LOCUS LT727161 4919 bp DNA circular SYN 06-FEB-2017
DEFINITION Reporter vector p50huIL6P-luc+, complete sequence.
ACCESSION LT727161
VERSION LT727161.1
KEYWORDS .
SOURCE Reporter vector p50huIL6P-luc+
ORGANISM Reporter vector p50huIL6P-luc+
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4919)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..4919
/organism="Reporter vector p50huIL6P-luc+"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4692."
/db_xref="taxon:1945197"
regulatory 88..137
/note="hIL6 promoter fragment"
/regulatory_class="promoter"
CDS 185..1837
/transl_table=11
/note="LUCm"
/protein_id="SJL87627.1"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
regulatory 1925..2021
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory complement(1925..2021)
/note="SV40 polyA early"
/regulatory_class="terminator"
rep_origin complement(2238..2640)
/note="pMB1 ori"
CDS complement(3181..4041)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87628.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
rep_origin 4173..4628
/note="f1 ori"
regulatory complement(4759..4912)
/note="synthetic polyA"
/regulatory_class="terminator"
BASE COUNT 1277 a 1172 c 1183 g 1287 t
ORIGIN
1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctaa gtaagcttgc
61 atgcctgcag gtcgactcta gaggatcatt agagtctcaa cccccaataa atataggact
121 ggagatgtct gaggctcatt ctgccctcga agcttggcat tccggtactg ttggtaaagc
181 caccatggaa gacgccaaaa acataaagaa aggcccggcg ccattctatc cgctggaaga
241 tggaaccgct ggagagcaac tgcataaggc tatgaagaga tacgccctgg ttcctggaac
301 aattgctttt acagatgcac atatcgaggt ggacatcact tacgctgagt acttcgaaat
361 gtccgttcgg ttggcagaag ctatgaaacg atatgggctg aatacaaatc acagaatcgt
421 cgtatgcagt gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg
481 agttgcagtt gcgcccgcga acgacattta taatgaacgt gaattgctca acagtatggg
541 catttcgcag cctaccgtgg tgttcgtttc caaaaagggg ttgcaaaaaa ttttgaacgt
601 gcaaaaaaag ctcccaatca tccaaaaaat tattatcatg gattctaaaa cggattacca
661 gggatttcag tcgatgtaca cgttcgtcac atctcatcta cctcccggtt ttaatgaata
721 cgattttgtg ccagagtcct tcgataggga caagacaatt gcactgatca tgaactcctc
781 tggatctact ggtctgccta aaggtgtcgc tctgcctcat agaactgcct gcgtgagatt
841 ctcgcatgcc agagatccta tttttggcaa tcaaatcatt ccggatactg cgattttaag
901 tgttgttcca ttccatcacg gttttggaat gtttactaca ctcggatatt tgatatgtgg
961 atttcgagtc gtcttaatgt atagatttga agaagagctg tttctgagga gccttcagga
1021 ttacaagatt caaagtgcgc tgctggtgcc aaccctattc tccttcttcg ccaaaagcac
1081 tctgattgac aaatacgatt tatctaattt acacgaaatt gcttctggtg gcgctcccct
1141 ctctaaggaa gtcggggaag cggttgccaa gaggttccat ctgccaggta tcaggcaagg
1201 atatgggctc actgagacta catcagctat tctgattaca cccgaggggg atgataaacc
1261 gggcgcggtc ggtaaagttg ttccattttt tgaagcgaag gttgtggatc tggataccgg
1321 gaaaacgctg ggcgttaatc aaagaggcga actgtgtgtg agaggtccta tgattatgtc
1381 cggttatgta aacaatccgg aagcgaccaa cgccttgatt gacaaggatg gatggctaca
1441 ttctggagac atagcttact gggacgaaga cgaacacttc ttcatcgttg accgcctgaa
1501 gtctctgatt aagtacaaag gctatcaggt ggctcccgct gaattggaat ccatcttgct
1561 ccaacacccc aacatcttcg acgcaggtgt cgcaggtctt cccgacgatg acgccggtga
1621 acttcccgcc gccgttgttg ttttggagca cggaaagacg atgacggaaa aagagatcgt
1681 ggattacgtc gccagtcaag taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt
1741 ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca gagagatcct
1801 cataaaggcc aagaagggcg gaaagatcgc cgtgtaattc tagagtcggg gcggccggcc
1861 gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
1921 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
1981 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
2041 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta aaatcgataa
2101 ggatccgtcg atcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg
2161 gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta
2221 ggacaggtgc cggcagcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
2281 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
2341 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
2401 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
2461 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
2521 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
2581 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
2641 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
2701 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
2761 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
2821 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
2881 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
2941 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
3001 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
3061 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
3121 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
3181 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
3241 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
3301 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
3361 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
3421 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
3481 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
3541 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
3601 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
3661 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
3721 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
3781 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
3841 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
3901 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
3961 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
4021 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
4081 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
4141 tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc
4201 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
4261 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
4321 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
4381 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
4441 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
4501 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
4561 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
4621 tacaatttcc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
4681 ctcttcgcta ttacgccagc ccaagctacc atgataagta agtaatatta aggtacggga
4741 ggtacttgga gcggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt
4801 ttgtgtgaat cgatagtact aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa
4861 ctagcaaaat aggctgtccc cagtgcaagt gcaggtgcca gaacatttct ctatcgata
//