUS LT727166 7683 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pBK-1, complete sequence.
ACCESSION LT727166
VERSION LT727166.1
KEYWORDS .
SOURCE Vector pBK-1
ORGANISM Vector pBK-1
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7683)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..7683
/organism="Vector pBK-1"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4707."
/db_xref="taxon:1945254"
CDS <1..809
/codon_start=3
/transl_table=11
/note="part of SV40 VP1"
/protein_id="SJL87641.1"
/translation="PLPNLNEDLTCGNLLMWEAVTVQTEVIGITSMLNLHAGSQKVHE
HGGGKPIQGSNFHFFAVGGDPLEMQGVLMNYRTKYPDGTITPKNPTAQSQVMNTDHKA
YLDKNNAYPVECWVPDPSRNENTRYFGTFTGGENVPPVLHVTNTATTVLLDEQGVGPL
CKADSLYVSAADICGLFTNSSGTQQWRGLARYFKIRLRKRSVKNPYLISFLLSDLINR
RTQRVDGQPMYGMESQVEEVRVFDGTERLPGDPDMIRYIDKQGQLQTKML"
regulatory complement(810..878)
/note="polyA"
/regulatory_class="terminator"
regulatory 810..878
/note="polyA"
/regulatory_class="terminator"
CDS complement(879..2723)
/transl_table=11
/note="part of SV40 large t-antigen"
/protein_id="SJL87642.1"
/translation="MPTYGTEEWESWWSSFNEKWDEDLFCHEDMFASDEEATADSQHS
TPPKKKRKVEDPKDFPSDLHQFLSQAVFSNRTLACFAVYTTKEKAQILYKKLMEKYSV
TFISRHMCAGHNIIFFLTPHRHRVSAINNFCQKLCTFSFLICKGVNKEYLLYSALTRD
PYHTIEESIQGGLKEHDFNPEEPEETKQVSWKLITEYAVETKCEDVFLLLGMYLEFQY
NVEECKKCQKKDQPYHFKYHEKHFANAIIFAESKNKKVICQQAVDTVLAKKRVDTLHM
TREEMLTERFNHILDKMDLIFGAHGNAVLEQYMAGVAWLHCLLPKMDSVIFDFLHCIV
FNVPKRRYWLFKGPIDSGKTTLAAGLLDLCRGKALNVNLPMERLTFELGVAIDQYMVV
FEDVKGTGAESKDLPSGHGINNLDSLRDYLDGSVKVNLEKKHLNKRTQIFPPGLVTMN
EYPVPKTLQARFVRQIDFRPKIYLRKSLQNSEFLLEKRILQSGMTLLLLLIWFRPVAD
FATDIQSRIVEWKERLDSEISMYTFSRMKYNICMGKCILDITREEDSETEDSGHGSST
ESQSQCSSQVSDTSAPAEDSQRSDPHSQELHLCKGFQCFKRPKTPPPK"
misc_feature complement(2724..3067)
/note="intron of SV40 large t-antigen"
CDS complement(2792..3310)
/transl_table=11
/note="SV40 small t-antigen"
/protein_id="SJL87643.1"
/translation="MDKVLNREESMELMDLLGLERAAWGNLPLMRKAYLRKCKEFHPD
KGGDEDKMKRMNTLYKKMEQDVKVAHQPDFGTWSSSEVCADFPLCPDTLYCKEWPICS
KKPSVHCPCMLCQLRLRHLNRKFLRKEPLVWIDCYCIDCFTQWFGLDLTEETLQWWVQ
IIGETPFRDLKL"
CDS complement(<3068..3310)
/transl_table=11
/note="part of SV40 large t-antigen"
/protein_id="SJL87644.1"
/translation="MDKVLNREESMELMDLLGLERAAWGNLPLMRKAYLRKCKEFHPD
KGGDEDKMKRMNTLYKKMEQDVKVAHQPDFGTWSSSE"
regulatory complement(3405..3768)
/note="BKVE"
/regulatory_class="promoter"
regulatory 3405..3768
/note="BKVL"
/regulatory_class="promoter"
rep_origin complement(3407..3429)
/note="BK ori"
CDS 3977..5032
/transl_table=11
/note="VP2"
/protein_id="SJL87645.1"
/translation="MGAALALLGDLVASVSEAAAATGFSVAEIAAGEAAAAIEVQIAS
LATVEGITSTSEAIAAIGLTPQTYAVIAGAPGAIAGFAALIQTVSGISSLAQVGYRFF
SDWDHKVSTVGLYQQSGMALELFNPDEYYDILFPGVNTFVNNIQYLDPRHWGPSLFAT
ISQALWHVIRDDIPSITSQELQRRTERFFRDSLARFLEETTWTIVNAPINFYNYIQQY
YSDLSPIRPSMVRQVAEREGTRVHFGHTYSIDDADSIEEVTQRMDLRNQQSVHSGEFI
EKTIAPGGANQRTAPQWMLPLLLGLYGTVTPALEACEDGPNQKKRRVSRGSSQKAKGT
RASAKTTNKRRSRSSRS"
misc_feature 5090..7683
/note="pML"
rep_origin complement(5806..6074)
/note="pMB1 ori"
CDS complement(6615..7475)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87646.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 2111 a 1709 c 1666 g 2197 t
ORIGIN
1 ttcccctccc caatttaaat gaggacctaa cctgtggaaa tctactgatg tgggaggctg
61 taactgtaca aacagaggtt attggaataa ctagcatgct taaccttcat gcagggtcac
121 aaaaagtgca tgagcatggt ggaggaaaac ctattcaagg cagtaatttc cacttctttg
181 ctgttggtgg agaccccttg gaaatgcagg gagtgctaat gaattacagg acaaagtacc
241 cagatggtac tataacccct aaaaacccaa cagcccagtc ccaggtaatg aatactgacc
301 ataaggccta tttggacaaa aacaatgctt atccagttga gtgctgggtt cctgatccta
361 gtagaaatga aaatactagg tattttggga ctttcacagg aggggaaaat gttcccccag
421 tacttcatgt gaccaacaca gctaccacag tgttgctaga tgaacagggt gtggggcctc
481 tttgtaaagc tgatagcctg tatgtttcag ctgctgatat ttgtggcctg tttactaaca
541 gctctggaac acaacagtgg agaggccttg caagatattt taagatccgc ctgagaaaaa
601 gatctgtaaa gaatccttac ctaatttcct ttttgctaag tgaccttata aacaggagaa
661 cccagagagt ggatgggcag cctatgtatg gtatggaatc ccaggtagaa gaggttaggg
721 tgtttgatgg cacagaaaga cttccagggg acccagatat gataagatat attgacaaac
781 agggacaatt gcaaaccaaa atgctttaaa caggtgcttt tattgtacat atacatttaa
841 taaatgctgc ttttgtataa gccactttta agcttgtgtt attttggggg tggtgtttta
901 ggccttttaa aacactgaaa gcctttacac aaatgcaact cttgactatg ggggtctgac
961 ctttgggaat cttcagcagg ggctgaagta tctgagactt gggaagagca ttgtgattgg
1021 gattcagtgc ttgatccatg tccagagtct tcagtttctg aatcctcttc tcttgtaata
1081 tcaagaatac atttccccat gcatatatta tatttcatcc ttgaaaaagt atacatactt
1141 atctcagaat ccagcctttc cttccattca acaattctag attgtatatc agttgcaaaa
1201 tcagctacag gcctaaacca aattagcagt agcaacaagg tcattccact ttgtaaaatt
1261 cttttttcaa gtaagaactc tgagttttgt aaggattttc ttaaatatat tttgggccta
1321 aaatctattt gtcttacaaa tctagcttgc agggttttag ggacaggata ctcattcatt
1381 gtaaccaagc ctggtggaaa tatttgggtt cttttgttta aatgtttctt ttctaaattt
1441 accttaacac ttccatctaa ataatctctc aaactgtcta aattgtttat tccatgtcct
1501 gaaggcaaat cctttgattc agctcctgtc ccttttacat cttcaaaaac aaccatgtac
1561 tgatctatag ctacacctag ctcaaaggtt agcctttcca tgggtaggtt tacatttaag
1621 gctttacctc tacacaaatc taacaaccct gcagctagtg ttgtttttcc actatcaatg
1681 ggacctttaa ataaccagta tcttctttta ggtacattga aaacaataca gtgcaaaaaa
1741 tcaaatatta cagaatccat tttaggtagc aaacagtgca gccaagcaac acctgccata
1801 tattgttcta gtacagcatt tccatgagct ccaaatatta aatccatttt atctaatata
1861 tgattgaatc tttctgttag catttcttcc ctggtcatat gaagggtatc tactcttttt
1921 ttagctaaaa ctgtatctac tgcttgctga caaataactt ttttgttttt actttctgca
1981 aaaataatag catttgcaaa gtgcttttca tgatacttaa agtgataagg ctggtctttt
2041 ttctgacact ttttacactc ctctacattg tattgaaatt ctaaatacat acctaataat
2101 aaaaacacat cctcacactt tgtctctact gcatactcag taattaattt ccaagacacc
2161 tgctttgttt cttcaggctc ttctgggtta aaatcatgct cctttaagcc cccttgaatg
2221 ctttcttcta tagtatggta tggatctcta gttaaggcac tatatagtaa gtattcctta
2281 ttaacaccct tacaaattaa aaaactaaag gtacacagct tttgacagaa attattaatt
2341 gcagaaactc tatgtctatg tggagttaaa aagaatataa tattatgccc agcacacatg
2401 tgtctactaa taaaagttac agaatatttt tccataagtt ttttatacag aatttgagct
2461 ttttctttag tagtatacac agcaaagcag gcaagggttc tattactaaa tacagcttga
2521 ctaagaaact ggtgtagatc agagggaaag tctttagggt cttctacctt tctttttttt
2581 ttgggtggtg ttgagtgttg agaatctgct gttgcttctt catcactggc aaacatatct
2641 tcatggcaaa ataaatcttc atcccatttt tcattaaagg aactccacca ggactcccac
2701 tcttctgttc cataggttgg cacctataaa aaaaataatt acttagggcc ttttaatatt
2761 ttattattta tctaaatata agttagttac cttaaagctt tagatctctg aagggagttt
2821 ctccaattat ttggacccac cattgcagag tttcttcagt taggtctaag ccaaaccact
2881 gtgtgaagca gtcaatgcag tagcaatcta tccaaaccaa gggctctttt cttaaaaatt
2941 ttctatttaa atgccttaat ctaagctgac atagcatgca agggcagtgc acagaaggct
3001 ttttggaaca aataggccat tccttgcagt acagggtatc tgggcaaaga ggaaaatcag
3061 cacaaacctc tgagctactc caggttccaa aatcaggctg atgagctacc tttacatcct
3121 gctccatttt tttatacaaa gtattcattc tcttcatttt atcctcgtcg ccccctttgt
3181 cagggtgaaa ttccttacac ttccttaaat aagcttttct cattaaggga agatttcccc
3241 aggcagctct ttcaaggcct aaaaggtcca tgagctccat ggattcttcc ctgttaagaa
3301 ctttatccat ttttgcaaaa attgcaaaag aatagggatt tccccaaata gttttgctag
3361 gcctcagaaa aagcctccac acccttacta cttgagagaa agggtggagg cagaggcggc
3421 ctcggcctct tatatattat aaaaaaaaag gccacaggga ggagctgctt acccatggaa
3481 tgcagccaaa ccatgacctc aggaaggaaa gtgcatgact cacaggggaa tgcagccaaa
3541 ccatgacctc aggaaggaaa gtgcatgact cacagggagg agctgcttac ccatggaatg
3601 cagccaaacc atgacctcag gaaggaaagt gcatgactgg gcagccagcc agtggcagtt
3661 aatagtgaaa ccccgccgac agacatgttt tgcgagccta ggaatcttgg ccttgtcccc
3721 agttaaactg gacaaaggcc atggttctgc gccagctgtc acgacaagct tcagtgaaag
3781 ttggtaaaac ctggactgga acaaaaaaaa gagctcagag gatttttatt tttattttag
3841 agcttttgct ggaattttgt agaggtgaag acagtgtaga cgggaaaaac aaaagtacca
3901 ctgctttacc tgctgtaaaa gactctgtaa aagactccta ggtaagtaat cccttttttt
3961 ttgtatttcc aggttcatgg gtgctgctct agcacttttg ggggacctag ttgccagtgt
4021 atctgaggct gctgctgcca caggattttc agtggctgaa attgctgctg gggaggctgc
4081 tgctgctata gaagttcaaa ttgcatccct tgctactgta gagggcataa caagtacctc
4141 agaggctata gctgctatag gcctaactcc tcaaacatat gctgtaattg ctggtgctcc
4201 tggggctatt gctgggtttg ctgctttaat tcaaactgtt agtggtatta gttccttggc
4261 tcaagtaggg tataggttct ttagtgattg ggatcacaaa gtttccactg taggcctcta
4321 tcagcaatca ggcatggctt tggaattgtt taacccagat gagtactatg atattctgtt
4381 tcctggtgta aatacttttg ttaataatat tcaatacctt gatcctaggc attggggtcc
4441 ttctttgttt gctactattt cccaggcttt gtggcatgtt attagggatg atataccttc
4501 tataacctca caggaattgc agagaagaac agaaagattt tttagagact ccttggctag
4561 atttttggag gaaactacct ggacaattgt aaatgcccct ataaactttt ataattatat
4621 tcaacaatat tattctgatc tttcccctat taggccctca atggttagac aagtagctga
4681 aagggaaggt acccgtgtac attttggcca tacttatagt atagatgatg ctgacagtat
4741 agaagaagtt acacaaagaa tggacttaag aaatcaacaa agtgtacatt caggagagtt
4801 tatagaaaaa actattgccc caggaggtgc taatcaaaga actgctcctc aatggatgtt
4861 gcctttactt ctaggcctgt acgggactgt aacacctgct cttgaagcat gtgaagatgg
4921 ccccaaccaa aagaaaagga gagtgtccag gggcagctcc caaaaagcca aaggaacccg
4981 tgcaagtgcc aaaactacta ataaaaggag gagtagaagt tctagaagtt aaaactgggg
5041 tagatgctat tacagaggta gaatgcttcc taaacccaga aatgggggat cctctacgcc
5101 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
5161 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
5221 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca
5281 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
5341 caggagtcgc ataagggaga gcgtcgaccg atgcccttga gagccttcaa cccagtcagc
5401 tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc
5461 atgcaactcg taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt
5521 cgctggagcg cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc
5581 gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc
5641 gccggcatgg cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg
5701 atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag
5761 gccatgctgt ccaggcaggt agatgacgac catcagggac agcttaaagg ccagcaaaag
5821 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
5881 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
5941 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
6001 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
6061 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
6121 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
6181 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
6241 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
6301 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
6361 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
6421 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
6481 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
6541 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
6601 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
6661 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
6721 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
6781 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
6841 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
6901 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt
6961 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
7021 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
7081 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
7141 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
7201 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga
7261 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
7321 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
7381 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
7441 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
7501 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
7561 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
7621 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa
7681 gaa
//