LOCUS LT727125 3759 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pUHD-Etag-mBAX, complete sequence.
ACCESSION LT727125
VERSION LT727125.1
KEYWORDS .
SOURCE Vector pUHD-Etag-mBAX
ORGANISM Vector pUHD-Etag-mBAX
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 3759)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..3759
/organism="Vector pUHD-Etag-mBAX"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4609."
/db_xref="taxon:1945436"
regulatory 2..369
/note="hCMVT promoter"
/regulatory_class="promoter"
misc_feature 462..500
/note="E-tag"
CDS 510..1088
/transl_table=11
/note="mBAX"
/protein_id="SJL87516.1"
/translation="MDGSGEQLGSGGPTSSEQIMKTGAFLLQGFIQDRAGRMAGETPE
LTLEQPPQDASTKKLSECLRRIGDELDSNMELQRMIADVDTDSPREVFFRVAADMFAD
GNFNWGRVVALFYFASKLVLKALCTKVPELIRTIMGWTLDFLRERLLVWIQDQGGWEG
LLSYFGTPTWQTVTIFVAGVLTASLTIWKKMG"
regulatory complement(1156..1252)
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory 1156..1252
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(1756..2158)
/note="pMB1 ori"
CDS complement(2699..3559)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87517.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 958 a 946 c 958 g 897 t
ORIGIN
1 ctcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
61 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
121 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
181 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
241 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
301 ctcggtaccc gggtcgagta ggcgtgtacg gtgggaggcc tatataagca gagctcgttt
361 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca
421 ccgggaccga tccagcctcc gcggccccga attccaccat gggtgcgccg gtgccgtatc
481 cggacccgct ggaaccgcgt gcggccgcta tggacgggtc cggggagcag cttgggagcg
541 gcgggcccac cagctctgaa cagatcatga agacaggggc ctttttgcta cagggtttca
601 tccaggatcg agcagggagg atggctgggg agacacctga gctgaccttg gagcagccgc
661 cccaggatgc gtccaccaag aagctgagcg agtgtctccg gcgaattgga gatgaactgg
721 atagcaatat ggagctgcag aggatgattg ctgacgtgga cacggactcc ccccgagagg
781 tcttcttccg ggtggcagct gacatgtttg ctgatggcaa cttcaactgg ggccgcgtgg
841 ttgccctctt ctactttgct agcaaactgg tgctcaaggc cctgtgcact aaagtgcccg
901 agctgatcag aaccatcatg ggctggacac tggacttcct ccgtgagcgg ctgcttgtct
961 ggatccaaga ccagggtggc tgggaaggcc tcctctccta cttcgggacc cccacatggc
1021 agacagtgac catctttgtg gctggagtcc tcaccgcctc gctcaccatc tggaagaaga
1081 tgggctgatc tagaggatcc agacatgata agatacattg atgagtttgg acaaaccaca
1141 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
1201 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
1261 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
1321 atggctgatt atgatcctgc aagcctcgtc gtctggccgg accacgctat ctgtgcaagg
1381 tccccggacg cgcgctccat gagcagagcg cccgccgccg aggcaagact cgggcggcgc
1441 cctgcccgtc ccaccaggtc aacaggcggt aaccggcctc ttcatcggga atgcgcgcga
1501 ccttcagcat cgccggcatg tcccctggcg gacgggaagt atcagctcga ccaagcttgg
1561 cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg
1621 ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat
1681 gtacctataa ccagaccgtt cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
1741 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
1801 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
1861 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
1921 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
1981 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
2041 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
2101 cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt
2161 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
2221 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
2281 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
2341 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
2401 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
2461 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
2521 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
2581 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
2641 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
2701 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
2761 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
2821 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
2881 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
2941 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
3001 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
3061 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
3121 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
3181 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
3241 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
3301 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
3361 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
3421 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
3481 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
3541 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
3601 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
3661 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
3721 taacctataa aaataggcgt atcacgaggc cctttcgtc
//