LOCUS LT727138 7173 bp DNA circular SYN 06-FEB-2017 DEFINITION Insect expression vector pBlueBacmsGCA1C239A-C244A, complete sequence. ACCESSION LT727138 VERSION LT727138.1 KEYWORDS . SOURCE Insect expression vector pBlueBacmsGCA1C239A-C244A ORGANISM Insect expression vector pBlueBacmsGCA1C239A-C244A other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 7173) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..7173 /organism="Insect expression vector pBlueBacmsGCA1C239A-C244A" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 4642." /db_xref="taxon:1944737" regulatory 7..95 /note="AcNPV-PH promoter" /regulatory_class="promoter" CDS 213..2288 /transl_table=11 /note="mGucy1a3 mutant" /protein_id="SJL87570.1" /translation="MFCRKFKDLKITGECPFSLLAPGQVPKEPTEEVAGGSEGCQATL PICQYFPEKNAEGSLPQRKTSRNRVYLHTLAESICKLIFPECERLNLALQRTLAKHKI EENRKSSEKEDLEKIIAEEAIAAGAPVEALKDSLGEELFKICYEEDEHILGVVGGTLK DFLNSFSTLLKQSSHCQEAERRGRLEDASILCLDKDQDFLNVYYFFPKRTTALLLPGI IKAAARILYESHVEVSLMPPAFRSDATEFVNQPYLLYSVHVKSTKPSLSPGKPQSSLV IPASLFCKTFPFHFMLDRDLAILQLGNGIRRLVNKRDFQGKPNFEEFFEILTPKINQT FSGIMTMLNMQFVIRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLED FTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEHAHQALEEEKKRT VDLLCSIFPSEVAQQLWQGQIVQAKKFSEVTMLFSDIVGFTAICSQCSPLQVITMLNA LYTRFDQQCGELDVYKVETIGDAYCVAGGLHRESDTHAVQIALMALKMMELSNEVMSP HGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYR LLKDCPGFVFTPRSREELPPNFPSDIPGICHFLDAYHHQGPNSKPWFQDKDVEDGNAN FLGKASGVD" misc_feature 927..929 /note="A239" misc_feature 942..944 /note="A244" misc_feature 2342..2383 /note="V5 epitope" misc_feature 2393..2410 /note="His-tag" regulatory 2435..2509 /note="SV40 polyA early" /regulatory_class="terminator" regulatory complement(2435..2509) /note="SV40 polyA late" /regulatory_class="terminator" CDS complement(2697..>3575) /codon_start=1 /transl_table=11 /note="AcNPV ORF 1629 fragment" /protein_id="SJL87571.1" /translation="PETSTIVEVPTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPP MVDLSSAPPPPPLVDLPSEMLPPPAPSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSS TTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNRPNKANTPDVKYVQALFNV FTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKARLLLQDLASFVALSENPL DSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIFALTYHPKDYKFNELLK YVQQLSVNQQRTESSA" CDS 3963..4823 /transl_table=11 /note="ampicillin resistance" /protein_id="SJL87572.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5364..5758 /note="pMB1 ori" CDS complement(<5759..6871) /transl_table=11 /note="lacZ fragment" /protein_id="SJL87573.1" /translation="MIDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPS QQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYP ITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQD SRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQI SDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIE NGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQD" regulatory complement(6872..7146) /note="AcNPV-ETL promoter" /regulatory_class="promoter" BASE COUNT 1806 a 1758 c 1779 g 1830 t ORIGIN 1 gatatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt 61 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca 121 ccatcgggcg tgctagcgga tccgagctcg agatctgcag ctggtacccg ggtctagaat 181 tgcccttgcc actgccaagc tccaggaaca ccatgttctg caggaagttc aaggatctca 241 agatcacagg ggagtgtcct ttctccttac tggcccctgg tcaggttcct aaggagccaa 301 cagaggaggt ggctggaggc tctgagggct gccaggctac tctgcccatc tgccagtact 361 ttcctgagaa gaatgcagaa gggagtctcc cccaaagaaa gacaagccgc aacagagtct 421 acctgcacac cctggcagag agtatttgca agctcatctt cccagagtgt gagcgactga 481 accttgcact tcagagaacc ttggcaaagc ataaaataga agaaaacagg aaatcttcag 541 aaaaagaaga ccttgaaaaa ataatcgcag aagaagcaat tgcagcaggt gccccagtgg 601 aggcgctcaa agactctctg ggcgaggagc tgttcaagat ctgctatgag gaagatgagc 661 acattttggg cgtggttggc ggcaccctga aggactttct aaatagcttc agcacgctcc 721 tcaagcagag cagccactgc caagaggcgg agaggcgggg acgactggaa gatgcctcca 781 tcttatgcct ggacaaggac caggactttc taaatgttta ctacttcttc ccgaagagaa 841 ccacagccct gcttctccct ggtatcatta aagcggctgc tcgcatactg tacgaaagcc 901 acgtggaggt gtccctgatg cctcccgcct tccgaagtga cgctaccgag tttgtgaacc 961 agccctattt gctctactcc gttcatgtga agagcaccaa gccgtccctg tccccaggca 1021 agccccagtc ctcgctagtg atccccgctt cgctcttctg caagactttc ccgttccatt 1081 tcatgctgga ccgagacctg gccatcctgc agctgggtaa cggcatcaga aggctggtga 1141 acaagaggga cttccaaggg aagcccaact ttgaagagtt ctttgaaatt ctaactccca 1201 aaatcaacca gacatttagt ggcatcatga caatgttgaa catgcagttt gtcatccggg 1261 tgaggagatg ggataactcg gtgaagaaat cgtcaagggt tatggatctc aaaggtcaaa 1321 tgatctacat cgttgaatcc agtgccatct tgttcttagg gtcaccatgt gtggacaggc 1381 tggaagattt cacaggacgg gggctctatc tgtccgacat cccaattcat aacgccctga 1441 gggatgttgt cttgataggg gagcaggcac gggctcaaga tggcctcaag aagaggttgg 1501 ggaagctgaa ggcaaccctg gagcatgccc accaagccct ggaggaagag aagaagagga 1561 cagtggatct gctgtgctct atcttcccct ctgaggttgc tcagcagctg tggcaaggac 1621 aaattgtgca agccaagaaa ttcagcgagg tcaccatgct tttctcagat atcgtagggt 1681 tcactgctat ctgctctcag tgttcacctc tgcaggtcat cacgatgctc aacgctctct 1741 acactcgctt tgaccagcag tgtggagagc tggatgtcta caaggtggag accatcgggg 1801 atgcatattg tgtggcaggt ggattgcaca gagagagtga cacccatgct gtccagatag 1861 cactgatggc cctgaagatg atggagctct ccaatgaggt catgtctccc cacggagaac 1921 ctatcaagat gcgaattgga ctacattctg gatcagtgtt tgctggagtt gtcggagtga 1981 agatgccccg gtattgcctg tttggaaaca atgtcactct ggctaacaaa tttgaatcct 2041 gcagtgtgcc tcggaaaatc aatgtcagcc ccaccacata caggttactc aaagactgtc 2101 ctggctttgt gttcaccccg agatcaaggg aggagcttcc accaaacttc cctagtgaca 2161 ttcctgggat ctgtcacttt ctggatgctt atcaccatca aggacctaat tccaaaccat 2221 ggttccagga taaagatgtg gaagatggaa acgccaactt cttaggcaaa gcgtcagggg 2281 tagattagtg agccacatgc tcttatgttt gatgcctaag ggcaattcga agcttaggcc 2341 tggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg gtcatcatca 2401 ccatcaccat tgacgtctct agcttggagt cgacaacttg tttattgcag cttataatgg 2461 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 2521 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat ctctcgactc tgctgaagag 2581 gaggaaattc tccttgaagt ttccctggtg ttcaaagtaa aggagtttgc accagacgca 2641 cctctgttca ctggtccggc gtattaaaac acgatacatt gttattagta catttattaa 2701 gcgctagatt ctgtgcgttg ttgatttaca gacaattgtt gtacgtattt taataattca 2761 ttaaatttat aatctttagg gtggtatgtt agagcgaaaa tcaaatgatt ttcagcgtct 2821 ttatatctga atttaaatat taaatcctca atagatttgt aaaataggtt tcgattagtt 2881 tcaaacaagg gttgtttttc cgaaccgatg gctggactat ctaatggatt ttcgctcaac 2941 gccacaaaac ttgccaaatc ttgtagcagc aatctagctt tgtcgatatt cgtttgtgtt 3001 ttgttttgta ataaaggttc gacgtcgttc aaaatattat gcgcttttgt atttctttca 3061 tcactgtcgt tagtgtacaa ttgactcgac gtaaacacgt taaataaagc ctggacatat 3121 ttaacatcgg gcgtgttagc tttattaggc cgattatcgt cgtcgtccca accctcgtcg 3181 ttagaagttg cttccgaaga cgattttgcc atagccacac gacgcctatt aattgtgtcg 3241 gctaacacgt ccgcgatcaa atttgtagtt gagctttttg gaattatttc tgattgcggg 3301 cgtttttggg cgggtttcaa tctaactgtg cccgatttta attcagacaa cacgttagaa 3361 agcgatggtg caggcggtgg taacatttca gacggcaaat ctactaatgg cggcggtggt 3421 ggagctgatg ataaatctac catcggtgga ggcgcaggcg gggctggcgg cggaggcgga 3481 ggcggaggtg gtggcggtga tgcagacggc ggtttaggct caaatgtctc tttaggcaac 3541 acagtcggca cctcaactat tgtactggtt tcgggcgatg gtgcactctc agtacaatct 3601 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct 3661 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct 3721 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga 3781 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca 3841 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata 3901 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga 3961 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc 4021 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg 4081 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc 4141 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat 4201 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact 4261 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat 4321 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga 4381 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc 4441 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga 4501 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag 4561 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc 4621 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt 4681 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct 4741 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg 4801 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg 4861 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca 4921 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga 4981 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 5041 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 5101 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt 5161 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 5221 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 5281 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 5341 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca 5401 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 5461 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 5521 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 5581 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 5641 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag 5701 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcat 5761 cctgcaccat cgtctgctca tccatgacct gaccatgcag aggatgatgc tcgtgacggt 5821 taacgcctcg aatcagcaac ggcttgccgt tcagcagcag cagaccattt tcaatccgca 5881 cctcgcggaa accgacatcg caggcttctg cttcaatcag cgtgccgtcg gcggtgtgca 5941 gttcaaccac cgcacgatag agattcggga tttcggcgct ccacagtttc gggttttcga 6001 cgttcagacg tagtgtgacg cgatcggcat aaccaccacg ctcatcgata atttcaccgc 6061 cgaaaggcgc ggtgccgctg gcgacctgcg tttcaccctg ccataaagaa actgttaccc 6121 gtaggtagtc acgcaactcg ccgcacatct gaacttcagc ctccagtaca gcgcggctga 6181 aatcatcatt aaagcgagtg gcaacatgga aatcgctgat ttgtgtagtc ggtttatgca 6241 gcaacgagac gtcacggaaa atgccgctca tccgccacat atcctgatct tccagataac 6301 tgccgtcact ccagcgcagc accatcaccg cgaggcggtt ttctccggcg cgtaaaaatg 6361 cgctcaggtc aaattcagac ggcaaacgac tgtcctggcc gtaaccgacc cagcgcccgt 6421 tgcaccacag atgaaacgcc gagttaacgc catcaaaaat aattcgcgtc tggccttcct 6481 gtagccagct ttcatcaaca ttaaatgtga gcgagtaaca acccgtcgga ttctccgtgg 6541 gaacaaacgg cggattgacc gtaatgggat aggtcacgtt ggtgtagatg ggcgcatcgt 6601 aaccgtgcat ctgccagttt gaggggacga cgacagtatc ggcctcagga agatcgcact 6661 ccagccagct ttccggcacc gcttctggtg ccggaaacca ggcaaagcgc cattcgccat 6721 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc 6781 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt 6841 cacgacgttg taaaacgacg ggatctatca tttttagcag tgattctaat tgcagctgct 6901 ctttgataca actaatttta cgacgacgat gcgagctttt attcaaccga gcgtgcatgt 6961 ttgcaatcgt gcaagcgtta tcaatttttc attatcgtat tgttgcacat caacaggctg 7021 gacaccacgt tgaactcgcc gcagttttgc ggcaagttgg acccgccgcg catccaatgc 7081 aaactttccg acattctgtt gcctacgaac gattgattct ttgtccattg atcgaagcga 7141 gtgccttcga ctttttcgtg tccagtgtgg ctt //