LOCUS LT727139 6986 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pGal4-p65S276C, complete sequence.
ACCESSION LT727139
VERSION LT727139.1
KEYWORDS .
SOURCE Mammalian expression vector pGal4-p65S276C
ORGANISM Mammalian expression vector pGal4-p65S276C
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6986)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6986
/organism="Mammalian expression vector pGal4-p65S276C"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4644."
/db_xref="taxon:1945040"
misc_feature 1..825
/note="SV40 VP1 with internal deletion"
regulatory 826..900
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory complement(826..900)
/note="SV40 polyA early"
/regulatory_class="terminator"
misc_feature complement(934..1723)
/note="3' UTR of hNF-kappaB p65 subunit"
CDS complement(1724..3379)
/transl_table=11
/note="hNF-kappaB p65 mutant"
/protein_id="SJL87574.1"
/translation="MDELFPLIFPAEPAQASGPYVEIIEQPKQRGMRFRYKCEGRSAG
SIPGERSTDTTKTHPTIKINGYTGPGTVRISLVTKDPPHRPHPHELVGKDCRDGFYEA
ELCPDRCIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFQVPIEEQRGDYDLNAVRLC
FQVTVRDPSGRPLRLPPVLPHPIFDNRAPNTAELKICRVNRNSGSCLGGDEIFLLCDK
VQKEDIEVYFTGPGWEARGSFSQADVHRQVAIVFRTPPYADPSLQAPVRVSMQLRRPC
DRELSEPMEFQYLPDTDDRHRIEEKRKRTYETFKSIMKKSPFSGPTDPRPPPRRIAVP
SRSSASVPKPAPQPYPFTSSLSTINYDEFPTMVFPSGQISQASALAPAPPQVLPQAPA
PAPAPAMVSALAQAPAPVPVLAPGPPQAVAPPAPKPTQAGEGTLSEALLQLQFDDEDL
GALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQ
RPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLSQISS"
misc_feature complement(2552..2560)
/note="S276C"
misc_feature complement(3380..3415)
/note="linker"
CDS complement(<3416..>3853)
/codon_start=1
/transl_table=11
/note="GALbd"
/protein_id="SJL87575.1"
/translation="KLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVN
KDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
regulatory complement(3997..4178)
/note="RSV-LTR"
/regulatory_class="promoter"
regulatory 4575..4856
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 4813..4888
/note="SV40 ori"
rep_origin complement(4975..5377)
/note="pMB1 ori"
CDS complement(5918..6778)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87576.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1705 a 1738 c 1828 g 1715 t
ORIGIN
1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg
61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac
121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg
181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc
241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc
301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa
361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg
421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct
481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca
541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc
601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga
661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg
721 tttatgagga cacagaggag cttcctgggg atcgctcgat cgagcgatcc agacatgata
781 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
841 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
901 aattccgcca cggatcaagc ttgatatcga attccttttt tttttttttt cagcttggca
961 acagctttat tagttcagag tagaaagagc aagagtccaa gtgctttgat tgttcactaa
1021 aaactatgcc tcctgactgg agagctgcca gcctgctctc ccccactctt aacaacttac
1081 cctactatta aggcacttga gaagagggga gagcaaggaa gtccagacca aaccccttct
1141 ggatcctgga gagagccagt gctgttgcac tgtttccttc agccatggtt gagcaaggaa
1201 agagccggca gagacctctg tagggcagga aggcagcccc tcaaacgctg gtgttaggca
1261 cagggacaat gccagtgcca tacaggggct ggtatctggg gcgttatttt gattaagctg
1321 taatgaatcc atgatggaag acacttgata aggctttgtg ggctcaaagg ccttacctcc
1381 agcctgcttc tgtctctagg agagtaccag aagctggagg atggggatgg gggaccccac
1441 agttccctac agagaaggga gctgaccatc aggacagggg aaaagtttga gtttccccag
1501 ctcccccctt tccagagaag ttaatgcttc tgcttaagca cctccaaaaa gagagagaga
1561 tacagatact gacaataaaa gaataaaata tggctccccc ctccaaggaa gacatccaca
1621 aagttggggg cagttggaac acaccccacc agaatccgta agtgcttttg gagggcttca
1681 atcccctgca accagtgctc tggggagggc aggcgtcacc cccttaggag ctgatctgac
1741 tcagcagggc tgagaagtcc atgtccgcaa tggaggagaa gtcttcatct cctgaaagga
1801 ggccattggg gagccccggg gcccccagtg gagcaggagc tgggtcgggg ggcctctggg
1861 cccctgtcac taggcgagtt atagcctcag ggtactccat cagcatgggc tcagttgtgt
1921 ggggggccac aggtatgccc tggttcagca gctgctgaaa ctcggagttg tcgacggatg
1981 ccaggtctgt gaacacagct gggtctgtgc tgttgccaag caaggccccc aggtcttcat
2041 catcaaactg cagctgcagc agggcctctg acagcgttcc ttccccagcc tgggtgggct
2101 tgggggcagg tggggccaca gcctgaggag ggcctggggc taggactggg acaggggctg
2161 gggcctgggc cagagctgat accatggctg gagcaggggc aggggctgga gcctggggca
2221 ggacttgggg aggggccggg gccaaggccg aggcctggct gatctgccca gaaggaaaca
2281 ccatggtggg aaactcatca tagttgatgg tgctcaggga tgacgtaaag ggatagggct
2341 ggggtgctgg cttggggaca gaagctgagc tgcgggaagg cacagcaatg cgtcgaggtg
2401 gaggccgggg gtcggtgggt ccgctgaaag gactcttctt catgatgctc ttgaaggtct
2461 catatgtcct tttacgtttc tcctcaatcc ggtgacgatc gtctgtatct ggcaggtact
2521 ggaattccat gggctcactg agctcccggt cgcaaggcct ccgcagctgc atggagacac
2581 gcacaggagc ctgcaggctg gggtctgcgt agggaggggt ccggaacaca atggccactt
2641 gtcggtgcac atcagcttgc gaaaaggagc ctcgggcctc ccagcctggt cccgtgaaat
2701 acacctcaat gtcctctttc tgcaccttgt cacacagtag gaagatctca tccccaccga
2761 ggcagctgcc agagtttcgg ttcactcggc agatcttgag ctcggcagtg ttgggggcac
2821 gattgtcaaa gatgggatga ggaaggacag gcggcaggcg gaggggcctg cctgatgggt
2881 cccgcactgt cacctggaag cagagccgca cagcattcag gtcgtagtcc ccacgctgct
2941 cttctatagg aacttggaag gggttgttgt tggtctggat gcgctgactg atagcctgct
3001 ccaggtcccg cttcttcaca cactggattc ccaggttctg gaaactgtgg atgcagcggt
3061 ccgggcagag ctcagcctca tagaagccat cccggcagtc ctttcctaca agctcgtggg
3121 ggtgaggccg gtgaggaggg tccttggtga ccagggagat gcgcactgtc cctggtcctg
3181 tgtagccatt gatcttgatg gtggggtggg tcttggtggt atctgtgctc ctctcgcctg
3241 ggatgctgcc cgcggagcgc ccctcgcact tgtagcggaa gcgcatgccc cgctgcttgg
3301 gctgctcaat gatctccaca taggggccag aggcctgggc tggctctgcc gggaagatga
3361 gggggaacag ttcgtccatg gccggggtcc cctggtcgac acccgggaat tccggcgata
3421 cagtcaactg tctttgacct ttgttactac tctcttccga tgatgatgtc gcacttattc
3481 tatgctgtct caatgttaga ggcatatcag tctccactga agccaatcta tctgtgacgg
3541 catctttatt cacattatct tgtacaaata atcctgttaa caatgctttt atatcctgta
3601 aagaatccat tttcaaaatc atgtcaaggt cttctcgagg aaaaatcagt agaaatagct
3661 gttccagtct ttctagcctt gattccactt ctgtcagatg tgccctagtc agcggagacc
3721 ttttggtttt gggagagtag cgacactccc agttgttctt cagacacttg gcgcacttcg
3781 gtttttcttt ggagcacttg agctttttaa gtcggcaaat atcgcatgct tgttcgatag
3841 aagacagtag cttcagatct ggctgctggg agtccatcag tgaatatcaa ctctggcaga
3901 ggagccgctc gcccgccacc gtccgcagtt cccgccgcag ccgagataaa caacttagct
3961 tgtgaacgca gaaggagcag gaggtaccca gcttggaggt gcacaccaat gtggtgaatg
4021 gtcaaatggc gtttattgta tcgagctagg cacttaaata caatatctct gcaatgcgga
4081 attcagtggt tcgtccaatc catgtcagac ctgtctgttg ccttcctaat aaggcacgat
4141 cgtaccacct tacttccacc aatcggcatg cacggtgctt tttctctcct tgtaaggcat
4201 gttgctaact catcgttacc atgttgcaag actacaagtg tattgcataa gactacattt
4261 ccccctccct atgcaaaagc gaaactacta tatcctgagg ggactcctaa ccgcgtacaa
4321 ccgaagcccc gcttttcgcc taaacacacc ctagtcccct cagatacgcg tatatctggc
4381 ccgtacatcg cgaagcagcg caaaacgcct aaccctaagc agattcttca tgcaattgtc
4441 ggtcaagcct tgccttgttg tagcttaaat tttgctcgcg cactactcag cgacctccaa
4501 cacacaagca gggagcagat actggcttaa ctatgcggca tcagagcaga ttgtactgag
4561 agtgcaccat atggctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
4621 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
4681 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
4741 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
4801 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
4861 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
4921 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt
4981 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
5041 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
5101 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
5161 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
5221 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
5281 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
5341 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
5401 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
5461 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
5521 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
5581 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
5641 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
5701 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
5761 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
5821 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
5881 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
5941 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
6001 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
6061 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
6121 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
6181 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca
6241 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
6301 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
6361 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
6421 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
6481 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg
6541 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
6601 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
6661 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
6721 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
6781 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
6841 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
6901 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
6961 tcacgaggcc ctttcgtctt caagaa
//