LOCUS LT727107 9788 bp DNA circular SYN 06-FEB-2017
DEFINITION Yeast expression vector pAS2-hOMI-S306A, complete sequence.
ACCESSION LT727107
VERSION LT727107.1
KEYWORDS .
SOURCE Yeast expression vector pAS2-hOMI-S306A
ORGANISM Yeast expression vector pAS2-hOMI-S306A
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 9788)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..9788
/organism="Yeast expression vector pAS2-hOMI-S306A"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4578."
/db_xref="taxon:1945456"
rep_origin complement(2..982)
/note="2mu ori"
regulatory complement(1060..1164)
/note="FLP terminator"
/regulatory_class="terminator"
CDS 1884..2558
/transl_table=11
/note="TRP1"
/protein_id="SJL87446.1"
/translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
VKNAKK"
rep_origin complement(2661..3116)
/note="f1 ori"
regulatory complement(3312..3586)
/note="CYC1 terminator"
/regulatory_class="terminator"
CDS complement(3855..4304)
/transl_table=11
/note="CYH2"
/protein_id="SJL87447.1"
/translation="MPSRFTKTRKHRGHVSAGKGRIGKHRKHPGGRGMAGGQHHHRIN
MDKYHPGYFGKVGMRYFHKQQAHFWKPVLNLDKLWTLIPEDKRDQYLKSASKETAPVI
DTLAAGYGKILGKGRIPNVPVIVKARFVSKLAEEKIRAAGGVVELIA"
regulatory 5075..5478
/note="ADH1 promoter"
/regulatory_class="promoter"
CDS 5501..>5941
/transl_table=11
/note="GALbd"
/protein_id="SJL87448.1"
/translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT
KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV
NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
misc_feature 5957..5983
/note="HA epitope"
CDS 5999..7375
/transl_table=11
/note="hOMI mutant"
/protein_id="SJL87449.1"
/translation="MAAPRAGRGAGWSLRAWRALGGIRWGRRPRLTPDLRALLTSGTS
DPRARVTYGTPSLWARLSVGVTEPRACLTSGTPGPRAQLTAVTPDTRTREASENSGTR
SRAWLAVALGAGGAVLLLLWGGGRGPPAVLAAVPSPPPASPRSQYNFIADVVEKTAPA
VVYIEILDRHPFLGREVPISNGSGFVVAADGLIVTNAHVVADRRRVRVRLLSGDTYEA
VVTAVDPVADIATLRIQTKEPLPTLPLGRSADVRQGEFVVAMGSPFALQNTITSGIVS
SAQRPARDLGLPQTNVEYIQTDAAIDFGNAGGPLVNLDGEVIGVNTMKVTAGISFAIP
SDRLREFLHRGEKKNSSSGISGSQRRYIGVMMLTLSPSILAELQLREPSFPDVQHGVL
IHKVILGSPAHRAGLRPGDVILAIGEQMVQNAEDVYEAVRTQSQLAVQIRRGRETLTL
YVTPEVTE"
misc_feature 6914..6919
/note="S306A"
regulatory 7426..7527
/note="ADH1 terminator"
/regulatory_class="terminator"
regulatory complement(7680..7763)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(7855..8257)
/note="pMB1 ori"
CDS complement(8798..9658)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87450.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 2606 a 2151 c 2315 g 2716 t
ORIGIN
1 gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt
61 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt
121 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta
181 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg
241 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag
301 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat
361 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg
421 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact
481 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga
541 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca
601 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac
661 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa
721 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag
781 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag
841 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt
901 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg
961 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt
1021 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca
1081 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt
1141 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc
1201 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact
1261 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc
1321 tatcatttcc tttgatattg gatcatatta agaaaccatt attatcatga cattaaccta
1381 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
1441 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
1501 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
1561 tgcggcatca gagcagattg tactgagagt gcaccataga tcaacgacat tactatatat
1621 ataatatagg aagcatttaa tagacagcat cgtaatatat gtgtactttg cagttatgac
1681 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca
1741 atcttgatcc ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa
1801 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta
1861 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat
1921 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg
1981 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg
2041 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact
2101 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg
2161 gcattgatat cgtccaactg catggagatg agtcgtggca agaataccaa gagttcctcg
2221 gtttgccagt tattaaaaga ctcgtatttc caaaagactg caacatacta ctcagtgcag
2281 cttcacagaa acctcattcg tttattccct tgtttgattc agaagcaggt gggacaggtg
2341 aacttttgga ttggaactcg atttctgact gggttggaag gcaagagagc cccgaaagct
2401 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa
2461 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta
2521 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat
2581 ttaagtattg tttgtgcact tgccgatcta tgcggtgtga aataccgcac agatgcgtaa
2641 ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa
2701 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
2761 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
2821 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
2881 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
2941 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
3001 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
3061 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg
3121 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
3181 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
3241 gttttcccag tcacgacgtt gtaaaacgac ggccagtcgt ccaagctttc gcgagctcga
3301 gatcccgagc tttgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt
3361 tcagtataat gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata
3421 taaataacgt tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag
3481 gttgtctaac tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc
3541 gtgacataac taattacatg atatcgacaa aggaaaaggg gcctgtttac tcacaggctt
3601 ttttcaagta ggtaattaag tcgtttctgt ctttttcctt cttcaaccca ccaaaggcca
3661 tcttggtact tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
3721 tttttttttt tttttttttt tttttttttt tttttttttt tttcatagaa ataatacaga
3781 agtagatgtt gaattagatt aaactgaaga tatataattt attggaaaat acatagagct
3841 ttttgttgat gcgcttaagc gatcaattca acaacaccac cagcagctct gattttttct
3901 tcagccaact tggagacgaa tctagctttg acgataactg gaacatttgg aattctaccc
3961 ttacccaaga tcttaccgta accggctgcc aaagtgtcaa taactggagc agtttcctta
4021 gaagcagatt tcaagtattg gtctctcttg tcttctggga tcaatgtcca caatttgtcc
4081 aagttcaaga ctggcttcca gaaatgagct tgttgcttgt ggaagtatct cataccaacc
4141 ttaccgaaat aacctggatg gtatttatcc atgttaattc tgtggtgatg ttgaccaccg
4201 gccatacctc taccaccggg gtgctttctg tgcttaccga tacgaccttt accggctgag
4261 acgtgacctc tgtgctttct agtcttagtg aatctggaag gcattcttga ttagttggat
4321 gattgttctg ggatttaatg caaaaaaatc actaagaagg aaaaaaatca acggagaaag
4381 caaacgccat cttaaatata cgggatacag atgaaaggtt tgaacctatc tgggaaaata
4441 cgcattaaac aagcgaaaaa ctgcgaggaa aattgtttgc gtctctgcgg gctattcacg
4501 cgccagagga aaataggaaa aataacaggg cattagaaaa ataattttga ttttggtaat
4561 gtgtgggtcc ctggtgtaca gatgttacat tggttacagt actcttgttt ttgctgtgtt
4621 tttcgatgaa tctccaaaat ggttgttagc acatggaaga gtcaccgatg ctaagttatc
4681 tctatgtaag ctacgtggcg tgacttttga tgaagccgca caagagatac aggattggca
4741 actgcaaata gaatctgggg atctagatat ccttttgttg tttccgggtg tacaatatgg
4801 acttcctctt ttctggcaac caaacccata catcgggatt cctataatac cttcgttggt
4861 ctccctaaca tgtaggtggc ggaggggaga tatacaatag aacagatacc agacaagaca
4921 taatgggcta aacaagacta caccaattac actgcctcat tgatggtggt acataacgaa
4981 ctaatactgt agccctagac ttgatagcca tcatcatatc gaagtttcac tacccttttt
5041 ccatttgcca tctattgaag taataatagg cgcatgcaac ttcttttctt tttttttctt
5101 ttctctctcc cccgttgttg tctcaccata tccgcaatga caaaaaaaat gatggaagac
5161 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga
5221 tgaggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca cactactctc
5281 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaagttt
5341 gccgctttgc tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat
5401 tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag
5461 catacaatca actccaagct tgaagcaagc ctcctgaaag atgaagctac tgtcttctat
5521 cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa
5581 gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc
5641 tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct
5701 atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca
5761 ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt
5821 cacagataga ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat
5881 aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc
5941 gccggaattc atggcttacc catacgatgt tccagattac gctagcttgg gtggtcatat
6001 ggctgcgccg agggcggggc ggggtgcagg ctggagcctt cgggcatggc gggctttggg
6061 gggcattcgc tgggggagga gaccccgttt gacccctgac ctccgggccc tgctgacgtc
6121 aggaacttct gacccccggg cccgagtgac ttatgggacc cccagtctct gggcccggtt
6181 gtctgttggg gtcactgaac cccgagcatg cctgacgtct gggaccccgg gtccccgggc
6241 acaactgact gcggtgaccc cagataccag gacccgggag gcctcagaga actctggaac
6301 ccgttcgcgc gcgtggctgg cggtggcgct gggcgctggg ggggcagtgc tgttgttgtt
6361 gtggggcggg ggtcggggtc ctccggccgt cctcgccgcc gtccctagcc cgccgcccgc
6421 ttctccccgg agtcagtaca acttcatcgc agatgtggtg gagaagacag cacctgccgt
6481 ggtctatatc gagatcctgg accggcaccc tttcttgggc cgcgaggtcc ctatctcgaa
6541 cggctcagga ttcgtggtgg ctgccgatgg gctcattgtc accaacgccc atgtggtggc
6601 tgatcggcgc agagtccgtg tgagactgct aagcggcgac acgtatgagg ccgtggtcac
6661 agctgtggat cccgtggcag acatcgcaac gctgaggatt cagactaagg agcctctccc
6721 cacgctgcct ctgggacgct cagctgatgt ccggcaaggg gagtttgttg ttgccatggg
6781 aagtcccttt gcactgcaga acacgatcac atccggcatt gttagctctg ctcagcgtcc
6841 agccagagac ctgggactcc cccaaaccaa tgtggaatac attcaaactg atgcagctat
6901 tgattttgga aacgccggcg gtcccctggt taacctggat ggggaggtga ttggagtgaa
6961 caccatgaag gtcacagctg gaatctcctt tgccatccct tctgatcgtc ttcgagagtt
7021 tctgcatcgt ggggaaaaga agaattcctc ctccggaatc agtgggtccc agcggcgcta
7081 cattggggtg atgatgctga ccctgagtcc cagcatcctt gctgaactac agcttcgaga
7141 accaagcttt cccgatgttc agcatggtgt actcatccat aaagtcatcc tgggctcccc
7201 tgcacaccgg gctggtctgc ggcctggtga tgtgattttg gccattgggg agcagatggt
7261 acaaaatgct gaagatgttt atgaagctgt tcgaacccaa tcccagttgg cagtgcagat
7321 ccggcgggga cgagaaacac tgaccttata tgtgacccct gaggtcacag aatgaggcca
7381 tggaggcccc ggggatccgt cgacctgcag ccaagctaat tccgggcgaa tttcttatga
7441 tttatgattt ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt
7501 gactcttagg ttttaaaacg aaaattcttg ttcttgagta actctttcct gtaggtcagg
7561 ttgctttctc aggtatagca tgaggtcgct cttattgacc acacctctac cggcatgcaa
7621 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
7681 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgaggt
7741 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
7801 agctggatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
7861 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
7921 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
7981 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
8041 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
8101 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
8161 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
8221 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
8281 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
8341 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
8401 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
8461 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
8521 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
8581 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
8641 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
8701 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
8761 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
8821 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
8881 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
8941 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
9001 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
9061 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
9121 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
9181 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
9241 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
9301 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
9361 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
9421 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
9481 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
9541 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
9601 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
9661 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
9721 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
9781 tgccacct
//