LOCUS LT727159 5855 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian expression vector pCAGGS-EYopT-C139S, complete sequence. ACCESSION LT727159 VERSION LT727159.1 KEYWORDS . SOURCE Mammalian expression vector pCAGGS-EYopT-C139S ORGANISM Mammalian expression vector pCAGGS-EYopT-C139S other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 5855) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..5855 /organism="Mammalian expression vector pCAGGS-EYopT-C139S" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 4667." /db_xref="taxon:1944824" misc_feature 16..54 /note="E-tag" CDS <64..1029 /codon_start=1 /transl_table=11 /note="YopT-C139S" /protein_id="SJL87623.1" /translation="DSIHGHYHIQLSNYSAGENLQSATLTEGVIGAHRVKVETALSHS NRQKKLSATIKHNQSSRSMLDRKLTSDGKANQRSSFTFSMIMYRMIHFVLSTRVPAVR ESVANYGGNINFKFAQTKGAFLHQIIKHSDTASGVSEALCAHWIWSHAQGQSLFDQLY VGGRKGKFQIDTLYSIKQLQIDGCKADVDQDEVTLDWFKKKGISERMIERHCLLRPVD VTGTTESEGPDQLLNAILDTHGIGYGYKKIYLSGQMSAHAIAAYVNEKSGVTFFDPNF GEFHFSDKEKFRKWFTNSFWENSMYHYPLGVGQRFSVLTFDSKEV" regulatory 1145..1236 /note="beta-globin polyA" /regulatory_class="terminator" misc_feature 1237..1593 /note="3' FR of beta-globin" regulatory complement(1671..1753) /note="lac promoter" /regulatory_class="promoter" rep_origin 1895..1970 /note="SV40 ori" regulatory 2001..2075 /note="SV40 polyA early" /regulatory_class="terminator" regulatory complement(2001..2075) /note="SV40 polyA late" /regulatory_class="terminator" rep_origin complement(2190..2591) /note="pMB1 ori" CDS complement(3132..3992) /transl_table=11 /note="ampicillin" /protein_id="SJL87624.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" regulatory 4128..4505 /note="hCMV-IE enhancer" /regulatory_class="promoter" regulatory 4511..5852 /note="AG promoter" /regulatory_class="promoter" misc_feature 4880..5804 /note="intron AG promoter" BASE COUNT 1310 a 1500 c 1586 g 1459 t ORIGIN 1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 61 gctgacagta ttcacggaca ctaccatatc caactatcga attattctgc cggtgaaaac 121 cttcaatcag ctacactcac cgaaggggtg attggcgcac accgagtgaa agtggaaaca 181 gcactgtcac actcaaaccg gcagaaaaag ttatcagcta ccataaaaca taaccagtca 241 agccgttcta tgctggatag aaagttgacc agcgacggca aagctaacca acgaagcagc 301 tttaccttca gtatgattat gtatcgcatg atacattttg tactcagtac tcgtgtgccc 361 gcggtgagag agtctgttgc aaattacgga ggtaacatca atttcaagtt tgctcagacc 421 aaaggggctt ttcttcatca aataataaaa cattcagaca ccgctagcgg tgtcagtgag 481 gctttatgtg cacattggat ttggagtcat gcacagggcc aaagcttatt tgaccagctc 541 tatgttggcg ggcgtaaagg gaaattccag atcgatacac tttactcaat taaacagttg 601 caaatagatg gttgtaaagc agacgttgat caagatgagg taacactaga ttggttcaag 661 aaaaagggca tatcagaacg tatgattgaa cggcattgct tactgcgtcc agttgatgtt 721 actggtacga cggaatcaga agggccggat caattattaa acgctatcct tgatactcat 781 gggataggtt acggttataa aaaaatatat ctctcaggcc aaatgtcagc ccacgccata 841 gcggcgtatg tcaacgaaaa gagtggtgtt actttcttcg atcccaattt cggtgaattc 901 cacttttctg ataaggaaaa gttccgcaaa tggtttacta actcattctg ggaaaattct 961 atgtatcatt atcctctggg ggtggggcag cgttttagcg tgttaacatt tgactccaag 1021 gaggtttgac ccgggacatt aaacgcgtgg gtaccggatc cggcaattca ctcctcaggt 1081 gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca 1141 ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct 1201 gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg 1261 tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt 1321 ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata 1381 aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc 1441 cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc 1501 cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc 1561 agtcatagct gtccctcttc tcttatggag atccctcgac ctgcagccca agcttggcgt 1621 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 1681 tacgagccgg aagcataaag tgtaaagcct gggtgcctaa tgagtgagct aactcacatt 1741 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agcggatccg 1801 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact 1861 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 1921 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 1981 ctaggctttt gcaaaaagct aacttgttta ttgcagctta taatggttac aaataaagca 2041 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 2101 ccaaactcat caatgtatct tatcatgtct ggatccgctg cattaatgaa tcggccaacg 2161 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 2221 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 2281 atccacagaa tcagggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 2341 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 2401 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 2461 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 2521 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 2581 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 2641 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 2701 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 2761 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta 2821 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 2881 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 2941 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 3001 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 3061 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 3121 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 3181 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 3241 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 3301 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 3361 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 3421 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 3481 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 3541 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 3601 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 3661 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 3721 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 3781 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 3841 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 3901 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 3961 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 4021 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 4081 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgggtcgac attgattatt 4141 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt 4201 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc 4261 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg 4321 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat 4381 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca 4441 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat 4501 taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc 4561 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg 4621 gggggggggg ccccccccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg 4681 gaaaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag 4741 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgttgcgcg 4801 ctgccttccc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact 4861 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta 4921 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct 4981 ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt 5041 ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc 5101 ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg 5161 gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg 5221 agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt 5281 tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc 5341 cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc 5401 cggggagggc tcgggggaag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg 5461 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt 5521 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc 5581 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt 5641 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct 5701 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta 5761 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc 5821 tggttattgt gctgtctcat cattttggca aagaa //