LOCUS LT727165 7087 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian vector pdcDNA-FLAGMyc, complete sequence. ACCESSION LT727165 VERSION LT727165.1 KEYWORDS . SOURCE Mammalian vector pdcDNA-FLAGMyc ORGANISM Mammalian vector pdcDNA-FLAGMyc other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 7087) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..7087 /organism="Mammalian vector pdcDNA-FLAGMyc" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 4705." /db_xref="taxon:1945171" regulatory 27..610 /note="hCMV-IE promoter and enhancer" /regulatory_class="promoter" regulatory 655..671 /note="T7 promoter" /regulatory_class="promoter" misc_feature 696..719 /note="FLAG epitope" regulatory complement(729..853) /note="attR1" /regulatory_class="attenuator" regulatory 861..913 /note="lacUV5 promoter" /regulatory_class="promoter" CDS 962..1621 /transl_table=11 /note="chloramphenicol resistance" /protein_id="SJL87637.1" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A" CDS 1963..2268 /transl_table=11 /note="ccdB" /protein_id="SJL87638.1" /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" regulatory 2309..2433 /note="attR2" /regulatory_class="attenuator" misc_feature 2438..2467 /note="Myc epitope" regulatory complement(2506..2522) /note="SP6 promoter" /regulatory_class="promoter" regulatory 2548..2772 /note="BGH polyA" /regulatory_class="terminator" rep_origin 2818..3246 /note="f1 ori" regulatory 3251..3460 /note="SV40 early promoter" /regulatory_class="promoter" rep_origin 3417..3492 /note="SV40 ori" CDS 3584..4378 /transl_table=11 /note="Tn5 neomycin resistance" /protein_id="SJL87639.1" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" regulatory 4552..4626 /note="SV40 polyA early" /regulatory_class="terminator" regulatory complement(4552..4626) /note="SV40 polyA late" /regulatory_class="terminator" regulatory complement(4765..4848) /note="lac promoter" /regulatory_class="promoter" rep_origin complement(4940..5342) /note="pMB1 ori" CDS complement(5883..6743) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL87640.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" BASE COUNT 1729 a 1743 c 1772 g 1843 t ORIGIN 1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc 61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt 121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta 181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg actatttacg 241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga 301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt 361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg 421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc 481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg 541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat 601 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac 661 gactcactat agggagaccc aagcttacca ccatggacac taaggatgac gatgacaaag 721 cggccatcac aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat ataaatatca 781 atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa acacaacata 841 tccagtcact atggcggccg cattaggcac cccaggcttt acactttatg cttccggctc 901 gtataatgtg tggattttga gttaggatcc ggcgagattt tcaggagcta aggaagctaa 961 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga 1021 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga 1081 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat 1141 tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg 1201 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga 1261 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata 1321 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga 1381 gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt 1441 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg 1501 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtctgtg atggcttcca 1561 tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta 1621 aacgcgtgga tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt 1681 ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtgtgct 1741 atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat 1801 atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc 1861 gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg 1921 aaatgaacgg ctcttttgct gacgagaaca gggactggtg aaatgcagtt taaggtttac 1981 acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac 2041 acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc 2101 tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc 2161 gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc 2221 gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc 2281 cttatacaca gccagtctgc aggtcgacca tagtgactgg atatgttgtg ttttacagta 2341 ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta 2401 cgtttctcgt tcagctttct tgtacaaagt ggtgatcgaa cagaagctga tatcggaaga 2461 ggacctgaac tagtaactcg agcatgcatc tagagggccc tattctatag tgtcacctaa 2521 atgctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt 2581 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat 2641 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg 2701 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg 2761 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg 2821 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta 2881 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt 2941 tcgccggctt tccccgtcaa gctctaaatc ggggcatccc tttagggttc cgatttagtg 3001 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat 3061 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac 3121 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag 3181 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 3241 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc 3301 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac 3361 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 3421 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta 3481 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc 3541 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg 3601 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca 3661 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt 3721 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg 3781 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga 3841 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca 3901 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct 3961 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac 4021 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc 4081 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt 4141 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt 4201 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg 4261 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat 4321 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc 4381 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc 4441 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc 4501 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt 4561 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 4621 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 4681 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg 4741 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa 4801 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct 4861 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 4921 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 4981 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 5041 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 5101 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 5161 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 5221 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 5281 tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc 5341 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 5401 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 5461 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 5521 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 5581 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 5641 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 5701 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 5761 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 5821 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 5881 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 5941 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 6001 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 6061 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 6121 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 6181 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 6241 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 6301 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 6361 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 6421 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 6481 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 6541 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 6601 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 6661 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 6721 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 6781 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 6841 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg 6901 atcccctatg gtcgactctc agtacaatct gctctgatgc cgcatagtta agccagtatc 6961 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa 7021 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc 7081 tgcttcg //