LOCUS LT727162 4569 bp DNA circular SYN 06-FEB-2017
DEFINITION Reporter vector p110huIL6P-luc+, complete sequence.
ACCESSION LT727162
VERSION LT727162.1
KEYWORDS .
SOURCE Reporter vector p110huIL6P-luc+
ORGANISM Reporter vector p110huIL6P-luc+
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4569)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..4569
/organism="Reporter vector p110huIL6P-luc+"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4693."
/db_xref="taxon:1945185"
CDS 54..1706
/transl_table=11
/note="LUCm"
/protein_id="SJL87629.1"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
regulatory 1794..1890
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory complement(1794..1890)
/note="SV40 polyA early"
/regulatory_class="terminator"
rep_origin complement(2103..2505)
/note="pMB1 ori"
CDS complement(3046..3906)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87630.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory complement(4253..4349)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 4253..4349
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory 4448..4558
/note="hIL6 promoter fragment"
/regulatory_class="promoter"
BASE COUNT 1226 a 1066 c 1064 g 1213 t
ORIGIN
1 gagatctgcg atctaagtaa gcttggcatt ccggtactgt tggtaaagcc accatggaag
61 acgccaaaaa cataaagaaa ggcccggcgc cattctatcc gctggaagat ggaaccgctg
121 gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca attgctttta
181 cagatgcaca tatcgaggtg gacatcactt acgctgagta cttcgaaatg tccgttcggt
241 tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc gtatgcagtg
301 aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga gttgcagttg
361 cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgggc atttcgcagc
421 ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg caaaaaaagc
481 tcccaatcat ccaaaaaatt attatcatgg attctaaaac ggattaccag ggatttcagt
541 cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac gattttgtgc
601 cagagtcctt cgatagggac aagacaattg cactgatcat gaactcctct ggatctactg
661 gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg cgtgagattc tcgcatgcca
721 gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt gttgttccat
781 tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga tttcgagtcg
841 tcttaatgta tagatttgaa gaagagctgt ttctgaggag ccttcaggat tacaagattc
901 aaagtgcgct gctggtgcca accctattct ccttcttcgc caaaagcact ctgattgaca
961 aatacgattt atctaattta cacgaaattg cttctggtgg cgctcccctc tctaaggaag
1021 tcggggaagc ggttgccaag aggttccatc tgccaggtat caggcaagga tatgggctca
1081 ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg ggcgcggtcg
1141 gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg aaaacgctgg
1201 gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat gattatgtcc ggttatgtaa
1261 acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat tctggagaca
1321 tagcttactg ggacgaagac gaacacttct tcatcgttga ccgcctgaag tctctgatta
1381 agtacaaagg ctatcaggtg gctcccgctg aattggaatc catcttgctc caacacccca
1441 acatcttcga cgcaggtgtc gcaggtcttc ccgacgatga cgccggtgaa cttcccgccg
1501 ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg gattacgtcg
1561 ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg gacgaagtac
1621 cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc ataaaggcca
1681 agaagggcgg aaagatcgcc gtgtaattct agagtcgggg cggccggccg cttcgagcag
1741 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
1801 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
1861 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
1921 aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgtcga
1981 ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact
2041 atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca
2101 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
2161 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
2221 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
2281 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
2341 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
2401 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
2461 gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
2521 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
2581 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
2641 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
2701 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
2761 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
2821 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
2881 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
2941 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
3001 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
3061 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
3121 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
3181 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
3241 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
3301 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
3361 tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
3421 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
3481 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
3541 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
3601 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
3661 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
3721 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
3781 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
3841 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
3901 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
3961 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
4021 ccgaaaagtg ccacctgaac ttgtttattg cagcttataa tggttacaaa taaagcaata
4081 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
4141 aactcatcaa tgtatcttat catgtctgga tcataatcag ccataccaca tttgtagagg
4201 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
4261 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
4321 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
4381 tcatcaatgt atcttatcat gtctggatct cgaagcttgc atgcctgcag gtcgactcta
4441 gaggatcgcc ccaccctcac cctccaacaa agatttatca aatgtgggat tttcccatga
4501 gtctcaatat tagagtctca acccccaata aatataggac tggagatgtc tgaggctcat
4561 tctgccctc
//