LOCUS       LT727149                5468 bp    DNA     circular SYN 06-FEB-2017
DEFINITION  Mammalian expression vector pCAGGS-EhRhoA-DN, complete sequence.
ACCESSION   LT727149
VERSION     LT727149.1
KEYWORDS    .
SOURCE      Mammalian expression vector pCAGGS-EhRhoA-DN
  ORGANISM  Mammalian expression vector pCAGGS-EhRhoA-DN
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 5468)
  AUTHORS   De Schamphelaire W.
  JOURNAL   Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM.
REFERENCE   2
  AUTHORS   De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
            Roggeman Fonseca M., Vanhoucke M., Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished.
FEATURES             Location/Qualifiers
     source          1..5468
                     /organism="Mammalian expression vector pCAGGS-EhRhoA-DN"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent
                     University,Belgium) accession number LMBP 4657."
                     /db_xref="taxon:1944782"
     misc_feature    16..54
                     /note="E-tag"
     CDS             <64..642
                     /codon_start=1
                     /transl_table=11
                     /note="hRhoA-DN"
                     /protein_id="SJL87603.1"
                     /translation="AAIRKKLVIVGDGACGKNCLLIVFSKDQFPEVYVPTVFENYVAD
                     IEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEV
                     KHFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSA
                     KTKDGVREVFEMATRAALQARRGKKKSGCLVL"
     regulatory      758..849
                     /note="beta-globin polyA"
                     /regulatory_class="terminator"
     misc_feature    850..1206
                     /note="3' FR of beta-globin"
     regulatory      complement(1284..1366)
                     /note="lac promoter"
                     /regulatory_class="promoter"
     rep_origin      1508..1583
                     /note="SV40 ori"
     regulatory      complement(1614..1688)
                     /note="SV40 polyA late"
                     /regulatory_class="terminator"
     regulatory      1614..1688
                     /note="SV40 polyA early"
                     /regulatory_class="terminator"
     rep_origin      complement(1803..2204)
                     /note="pMB1 ori"
     CDS             complement(2745..3605)
                     /transl_table=11
                     /note="ampicillin"
                     /protein_id="SJL87604.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     regulatory      3741..4118
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     regulatory      4124..5465
                     /note="AG promoter"
                     /regulatory_class="promoter"
     misc_feature    4493..5417
                     /note="intron"
BASE COUNT         1178 a         1415 c         1539 g         1336 t
ORIGIN      
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
       61 gctgctgcca tccggaagaa actggtgatt gttggtgatg gagcctgtgg aaagaactgc
      121 ttgctcatag tcttcagcaa ggaccagttc ccagaggtgt atgtgcccac agtgtttgag
      181 aactatgtgg cagatatcga ggtggatgga aagcaggtag agttggcttt gtgggacaca
      241 gctgggcagg aagattatga tcgcctgagg cccctctcct acccagatac cgatgttata
      301 ctgatgtgtt tttccatcga cagccctgat agtttagaaa acatcccaga aaagtggacc
      361 ccagaagtca agcatttctg tcccaacgtg cccatcatcc tggttgggaa taagaaggat
      421 cttcggaatg atgagcacac aaggcgggag ctagccaaga tgaagcagga gccggtgaaa
      481 cctgaagaag gcagagatat ggcaaacagg attggcgctt ttgggtacat ggagtgttca
      541 gcaaagacca aagatggagt gagagaggtt tttgaaatgg ctacgagagc tgctctgcaa
      601 gctagacgtg ggaagaaaaa atctggttgc cttgtcttgt gacccgggac attaaacgcg
      661 tgggtaccgg atccggcaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc
      721 tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa
      781 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat
      841 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
      901 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca
      961 tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc
     1021 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat
     1081 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact
     1141 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg
     1201 gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     1261 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     1321 cctgggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     1381 ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc
     1441 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     1501 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     1561 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt
     1621 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     1681 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     1741 tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     1801 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     1861 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcaggga taacgcagga
     1921 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     1981 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     2041 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     2101 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     2161 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     2221 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     2281 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     2341 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     2401 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     2461 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     2521 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     2581 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     2641 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     2701 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     2761 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     2821 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     2881 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     2941 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     3001 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     3061 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     3121 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     3181 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     3241 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     3301 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     3361 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     3421 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     3481 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     3541 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     3601 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     3661 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     3721 cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt aatcaattac
     3781 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     3841 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     3901 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     3961 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     4021 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     4081 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt
     4141 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt
     4201 ttaattattt tgtgcagcga tgggggcggg gggggggggg gggccccccc caggcggggc
     4261 ggggcggggc gaggggcggg gcggggcgag gcggaaaggt gcggcggcag ccaatcagag
     4321 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa
     4381 gcgaagcgcg cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc cccgctccgc
     4441 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg
     4501 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc
     4561 ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag
     4621 cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct
     4681 gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg
     4741 cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa
     4801 aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg
     4861 gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc
     4921 ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt
     4981 gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aaggggcgcg
     5041 gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat
     5101 ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat
     5161 ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca
     5221 ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc
     5281 tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg
     5341 ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct
     5401 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg
     5461 gcaaagaa
//