LOCUS LT727156 5546 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-EYopE, complete sequence.
ACCESSION LT727156
VERSION LT727156.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-EYopE
ORGANISM Mammalian expression vector pCAGGS-EYopE
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5546)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5546
/organism="Mammalian expression vector pCAGGS-EYopE"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4664."
/db_xref="taxon:1944820"
misc_feature 16..54
/note="E-tag"
CDS <64..720
/codon_start=1
/transl_table=11
/note="YopE"
/protein_id="SJL87617.1"
/translation="KISSFISTSLPLPASVSGSSSVGEMSGRSVSQQKSDQYANNLAG
RTESPQGSSLASRIIERLSSMAHSVIGFIQRMFSEGSHKPVVTPALTPAQMPSPTSFS
DSIKQLAAETLPKYMQQLSSLDAETLQKNHDQFATGSGPLRGSITQCQGLMQFCGGEL
QAEASAILNTPVCGIPFSQWGTVGGAASAYVASGVDLTQAANEIKGLGQQMQQLLSLM
"
regulatory 836..927
/note="beta-globin polyA"
/regulatory_class="terminator"
misc_feature 928..1284
/note="3' FR of beta-globin"
regulatory complement(1362..1444)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1586..1661
/note="SV40 ori"
regulatory 1692..1766
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(1692..1766)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(1881..2282)
/note="pMB1 ori"
CDS complement(2823..3683)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87618.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3819..4196
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 4202..5543
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4571..5495
/note="intron AG promoter"
BASE COUNT 1184 a 1464 c 1544 g 1354 t
ORIGIN
1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
61 gctaaaatat catcatttat ttctacatca ctgcccctgc cggcatcagt gtcaggatct
121 agcagcgtag gagaaatgtc tgggcgctca gtctcacagc aaaaaagtga tcaatatgca
181 aacaatctgg ccgggcgcac tgaaagccct cagggttcca gcttagccag ccgtatcatt
241 gagaggttat catcaatggc ccactctgtg attggattta tccaacgcat gttctcggag
301 gggagccata aaccggtggt gacaccagca ctcacgcctg cacaaatgcc aagccctacg
361 tctttcagtg atagtatcaa gcaacttgct gctgagacgc tgccaaaata catgcagcag
421 ttgagtagct tggatgcaga gacgctgcag aaaaatcatg accagttcgc cacgggcagc
481 ggccctcttc gtggcagtat cactcaatgc caagggctga tgcagttttg tggtggggaa
541 ttgcaagctg aggccagtgc cattttaaac acgcctgttt gtggtattcc cttctcgcag
601 tggggaactg ttggtggggc ggccagcgcg tacgtcgcca gtggcgttga tctaacgcag
661 gcagcaaatg agatcaaagg gctggggcaa cagatgcagc aattactgtc attgatgtga
721 cccgggacat taaacgcgtg ggtaccggat ccggcaattc actcctcagg tgcaggctgc
781 ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct
841 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg
901 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact
961 cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag
1021 tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca
1081 tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg
1141 aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt
1201 ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc
1261 tgtccctctt ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt
1321 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
1381 gaagcataaa gtgtaaagcc tgggtgccta atgagtgagc taactcacat taattgcgtt
1441 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat
1501 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
1561 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
1621 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
1681 tgcaaaaagc taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
1741 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
1801 tcaatgtatc ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag
1861 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
1921 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
1981 atcagggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
2041 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
2101 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
2161 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
2221 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
2281 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
2341 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
2401 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
2461 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
2521 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
2581 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
2641 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
2701 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
2761 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
2821 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
2881 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
2941 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
3001 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
3061 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
3121 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
3181 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
3241 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
3301 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
3361 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
3421 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
3481 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
3541 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
3601 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
3661 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
3721 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
3781 gttccgcgca catttccccg aaaagtgcca cctgggtcga cattgattat tgactagtta
3841 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
3901 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
3961 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
4021 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
4081 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
4141 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
4201 cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat
4261 tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggg
4321 gcccccccca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggaaaggtgc
4381 ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg
4441 gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgttgcgc gctgccttcc
4501 ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt
4561 actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt
4621 ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg
4681 ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc
4741 cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg
4801 tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
4861 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg
4921 tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca
4981 cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg
5041 cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg
5101 ctcgggggaa ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc
5161 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa
5221 tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga
5281 agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc
5341 gccgtcccct tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg
5401 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg
5461 ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg
5521 tgctgtctca tcattttggc aaagaa
//