LOCUS LT727127 7980 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pEF1-2FKBP-link-hFADD-DN, complete
sequence.
ACCESSION LT727127
VERSION LT727127.1
KEYWORDS .
SOURCE Mammalian expression vector pEF1-2FKBP-link-hFADD-DN
ORGANISM Mammalian expression vector pEF1-2FKBP-link-hFADD-DN
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7980)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..7980
/organism="Mammalian expression vector
pEF1-2FKBP-link-hFADD-DN"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4611."
/db_xref="taxon:1944969"
regulatory 508..1651
/note="hEF1 promoter"
/regulatory_class="promoter"
misc_feature 704..1642
/note="intron hEF1 promoter"
regulatory 1668..1684
/note="T7 promoter"
/regulatory_class="promoter"
CDS 1760..>1801
/transl_table=11
/note="v-src myristoylation-targeting sequence"
/protein_id="SJL87524.1"
/translation="MGSSKSKPKDPSQR"
CDS <1808..>2128
/codon_start=1
/transl_table=11
/note="hFKBP1A"
/protein_id="SJL87525.1"
/translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVE
LLKLE"
CDS <2135..>2455
/codon_start=1
/transl_table=11
/note="hFKBP1A"
/protein_id="SJL87526.1"
/translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVE
LLKLE"
misc_feature 2462..2479
/note="GSGGGS linker"
CDS <2486..>2872
/codon_start=1
/transl_table=11
/note="hFADD-DN"
/protein_id="SJL87527.1"
/translation="DDFEAGAAAGAAPGEEDLCAAFNVICDNVGKDWRRLARQLKVSD
TKIDSIEDRYPRNLTERVRESLRIWKNTEKENATVAHLVGALRSCQMNLVADLVQEVQ
QARDLQNRSGAMSPMSWNSDASTSEAS"
misc_feature 2879..2905
/note="HA epitope"
misc_feature 3611..3652
/note="V5 epitope"
misc_feature 3662..3679
/note="His-tag"
regulatory 3708..3932
/note="BGH polyA"
/regulatory_class="terminator"
rep_origin 3978..4406
/note="f1 ori"
regulatory 4411..4692
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 4649..4724
/note="SV40 ori"
CDS 4816..5610
/transl_table=11
/note="Tn5 neomycin resistance"
/protein_id="SJL87528.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
regulatory 5786..5860
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(5786..5860)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory complement(5999..6082)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(6174..6565)
/note="pMB1 ori"
CDS complement(7106..7966)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87529.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1782 a 2028 c 2147 g 2023 t
ORIGIN
1 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
61 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
121 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
181 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
241 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
301 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
361 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga
421 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg
481 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
541 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
601 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
661 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
721 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
781 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
841 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
901 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
961 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
1021 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
1081 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
1141 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
1201 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
1261 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
1321 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
1381 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
1441 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
1501 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
1561 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
1621 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac
1681 tatagggaga cccaagctgg ctaggtaagc ttggtaccga gctcggatcc actagtccag
1741 tgtggtggaa ttcgcgcgta tggggagtag caagagcaag cctaaggacc ccagccagcg
1801 ctctagagga gtgcaggtgg aaaccatctc cccgggagac gggcgcacct tccccaagcg
1861 cggccagacc tgcgtggtgc actacaccgg gatgcttgaa gatggaaaga aatttgattc
1921 ctcccgggac agaaacaagc cctttaagtt tatgctaggc aagcaggagg tgatccgagg
1981 ctgggaagaa ggggttgccc agatgagtgt gggtcagaga gccaaactga ctatatctcc
2041 agattatgcc tatggtgcca ctgggcaccc aggcatcatc ccaccacatg ccactctcgt
2101 cttcgatgtg gagcttctaa aactggaagt cgagggcgtg caggtggaaa ccatctcccc
2161 aggagacggg cgcaccttcc ccaagcgcgg ccagacctgc gtggtgcact acaccgggat
2221 gcttgaagat ggaaagaaat ttgattcctc ccgggacaga aacaagccct ttaagtttat
2281 gctaggcaag caggaggtga tccgaggctg ggaagaaggg gttgcccaga tgagtgtggg
2341 tcagagagcc aaactgacta tatctccaga ttatgcctat ggtgccactg ggcacccagg
2401 catcatccca ccacatgcca ctctcgtctt cgatgtggag cttctaaaac tggaaactag
2461 agggtctgga ggcggatcct ctagtgacga cttcgaggcg ggggcggcgg ccggggccgc
2521 gcctggggaa gaagacctgt gtgcagcatt taacgtcata tgtgataatg tggggaaaga
2581 ttggagaagg ctggctcgtc agctcaaagt ctcagacacc aagatcgaca gcatcgagga
2641 cagatacccc cgcaacctga cagagcgtgt gcgggagtca ctgagaatct ggaagaacac
2701 agagaaggag aacgcaacag tggcccacct ggtgggggct ctcaggtcct gccagatgaa
2761 cctggtggct gacctggtac aagaggttca gcaggcccgt gacctccaga acaggagtgg
2821 ggccatgtcc ccgatgtcat ggaactcaga cgcatctacc tccgaagcgt cctctagtta
2881 tccgtacgac gtaccagact acgcataaga aaagtgagga tcctgagaac ttcagggtga
2941 gtttggggac ccttgattgt tctttctttt tcgctattgt aaaattcatg ttatatggag
3001 ggggcaaagt tttcagggtg ttgtttagaa tgggaagatg tcccttgtat caccatggac
3061 cctcatgata attttgtttc tttcactttc tactctgttg acaaccattg tctcctctta
3121 ttttcttttc attttctgta actttttcgt taaactttag cttgcatttg taacgaattt
3181 ttaaattcac ttttgtttat ttgtcagatt gtaagtactt tctctaatca cttttttttc
3241 aaggcaatca gggtatatta tattgtactt cagcacagtt ttagagaaca attgttataa
3301 ttaaatgata aggtagaata tttctgcata taaattctgg ctggcgtgga aatattctta
3361 ttggtagaaa caactacatc ctggtcatca tcctgccttt ctctttatgg ttacaatgat
3421 atacactgtt tgagatgagg ataaaatact ctgagtccaa accgggcccc tctgctaacc
3481 atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt gttgtgctgt
3541 ctcatcattt tggcaaagaa ttctgcagat atccagcaca gtggcggccg ctcgaggtca
3601 cccattcgaa ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg
3661 tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg tgccttctag
3721 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
3781 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
3841 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
3901 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
3961 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
4021 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
4081 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
4141 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
4201 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
4261 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
4321 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
4381 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
4441 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
4501 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
4561 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
4621 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
4681 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
4741 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca agagacagga
4801 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
4861 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
4921 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
4981 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
5041 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
5101 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
5161 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
5221 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
5281 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
5341 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
5401 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
5461 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
5521 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
5581 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgcg aaatgaccga
5641 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
5701 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
5761 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
5821 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
5881 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
5941 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
6001 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
6061 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
6121 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
6181 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
6241 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
6301 gtgagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
6361 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
6421 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
6481 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
6541 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
6601 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
6661 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
6721 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
6781 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
6841 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
6901 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
6961 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
7021 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
7081 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
7141 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
7201 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
7261 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
7321 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
7381 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
7441 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
7501 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
7561 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
7621 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
7681 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
7741 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
7801 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
7861 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
7921 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
//