LOCUS LT727109 7483 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pEF1-hOMI, complete sequence.
ACCESSION LT727109
VERSION LT727109.1
KEYWORDS .
SOURCE Mammalian expression vector pEF1-hOMI
ORGANISM Mammalian expression vector pEF1-hOMI
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7483)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..7483
/organism="Mammalian expression vector pEF1-hOMI"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4580."
/db_xref="taxon:1944978"
regulatory 508..1651
/note="hEF1 promoter"
/regulatory_class="promoter"
misc_feature 704..1642
/note="intron hEF1 promoter"
regulatory 1668..1684
/note="T7 promoter"
/regulatory_class="promoter"
CDS 1719..3095
/transl_table=11
/note="hOMI"
/protein_id="SJL87456.1"
/translation="MAAPRAGRGAGWSLRAWRALGGIRWGRRPRLTPDLRALLTSGTS
DPRARVTYGTPSLWARLSVGVTEPRACLTSGTPGPRAQLTAVTPDTRTREASENSGTR
SRAWLAVALGAGGAVLLLLWGGGRGPPAVLAAVPSPPPASPRSQYNFIADVVEKTAPA
VVYIEILDRHPFLGREVPISNGSGFVVAADGLIVTNAHVVADRRRVRVRLLSGDTYEA
VVTAVDPVADIATLRIQTKEPLPTLPLGRSADVRQGEFVVAMGSPFALQNTITSGIVS
SAQRPARDLGLPQTNVEYIQTDAAIDFGNSGGPLVNLDGEVIGVNTMKVTAGISFAIP
SDRLREFLHRGEKKNSSSGISGSQRRYIGVMMLTLSPSILAELQLREPSFPDVQHGVL
IHKVILGSPAHRAGLRPGDVILAIGEQMVQNAEDVYEAVRTQSQLAVQIRRGRETLTL
YVTPEVTE"
misc_feature 3114..3155
/note="V5 epitope"
misc_feature 3165..3182
/note="His-tag"
regulatory 3211..3435
/note="bovine growth hormone polyA"
/regulatory_class="terminator"
rep_origin 3481..3909
/note="f1 ori"
regulatory 3914..4195
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 4152..4227
/note="SV40 ori"
CDS 4319..5113
/transl_table=11
/note="neomycin"
/protein_id="SJL87457.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
regulatory 5289..5363
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(5289..5363)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory complement(5502..5585)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(5677..6068)
/note="pMB1 ori"
CDS complement(6609..7469)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87458.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1570 a 1981 c 2093 g 1839 t
ORIGIN
1 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
61 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
121 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
181 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
241 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
301 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
361 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga
421 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg
481 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
541 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
601 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
661 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
721 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
781 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
841 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
901 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
961 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
1021 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
1081 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
1141 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
1201 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
1261 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
1321 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
1381 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
1441 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
1501 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
1561 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
1621 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac
1681 tatagggaga cccaagctgg ctaggtaagc ttggtaccat ggctgcgccg agggcggggc
1741 ggggtgcagg ctggagcctt cgggcatggc gggctttggg gggcattcgc tgggggagga
1801 gaccccgttt gacccctgac ctccgggccc tgctgacgtc aggaacttct gacccccggg
1861 cccgagtgac ttatgggacc cccagtctct gggcccggtt gtctgttggg gtcactgaac
1921 cccgagcatg cctgacgtct gggaccccgg gtccccgggc acaactgact gcggtgaccc
1981 cagataccag gacccgggag gcctcagaga actctggaac ccgttcgcgc gcgtggctgg
2041 cggtggcgct gggcgctggg ggggcagtgc tgttgttgtt gtggggcggg ggtcggggtc
2101 ctccggccgt cctcgccgcc gtccctagcc cgccgcccgc ttctccccgg agtcagtaca
2161 acttcatcgc agatgtggtg gagaagacag cacctgccgt ggtctatatc gagatcctgg
2221 accggcaccc tttcttgggc cgcgaggtcc ctatctcgaa cggctcagga ttcgtggtgg
2281 ctgccgatgg gctcattgtc accaacgccc atgtggtggc tgatcggcgc agagtccgtg
2341 tgagactgct aagcggcgac acgtatgagg ccgtggtcac agctgtggat cccgtggcag
2401 acatcgcaac gctgaggatt cagactaagg agcctctccc cacgctgcct ctgggacgct
2461 cagctgatgt ccggcaaggg gagtttgttg ttgccatggg aagtcccttt gcactgcaga
2521 acacgatcac atccggcatt gttagctctg ctcagcgtcc agccagagac ctgggactcc
2581 cccaaaccaa tgtggaatac attcaaactg atgcagctat tgattttgga aactctggag
2641 gtcccctggt taacctggat ggggaggtga ttggagtgaa caccatgaag gtcacagctg
2701 gaatctcctt tgccatccct tctgatcgtc ttcgagagtt tctgcatcgt ggggaaaaga
2761 agaattcctc ctccggaatc agtgggtccc agcggcgcta cattggggtg atgatgctga
2821 ccctgagtcc cagcatcctt gctgaactac agcttcgaga accaagcttt cccgatgttc
2881 agcatggtgt actcatccat aaagtcatcc tgggctcccc tgcacaccgg gctggtctgc
2941 ggcctggtga tgtgattttg gccattgggg agcagatggt acaaaatgct gaagatgttt
3001 atgaagctgt tcgaacccaa tcccagttgg cagtgcagat ccggcgggga cgagaaacac
3061 tgaccttata tgtgacccct gaggtcacag aatgatctag agggcccttc gaaggtaagc
3121 ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac cggtcatcat caccatcacc
3181 attgagttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
3241 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
3301 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
3361 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
3421 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
3481 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
3541 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
3601 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
3661 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
3721 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
3781 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
3841 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
3901 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
3961 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
4021 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
4081 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
4141 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
4201 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
4261 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat
4321 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
4381 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
4441 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
4501 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
4561 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
4621 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
4681 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
4741 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
4801 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
4861 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
4921 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
4981 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
5041 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
5101 cgagttcttc tgagcgggac tctggggttc gcgaaatgac cgaccaagcg acgcccaacc
5161 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
5221 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
5281 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
5341 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
5401 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
5461 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
5521 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
5581 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
5641 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
5701 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
5761 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagg
5821 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
5881 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
5941 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
6001 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
6061 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
6121 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
6181 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
6241 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
6301 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
6361 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
6421 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
6481 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
6541 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
6601 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
6661 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
6721 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
6781 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
6841 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
6901 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
6961 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
7021 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
7081 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
7141 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
7201 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
7261 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
7321 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
7381 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
7441 agggcgacac ggaaatgttg aatactcata ctcttccttt ttc
//