LOCUS LT727111 7087 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pEF1-AVPS-hOMI, complete sequence.
ACCESSION LT727111
VERSION LT727111.1
KEYWORDS .
SOURCE Mammalian expression vector pEF1-AVPS-hOMI
ORGANISM Mammalian expression vector pEF1-AVPS-hOMI
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7087)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..7087
/organism="Mammalian expression vector pEF1-AVPS-hOMI"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4582."
/db_xref="taxon:1944971"
regulatory 508..1651
/note="hEF1 promoter"
/regulatory_class="promoter"
misc_feature 704..1642
/note="intron hEF1 promoter"
regulatory 1668..1684
/note="T7 promoter"
/regulatory_class="promoter"
CDS 1719..2699
/transl_table=11
/note="mature hOMI"
/protein_id="SJL87462.1"
/translation="MAVPSPPPASPRSQYNFIADVVEKTAPAVVYIEILDRHPFLGRE
VPISNGSGFVVAADGLIVTNAHVVADRRRVRVRLLSGDTYEAVVTAVDPVADIATLRI
QTKEPLPTLPLGRSADVRQGEFVVAMGSPFALQNTITSGIVSSAQRPARDLGLPQTNV
EYIQTDAAIDFGNSGGPLVNLDGEVIGVNTMKVTAGISFAIPSDRLREFLHRGEKKNS
SSGISGSQRRYIGVMMLTLSPSILAELQLREPSFPDVQHGVLIHKVILGSPAHRAGLR
PGDVILAIGEQMVQNAEDVYEAVRTQSQLAVQIRRGRETLTLYVTPEVTE"
misc_feature 2718..2759
/note="V5 epitope"
misc_feature 2769..2786
/note="His-tag"
regulatory 2815..3039
/note="bovine growth hormone polyA"
/regulatory_class="terminator"
rep_origin 3085..3513
/note="f1 ori"
regulatory 3518..3799
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 3756..3831
/note="SV40 ori"
CDS 3923..4717
/transl_table=11
/note="neomycin"
/protein_id="SJL87463.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
regulatory 4893..4967
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(4893..4967)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory complement(5106..5189)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(5281..5672)
/note="pMB1 ori"
CDS complement(6213..7073)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87464.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1525 a 1858 c 1936 g 1768 t
ORIGIN
1 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
61 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
121 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
181 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
241 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
301 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
361 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga
421 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg
481 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
541 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
601 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
661 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
721 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
781 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
841 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
901 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
961 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
1021 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
1081 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
1141 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
1201 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
1261 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
1321 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
1381 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
1441 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
1501 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
1561 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
1621 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac
1681 tatagggaga cccaagctgg ctaggtaagc ttggtaccat ggccgtccct agcccgccgc
1741 ccgcttctcc ccggagtcag tacaacttca tcgcagatgt ggtggagaag acagcacctg
1801 ccgtggtcta tatcgagatc ctggaccggc accctttctt gggccgcgag gtccctatct
1861 cgaacggctc aggattcgtg gtggctgccg atgggctcat tgtcaccaac gcccatgtgg
1921 tggctgatcg gcgcagagtc cgtgtgagac tgctaagcgg cgacacgtat gaggccgtgg
1981 tcacagctgt ggatcccgtg gcagacatcg caacgctgag gattcagact aaggagcctc
2041 tccccacgct gcctctggga cgctcagctg atgtccggca aggggagttt gttgttgcca
2101 tgggaagtcc ctttgcactg cagaacacga tcacatccgg cattgttagc tctgctcagc
2161 gtccagccag agacctggga ctcccccaaa ccaatgtgga atacattcaa actgatgcag
2221 ctattgattt tggaaactct ggaggtcccc tggttaacct ggatggggag gtgattggag
2281 tgaacaccat gaaggtcaca gctggaatct cctttgccat cccttctgat cgtcttcgag
2341 agtttctgca tcgtggggaa aagaagaatt cctcctccgg aatcagtggg tcccagcggc
2401 gctacattgg ggtgatgatg ctgaccctga gtcccagcat ccttgctgaa ctacagcttc
2461 gagaaccaag ctttcccgat gttcagcatg gtgtactcat ccataaagtc atcctgggct
2521 cccctgcaca ccgggctggt ctgcggcctg gtgatgtgat tttggccatt ggggagcaga
2581 tggtacaaaa tgctgaagat gtttatgaag ctgttcgaac ccaatcccag ttggcagtgc
2641 agatccggcg gggacgagaa acactgacct tatatgtgac ccctgaggtc acagaatgat
2701 ctagagggcc cttcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc
2761 gtaccggtca tcatcaccat caccattgag tttaaacccg ctgatcagcc tcgactgtgc
2821 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
2881 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
2941 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
3001 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
3061 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
3121 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
3181 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
3241 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
3301 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
3361 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
3421 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
3481 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
3541 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
3601 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
3661 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
3721 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
3781 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
3841 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga
3901 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
3961 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
4021 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
4081 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
4141 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
4201 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
4261 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
4321 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
4381 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
4441 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
4501 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
4561 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
4621 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
4681 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgcgaaa
4741 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
4801 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
4861 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
4921 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
4981 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
5041 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
5101 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
5161 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
5221 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
5281 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
5341 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
5401 agaacatgtg agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
5461 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
5521 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
5581 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
5641 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
5701 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
5761 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
5821 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
5881 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
5941 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
6001 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
6061 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
6121 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
6181 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
6241 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
6301 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
6361 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
6421 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
6481 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
6541 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
6601 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
6661 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
6721 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
6781 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
6841 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
6901 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
6961 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
7021 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
7081 ctttttc
//