LOCUS LT727164 7034 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian vector pdcDNA-FLAG, complete sequence. ACCESSION LT727164 VERSION LT727164.1 KEYWORDS . SOURCE Mammalian vector pdcDNA-FLAG ORGANISM Mammalian vector pdcDNA-FLAG other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 7034) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..7034 /organism="Mammalian vector pdcDNA-FLAG" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 4704." /db_xref="taxon:1945170" regulatory 27..610 /note="hCMV-IE promoter and enhancer" /regulatory_class="promoter" regulatory 655..671 /note="T7 promoter" /regulatory_class="promoter" misc_feature 696..719 /note="FLAG epitope" regulatory complement(729..853) /note="attR1" /regulatory_class="attenuator" regulatory 861..913 /note="lacUV5 promoter" /regulatory_class="promoter" CDS 962..1621 /transl_table=11 /note="chloramphenicol resistance" /protein_id="SJL87633.1" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A" CDS 1963..2268 /transl_table=11 /note="ccdB" /protein_id="SJL87634.1" /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" regulatory 2309..2433 /note="attR2" /regulatory_class="attenuator" regulatory complement(2453..2469) /note="SP6 promoter" /regulatory_class="promoter" regulatory 2495..2719 /note="BGH polyA" /regulatory_class="terminator" rep_origin 2765..3193 /note="f1 ori" regulatory 3198..3407 /note="SV40 early promoter" /regulatory_class="promoter" rep_origin 3364..3439 /note="SV40 ori" CDS 3531..4325 /transl_table=11 /note="Tn5 neomycin resistance" /protein_id="SJL87635.1" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" regulatory 4499..4573 /note="SV40 polyA early" /regulatory_class="terminator" regulatory complement(4499..4573) /note="SV40 polyA late" /regulatory_class="terminator" regulatory complement(4712..4795) /note="lac promoter" /regulatory_class="promoter" rep_origin complement(4887..5289) /note="pMB1 ori" CDS complement(5830..6690) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL87636.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" BASE COUNT 1710 a 1732 c 1758 g 1834 t ORIGIN 1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc 61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt 121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta 181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg actatttacg 241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga 301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt 361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg 421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc 481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg 541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat 601 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac 661 gactcactat agggagaccc aagcttacca ccatggacac taaggatgac gatgacaaag 721 cggccatcac aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat ataaatatca 781 atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa acacaacata 841 tccagtcact atggcggccg cattaggcac cccaggcttt acactttatg cttccggctc 901 gtataatgtg tggattttga gttaggatcc ggcgagattt tcaggagcta aggaagctaa 961 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga 1021 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga 1081 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat 1141 tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg 1201 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga 1261 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata 1321 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga 1381 gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt 1441 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg 1501 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtctgtg atggcttcca 1561 tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta 1621 aacgcgtgga tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt 1681 ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtgtgct 1741 atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat 1801 atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc 1861 gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg 1921 aaatgaacgg ctcttttgct gacgagaaca gggactggtg aaatgcagtt taaggtttac 1981 acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac 2041 acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc 2101 tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc 2161 gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc 2221 gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc 2281 cttatacaca gccagtctgc aggtcgacca tagtgactgg atatgttgtg ttttacagta 2341 ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta 2401 cgtttctcgt tcagctttct tgtacaaagt ggtgatctag agggccctat tctatagtgt 2461 cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct 2521 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt 2581 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg 2641 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg 2701 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat 2761 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg 2821 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc 2881 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg gcatcccttt agggttccga 2941 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt 3001 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat 3061 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat 3121 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa 3181 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct 3241 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc 3301 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg 3361 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc 3421 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt 3481 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac 3541 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact 3601 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc 3661 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg 3721 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg 3781 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt 3841 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc 3901 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag 3961 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg 4021 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc 4081 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt 4141 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg 4201 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt 4261 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct 4321 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg 4381 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga 4441 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa 4501 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 4561 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 4621 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt 4681 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 4741 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 4801 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc 4861 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 4921 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 4981 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 5041 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 5101 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 5161 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 5221 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag 5281 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 5341 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 5401 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 5461 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 5521 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 5581 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 5641 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 5701 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 5761 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 5821 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 5881 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 5941 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 6001 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 6061 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 6121 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 6181 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 6241 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 6301 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 6361 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 6421 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 6481 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 6541 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 6601 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 6661 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 6721 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 6781 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga 6841 tctcccgatc ccctatggtc gactctcagt acaatctgct ctgatgccgc atagttaagc 6901 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa 6961 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt 7021 tttgcgctgc ttcg //