LOCUS LT727149 5468 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian expression vector pCAGGS-EhRhoA-DN, complete sequence. ACCESSION LT727149 VERSION LT727149.1 KEYWORDS . SOURCE Mammalian expression vector pCAGGS-EhRhoA-DN ORGANISM Mammalian expression vector pCAGGS-EhRhoA-DN other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 5468) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..5468 /organism="Mammalian expression vector pCAGGS-EhRhoA-DN" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 4657." /db_xref="taxon:1944782" misc_feature 16..54 /note="E-tag" CDS <64..642 /codon_start=1 /transl_table=11 /note="hRhoA-DN" /protein_id="SJL87603.1" /translation="AAIRKKLVIVGDGACGKNCLLIVFSKDQFPEVYVPTVFENYVAD IEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEV KHFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSA KTKDGVREVFEMATRAALQARRGKKKSGCLVL" regulatory 758..849 /note="beta-globin polyA" /regulatory_class="terminator" misc_feature 850..1206 /note="3' FR of beta-globin" regulatory complement(1284..1366) /note="lac promoter" /regulatory_class="promoter" rep_origin 1508..1583 /note="SV40 ori" regulatory complement(1614..1688) /note="SV40 polyA late" /regulatory_class="terminator" regulatory 1614..1688 /note="SV40 polyA early" /regulatory_class="terminator" rep_origin complement(1803..2204) /note="pMB1 ori" CDS complement(2745..3605) /transl_table=11 /note="ampicillin" /protein_id="SJL87604.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" regulatory 3741..4118 /note="hCMV-IE enhancer" /regulatory_class="promoter" regulatory 4124..5465 /note="AG promoter" /regulatory_class="promoter" misc_feature 4493..5417 /note="intron" BASE COUNT 1178 a 1415 c 1539 g 1336 t ORIGIN 1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 61 gctgctgcca tccggaagaa actggtgatt gttggtgatg gagcctgtgg aaagaactgc 121 ttgctcatag tcttcagcaa ggaccagttc ccagaggtgt atgtgcccac agtgtttgag 181 aactatgtgg cagatatcga ggtggatgga aagcaggtag agttggcttt gtgggacaca 241 gctgggcagg aagattatga tcgcctgagg cccctctcct acccagatac cgatgttata 301 ctgatgtgtt tttccatcga cagccctgat agtttagaaa acatcccaga aaagtggacc 361 ccagaagtca agcatttctg tcccaacgtg cccatcatcc tggttgggaa taagaaggat 421 cttcggaatg atgagcacac aaggcgggag ctagccaaga tgaagcagga gccggtgaaa 481 cctgaagaag gcagagatat ggcaaacagg attggcgctt ttgggtacat ggagtgttca 541 gcaaagacca aagatggagt gagagaggtt tttgaaatgg ctacgagagc tgctctgcaa 601 gctagacgtg ggaagaaaaa atctggttgc cttgtcttgt gacccgggac attaaacgcg 661 tgggtaccgg atccggcaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc 721 tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa 781 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat 841 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag 901 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca 961 tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc 1021 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat 1081 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact 1141 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg 1201 gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt 1261 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 1321 cctgggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 1381 ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc 1441 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc 1501 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 1561 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt 1621 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 1681 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 1741 tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 1801 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 1861 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcaggga taacgcagga 1921 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 1981 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 2041 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 2101 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 2161 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 2221 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 2281 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 2341 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 2401 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 2461 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 2521 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 2581 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 2641 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 2701 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 2761 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 2821 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 2881 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 2941 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 3001 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 3061 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 3121 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 3181 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 3241 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 3301 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 3361 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 3421 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 3481 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 3541 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 3601 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 3661 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 3721 cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt aatcaattac 3781 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg 3841 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc 3901 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac 3961 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa 4021 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac 4081 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt 4141 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt 4201 ttaattattt tgtgcagcga tgggggcggg gggggggggg gggccccccc caggcggggc 4261 ggggcggggc gaggggcggg gcggggcgag gcggaaaggt gcggcggcag ccaatcagag 4321 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa 4381 gcgaagcgcg cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc cccgctccgc 4441 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg 4501 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc 4561 ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag 4621 cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct 4681 gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg 4741 cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa 4801 aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg 4861 gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc 4921 ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt 4981 gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aaggggcgcg 5041 gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat 5101 ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat 5161 ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca 5221 ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc 5281 tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg 5341 ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct 5401 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg 5461 gcaaagaa //