LOCUS LT727154 5462 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian expression vector pCAGGS-EhCdc42-DP, complete sequence. ACCESSION LT727154 VERSION LT727154.1 KEYWORDS . SOURCE Mammalian expression vector pCAGGS-EhCdc42-DP ORGANISM Mammalian expression vector pCAGGS-EhCdc42-DP other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 5462) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..5462 /organism="Mammalian expression vector pCAGGS-EhCdc42-DP" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 4662." /db_xref="taxon:1944776" misc_feature 16..54 /note="E-tag" CDS <64..636 /codon_start=1 /transl_table=11 /note="hCdc42-DP" /protein_id="SJL87613.1" /translation="QTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVM IGGEPYTLGLFDTAGLEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITH HCPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALT QKGLKNVFDEAILAALEPPEPKKSRRCVVL" regulatory 752..843 /note="beta-globin polyA" /regulatory_class="terminator" misc_feature 844..1200 /note="3' FR of beta-globin" regulatory complement(1278..1360) /note="lac promoter" /regulatory_class="promoter" rep_origin 1502..1577 /note="SV40 ori" regulatory complement(1608..1682) /note="SV40 polyA late" /regulatory_class="terminator" regulatory 1608..1682 /note="SV40 polyA early" /regulatory_class="terminator" rep_origin complement(1797..2198) /note="pMB1 ori" CDS complement(2739..3599) /transl_table=11 /note="ampicillin" /protein_id="SJL87614.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" regulatory 3735..4112 /note="hCMV-IE enhancer" /regulatory_class="promoter" regulatory 4118..5459 /note="AG promoter" /regulatory_class="promoter" misc_feature 4487..5411 /note="intron" BASE COUNT 1180 a 1423 c 1499 g 1360 t ORIGIN 1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 61 gctcagacaa ttaagtgtgt tgttgtgggc gatggtgctg ttggtaaaac atgtctcctg 121 atatcctaca caacaaacaa atttccatcg gaatatgtac cgactgtttt tgacaactat 181 gcagtcacag ttatgattgg tggagaacca tatactcttg gactttttga tactgcaggg 241 ctcgaggatt atgacagatt acgaccgctg agttatccac aaacagatgt atttctagtc 301 tgtttttcag tggtctctcc atcttcattt gaaaacgtga aagaaaagtg ggtgcctgag 361 ataactcacc actgtccaaa gactcctttc ttgcttgttg ggactcaaat tgatctcaga 421 gatgacccct ctactattga gaaacttgcc aagaacaaac agaagcctat cactccagag 481 actgctgaaa agctggcccg tgacctgaag gctgtcaagt atgtggagtg ttctgcactt 541 acacagaaag gcctaaagaa tgtatttgac gaagcaatat tggctgccct ggagcctcca 601 gaaccgaaga agagccgcag gtgtgtggtg ctatgacccg ggacattaaa cgcgtgggta 661 ccggatccgg caattcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt 721 ggccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca aaaattatgg 781 ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat 841 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa 901 tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct 961 ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc 1021 tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg 1081 ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag 1141 atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc 1201 cctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 1261 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 1321 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 1381 gggaaacctg tcgtgccagc ggatccgcat ctcaattagt cagcaaccat agtcccgccc 1441 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc 1501 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag 1561 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctaac ttgtttattg 1621 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 1681 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga 1741 tccgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 1801 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 1861 agctcactca aaggcggtaa tacggttatc cacagaatca gggataacgc aggaaagaac 1921 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 1981 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 2041 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 2101 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 2161 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 2221 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 2281 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 2341 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 2401 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc 2461 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 2521 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 2581 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 2641 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 2701 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 2761 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 2821 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 2881 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 2941 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 3001 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 3061 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 3121 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 3181 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 3241 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 3301 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 3361 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 3421 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 3481 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 3541 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 3601 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 3661 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 3721 gtgccacctg ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc 3781 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc 3841 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt 3901 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca 3961 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg 4021 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca 4081 gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt 4141 cactctcccc atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt 4201 attttgtgca gcgatggggg cggggggggg gggggggccc cccccaggcg gggcggggcg 4261 gggcgagggg cggggcgggg cgaggcggaa aggtgcggcg gcagccaatc agagcggcgc 4321 gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag 4381 cgcgcggcgg gcgggagtcg ttgcgcgctg ccttcccccc gtgccccgct ccgccgccgc 4441 ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga 4501 cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct 4561 gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc 4621 ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg 4681 cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg 4741 gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg 4801 cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca 4861 accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct 4921 ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt 4981 gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaagggg cgcggcggcc 5041 cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat 5101 cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga 5161 ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg 5221 aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc 5281 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg 5341 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct 5401 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag 5461 aa //