LOCUS LT727153 5462 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-EhCdc42-DN, complete sequence.
ACCESSION LT727153
VERSION LT727153.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-EhCdc42-DN
ORGANISM Mammalian expression vector pCAGGS-EhCdc42-DN
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5462)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5462
/organism="Mammalian expression vector pCAGGS-EhCdc42-DN"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4661."
/db_xref="taxon:1944775"
misc_feature 16..54
/note="E-tag"
CDS <64..636
/codon_start=1
/transl_table=11
/note="human CDC42-DN"
/protein_id="SJL87611.1"
/translation="QTIKCVVVGDGAVGKNCLLISYTTNKFPSEYVPTVFDNYAVTVM
IGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITH
HCPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALT
QKGLKNVFDEAILAALEPPEPKKSRRCVVL"
regulatory 752..843
/note="3' UTR of rabbit beta-globin, incl. polyA"
/regulatory_class="terminator"
misc_feature 844..1200
/note="3' flanking region of rabbit beta-globin"
regulatory complement(1278..1360)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1502..1577
/note="SV40 ori"
regulatory complement(1608..1682)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 1608..1682
/note="SV40 polyA early"
/regulatory_class="terminator"
rep_origin complement(1797..2198)
/note="pMB1 ori"
CDS complement(2739..3599)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87612.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3735..4112
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 4118..5459
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4487..5411
/note="intron"
BASE COUNT 1182 a 1422 c 1499 g 1359 t
ORIGIN
1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
61 gctcagacaa ttaagtgtgt tgttgtgggc gatggtgctg ttggtaaaaa ctgtctcctg
121 atatcctaca caacaaacaa atttccatcg gaatatgtac cgactgtttt tgacaactat
181 gcagtcacag ttatgattgg tggagaacca tatactcttg gactttttga tactgcaggg
241 caagaggatt atgacagatt acgaccgctg agttatccac aaacagatgt atttctagtc
301 tgtttttcag tggtctctcc atcttcattt gaaaacgtga aagaaaagtg ggtgcctgag
361 ataactcacc actgtccaaa gactcctttc ttgcttgttg ggactcaaat tgatctcaga
421 gatgacccct ctactattga gaaacttgcc aagaacaaac agaagcctat cactccagag
481 actgctgaaa agctggcccg tgacctgaag gctgtcaagt atgtggagtg ttctgcactt
541 acacagaaag gcctaaagaa tgtatttgac gaagcaatat tggctgccct ggagcctcca
601 gaaccgaaga agagccgcag gtgtgtggtg ctatgacccg ggacattaaa cgcgtgggta
661 ccggatccgg caattcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt
721 ggccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca aaaattatgg
781 ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat
841 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa
901 tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct
961 ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc
1021 tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg
1081 ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag
1141 atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc
1201 cctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
1261 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
1321 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
1381 gggaaacctg tcgtgccagc ggatccgcat ctcaattagt cagcaaccat agtcccgccc
1441 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
1501 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
1561 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctaac ttgtttattg
1621 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
1681 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
1741 tccgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
1801 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
1861 agctcactca aaggcggtaa tacggttatc cacagaatca gggataacgc aggaaagaac
1921 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
1981 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
2041 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
2101 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
2161 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
2221 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
2281 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
2341 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
2401 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
2461 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
2521 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
2581 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
2641 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
2701 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
2761 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
2821 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
2881 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
2941 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
3001 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
3061 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
3121 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
3181 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
3241 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
3301 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
3361 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
3421 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
3481 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
3541 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
3601 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
3661 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
3721 gtgccacctg ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
3781 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
3841 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
3901 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
3961 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
4021 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
4081 gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt
4141 cactctcccc atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt
4201 attttgtgca gcgatggggg cggggggggg gggggggccc cccccaggcg gggcggggcg
4261 gggcgagggg cggggcgggg cgaggcggaa aggtgcggcg gcagccaatc agagcggcgc
4321 gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag
4381 cgcgcggcgg gcgggagtcg ttgcgcgctg ccttcccccc gtgccccgct ccgccgccgc
4441 ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga
4501 cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct
4561 gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc
4621 ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg
4681 cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg
4741 gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg
4801 cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca
4861 accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct
4921 ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt
4981 gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaagggg cgcggcggcc
5041 cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat
5101 cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga
5161 ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg
5221 aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc
5281 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
5341 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
5401 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
5461 aa
//