LOCUS LT727135 5572 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGShBcl-2, complete sequence.
ACCESSION LT727135
VERSION LT727135.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGShBcl-2
ORGANISM Mammalian expression vector pCAGGShBcl-2
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5572)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5572
/organism="Mammalian expression vector pCAGGShBcl-2"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4633."
/db_xref="taxon:1944870"
CDS 36..755
/transl_table=11
/note="hBCL2"
/protein_id="SJL87561.1"
/translation="MAHAGRTGYDNREIVMKYIHYKLSQRGYEWDAGDVGAAPPGAAP
APGIFSSQPGHTPHPAASRDPVARTSPLQTPAAPGAAAGPALSPVPPVVHLTLRQAGD
DFSRRYRRDFAEMSSQLHLTPFTARGRFATVVEELFRDGVNWGRIVAFFEFGGVMCVE
SVNREMSPLVDNIALWMTEYLNRHLHTWIQDNGGWDAFVELYGPSMRPLFDFSWLSLK
TLLSLALVGACITLGAYLGHK"
regulatory 862..953
/note="3' UTR of rabbit beta-globin, incl. polyA"
/regulatory_class="terminator"
misc_feature 954..1310
/note="3' flanking region of rabbit beta-globin"
regulatory complement(1388..1470)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1612..1687
/note="SV40 ori"
regulatory 1718..1792
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(1718..1792)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(1907..2308)
/note="pMB1 ori"
CDS complement(2849..3709)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87562.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3845..4222
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 4228..5569
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4597..5521
/note="intron AG promoter"
BASE COUNT 1125 a 1527 c 1587 g 1333 t
ORIGIN
1 ttcggcttgc ccccgttgct tttcctctgg gaaggatggc gcacgctggg agaacggggt
61 acgataaccg ggagatagtg atgaagtaca tccattataa gctgtcgcag aggggctacg
121 agtgggatgc gggagatgtg ggcgccgcgc ccccgggggc cgcccccgca ccgggcatct
181 tctcctccca gcccgggcac acgccccatc cagccgcatc ccgcgacccg gtcgccagga
241 cctcgccgct gcagaccccg gctgcccccg gcgccgccgc ggggcctgcg ctcagcccgg
301 tgccacctgt ggtccacctg accctccgcc aggccggcga cgacttctcc cgccgctacc
361 gccgcgactt cgccgagatg tccagccagc tgcacctgac gcccttcacc gcgcggggac
421 gctttgccac ggtggtggag gagctcttca gggacggggt gaactggggg aggattgtgg
481 ccttctttga gttcggtggg gtcatgtgtg tggagagcgt caaccgggag atgtcgcccc
541 tggtggacaa catcgccctg tggatgactg agtacctgaa ccggcacctg cacacctgga
601 tccaggataa cggaggctgg gatgcctttg tggaactgta cggccccagc atgcggcctc
661 tgtttgattt ctcctggctg tctctgaaga ctctgctcag tttggccctg gtgggagctt
721 gcatcaccct gggtgcctat ctgggccaca agtgaagtca acatgcctgc cccaaaagcc
781 gaattcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc
841 ctggctcaca aataccactg agatcttttt ccctctgcca aaaattatgg ggacatcatg
901 aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg
961 tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa
1021 catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg
1081 aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc
1141 ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta
1201 tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc
1261 ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc cctcgacctg
1321 cagcccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
1381 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg tgcctaatga
1441 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
1501 tcgtgccagc ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
1561 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
1621 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
1681 gaggcttttt tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa
1741 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
1801 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat
1861 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
1921 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
1981 aaggcggtaa tacggttatc cacagaatca gggataacgc aggaaagaac atgtgagcaa
2041 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
2101 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
2161 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
2221 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
2281 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
2341 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
2401 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
2461 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
2521 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
2581 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
2641 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
2701 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
2761 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
2821 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
2881 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
2941 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
3001 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
3061 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
3121 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
3181 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
3241 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
3301 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
3361 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
3421 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
3481 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
3541 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
3601 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
3661 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
3721 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
3781 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
3841 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
3901 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
3961 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
4021 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
4081 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
4141 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
4201 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
4261 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
4321 gcgatggggg cggggggggg gggggggccc cccccaggcg gggcggggcg gggcgagggg
4381 cggggcgggg cgaggcggaa aggtgcggcg gcagccaatc agagcggcgc gctccgaaag
4441 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg
4501 gcgggagtcg ttgcgcgctg ccttcccccc gtgccccgct ccgccgccgc ctcgcgccgc
4561 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct
4621 cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt
4681 gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg
4741 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag
4801 cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc
4861 gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt
4921 gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg
4981 cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg
5041 cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg
5101 gcggggccgc ctcgggccgg ggagggctcg ggggaagggg cgcggcggcc cccggagcgc
5161 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
5221 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
5281 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
5341 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
5401 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
5461 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
5521 gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aa
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