LOCUS LT727105 8445 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pEGFPC2-haEctn(69-3454), complete
sequence.
ACCESSION LT727105
VERSION LT727105.1
KEYWORDS .
SOURCE Mammalian expression vector pEGFPC2-haEctn(69-3454)
ORGANISM Mammalian expression vector pEGFPC2-haEctn(69-3454)
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 8445)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..8445
/organism="Mammalian expression vector
pEGFPC2-haEctn(69-3454)"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4552."
/db_xref="taxon:1945015"
regulatory complement(152..392)
/note="polyA"
/regulatory_class="terminator"
CDS complement(583..1377)
/transl_table=11
/note="neomycin resistance"
/protein_id="SJL87441.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
rep_origin complement(1437..1512)
/note="SV40 ori"
regulatory complement(1469..1750)
/note="SV40 early promoter"
/regulatory_class="promoter"
regulatory complement(1806..1901)
/note="amp"
/regulatory_class="promoter"
rep_origin 1902..2357
/note="f1 ori"
regulatory complement(2411..2507)
/note="polyA"
/regulatory_class="terminator"
regulatory 2411..2507
/note="polyA"
/regulatory_class="terminator"
misc_feature complement(2658..2983)
/note="3FR"
misc_feature complement(2984..3604)
/note="3UTR"
CDS complement(3605..6325)
/transl_table=11
/note="haEctn"
/protein_id="SJL87442.1"
/translation="MTAVHAGNINFKWDPKSLEIRTLAVERLLEPLVTQVTTLVNTNS
KGPSNKKRGRSKKAHVLAASVEQATENFLEKGDKIAKESQFLKEELVAAVEDVRKQGD
LMKAAAGEFADDPCSSVKRGNMVRAARALLSAVTRLLILADMADVYKLLVQLKVVEDG
ILKLRNAGNEQDLGIQYKALKPEVDKLNIMAAKRQQELKDVGHRDQMAAARGILQKNV
PILYTASQACLQHPDVAAYKANRDLIYKQLQQAVTGISNAAQATASDDASQHQGGGGG
ELAYALNNFDKQIIVDPLSFSEERFRPSLEERLESIISGAALMADSSCTRDDRRERIV
AECNAVRKALQDLLSEYMGNAGRKERSDALNSAIDKMTKKTRDLRRQLRKAVMDHVSD
SFLETNVPLLVLIEAAKNGNEKEVKEYAQVFREHANKLIEVANLACSISNNEEGVKLV
RMSASQLEALCPQVINAALALAAKPQSKLAQENMDLFKEQWEKQVRVLTDAVDDITSI
DDFLAVSENHILEDVNKCVIALQEKDVDGLDRTAGAIRGRAARVIHVVTSEMDNYEPG
VYTEKVLEATKLLSNTVMPRFTEQVEAAVEALSSDPAQPMDENEFIDASRLVYDGIRD
IRKAVLMIRTPEELDDSDFETEDFDVRSRTSVQTEDDQLIAGQSARAIMAQLPQEQKA
KIAEQVASFQEEKSKLDAEVSKWDDSGNDIIVLAKQMCMIMMEMTDFTRGKGPLKNTS
DVISAAKKIAEAGSRMDKLGRTIADHCPDSACKQDLLAYLQRIALYCHHLNICSKVKA
EVQNLGGELVVSGVDSAMSLIQAAKNLMNAVVQTVKASYVASTKYQKSQGMASLNLPA
VSWKMKAPEKKPLVKREKQDETQTKIKRASQKKHVNPVQALSEFKAMDSI"
CDS complement(<3689..7105)
/transl_table=11
/note="EGFP"
/protein_id="SJL87443.1"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
MVLLEFVTAAGITLGMDELYKSGRTQISSSSFEFPLAQLAAEMTAVHAGNINFKWDPK
SLEIRTLAVERLLEPLVTQVTTLVNTNSKGPSNKKRGRSKKAHVLAASVEQATENFLE
KGDKIAKESQFLKEELVAAVEDVRKQGDLMKAAAGEFADDPCSSVKRGNMVRAARALL
SAVTRLLILADMADVYKLLVQLKVVEDGILKLRNAGNEQDLGIQYKALKPEVDKLNIM
AAKRQQELKDVGHRDQMAAARGILQKNVPILYTASQACLQHPDVAAYKANRDLIYKQL
QQAVTGISNAAQATASDDASQHQGGGGGELAYALNNFDKQIIVDPLSFSEERFRPSLE
ERLESIISGAALMADSSCTRDDRRERIVAECNAVRKALQDLLSEYMGNAGRKERSDAL
NSAIDKMTKKTRDLRRQLRKAVMDHVSDSFLETNVPLLVLIEAAKNGNEKEVKEYAQV
FREHANKLIEVANLACSISNNEEGVKLVRMSASQLEALCPQVINAALALAAKPQSKLA
QENMDLFKEQWEKQVRVLTDAVDDITSIDDFLAVSENHILEDVNKCVIALQEKDVDGL
DRTAGAIRGRAARVIHVVTSEMDNYEPGVYTEKVLEATKLLSNTVMPRFTEQVEAAVE
ALSSDPAQPMDENEFIDASRLVYDGIRDIRKAVLMIRTPEELDDSDFETEDFDVRSRT
SVQTEDDQLIAGQSARAIMAQLPQEQKAKIAEQVASFQEEKSKLDAEVSKWDDSGNDI
IVLAKQMCMIMMEMTDFTRGKGPLKNTSDVISAAKKIAEAGSRMDKLGRTIADHCPDS
ACKQDLLAYLQRIALYCHHLNICSKVKAEVQNLGGELVVSGVDSAMSLIQAAKNLMNA
VVQTVKASYVASTKYQKSQGMASLNLPAVSWKMKAPEKKPLVKREKQDETQ"
regulatory complement(7150..7717)
/note="hCMV"
/regulatory_class="promoter"
rep_origin complement(7724..8050)
/note="pMB1 ori"
BASE COUNT 1946 a 2167 c 2112 g 2220 t
ORIGIN
1 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
61 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
121 tatatgagta acctgaggct atggcagggc ctgccgcccc gacgttggct gcgagccctg
181 ggccttcacc cgaacttggg gggtggggtg gggaaaagga agaaacgcgg gcgtattggc
241 cccaatgggg tctcggtggg gtatcgacag agtgccagcc ctgggaccga accccgcgtt
301 tatgaacaaa cgacccaaca ccgtgcgttt tattctgtct ttttattgcc gtcatagcgc
361 gggttccttc cggtattgtc tccttccgtg tttcagttag cctcccccta gggtgggcga
421 agaactccag catgagatcc ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga
481 ttccgaagcc caacctttca tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt
541 tgggcgtcgc ttggtcggtc atttcgaacc ccagagtccc gctcagaaga actcgtcaag
601 aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa
661 gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc
721 ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt
781 ttccaccatg atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc
841 gggcatgctc gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc
901 gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg
961 atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat
1021 tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg
1081 ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac
1141 agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcttgcag
1201 ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga
1261 cagccggaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa
1321 tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg
1381 aaacgatcct catcctgtct cttgatcgat ctttgcaaaa gcctaggcct ccaaaaaagc
1441 ctcctcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa
1501 aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga
1561 tgggcggagt taggggcggg actatggttg ctgactaatt gagatgcatg ctttgcatac
1621 ttctgcctgc tggggagcct ggggactttc cacacctggt tgctgactaa ttgagatgca
1681 tgctttgcat acttctgcct gctggggagc ctggggactt tccacaccct aactgacaca
1741 cattccacag ctggttcttt ccgcctcagg actcttcctt tttcaatatt attgaagcat
1801 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
1861 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc
1921 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
1981 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
2041 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
2101 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
2161 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
2221 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
2281 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
2341 attaacgctt acaatttacg cgttaagata cattgatgag tttggacaaa ccacaactag
2401 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
2461 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
2521 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc
2581 tgattatgat cagttatcta gatccggtgg atcccgggcc cgcggtaccg tcgactctag
2641 attttttttt tttttttgtc gtggtttcat agtggacttt attgggaaaa gttagttagt
2701 gtaaaaaaca ttcaaattaa ttcataaaat ttaaataagg tgatagtaaa ttataccttg
2761 tagttaatag taatcaatca atcaatcact acagtaatca caaataaggt aaagtctaaa
2821 ttactgcctt agcaaacact atgttgtcag gtttttctgc tgcaagccca aggcgggaaa
2881 cactgcagtt attagaagtg agcccaatga tgaatttgca tttgaagctg ggagaaagag
2941 gaaaaaaagt gtgttctgat tatggcatcg agacactgta gccgcctaaa aaagcaactt
3001 tattaatgtc ctgcagcagc ggtacattag taattataac aatgcattaa aattttcatt
3061 tcatgtcata gagaatcagt tttcttcatg atacattatg ttttactgag tgagtttgtc
3121 cctccagaga cctttctggg gaacatgcct tctccaggga ctgcttccta agatgcccag
3181 gttgcttacc acaggtcatc tttggtcatt taaaagcagt caagctgtct ggactgcacc
3241 ctctcaactg aagttgagcc aaattccctt ctctcaaacc ctaacgccgc catcactaag
3301 agaatgggca tctcggcttg gtcagagctt actaacaagc taatgtggga ttctctttgg
3361 ttatgaattt tatttttatt taagcataca aaaaatacag ctagaagtag gctcctgcat
3421 tctaaaagca tttttaatca acttaaacta tgttcttgga aaattcacca cctggactgg
3481 ttcagtctgc ccatttccct gtggaatcta gtatcagtgt tgtatttagc aaattcattt
3541 gagtgacgaa cagtgactga tattcaggag ccccgagggg tgggggcggc cggcctgggc
3601 agacttagat gctgtccata gctttgaact cgctgagggc ctgcaccggg ttcacgtgct
3661 tcttctgaga tgcccgttta atcttggtct gtgtctcatc ctgtttctct ctcttcacca
3721 atggcttttt ctctggtgcc ttcatcttcc atgacacagc aggaaggttg agggaagcca
3781 taccctgtga cttttggtat ttggtagagg cgacgtagga tgccttcact gtctgcacca
3841 cagcattcat caagttcttg gctgcctgga tcagggacat ggcgctgtcc accccagaga
3901 caacaagctc cccgccgaga ttctgcacct cggccttgac cttgctgcag atgttcagat
3961 ggtggcagta gagggcgatg cgttgcaggt aggccagcag gtcctgcttg caagccgagt
4021 cggggcaatg gtctgcaatg gtgcggccaa gcttgtccat cctggatcct gcctcagcaa
4081 ttttcttggc agcactgatg acatccgatg tatttttgag tggtccttta cctcgggtaa
4141 agtctgtcat ctccatcata atcatgcaca tctgcttggc cagcacaatg atgtcattgc
4201 cactgtcgtc ccatttggac acttcagcat ccagcttgct cttttcttcc tggaagctgg
4261 ccacctgttc cgcaatcttc gctttttgct cctggggaag ctgagccatg atcgcccggg
4321 cactctggcc agctatcagc tgatcgtctt ctgtctggac gctcgtcctg cttctgacat
4381 caaaatcttc tgtctcaaag tcagagtcat ccaactcctc aggggtcctt atcatcagca
4441 ctgctttcct gatgtcccgg atgccatcat ataccaggcg ggaagcatcg ataaactcat
4501 tctcatccat gggctgggca gggtccgagc tgagggcttc cacggctgct tctacttgct
4561 cagtaaaacg tggcatgact gtgttggaga gcagcttagt ggcttccaga accttctctg
4621 tgtagactcc tggctcatag ttgtccatct ctgaggtgac tacgtgaatg acccgggctg
4681 cccggcctcg aattgcacca gctgtgcggt ccaggccatc cacatccttc tcttggagag
4741 caatgacaca tttgttcaca tcttccaaaa tgtgattctc tgagacagcc aagaagtcat
4801 caatggaagt aatgtcatcg acagcatctg tgagaacacg gacttgtttt tcccattgtt
4861 ctttaaaaag atccatgttc tcttgggcca gtttactctg tggttttgct gctaaagcca
4921 gtgcagcatt aataacctga ggacagaggg cttctaactg gcttgcagac attcgaacaa
4981 gctttacacc ttcttcatta tttgagatgg aacaggccaa gttggcaacc tcaatcaatt
5041 tgttggcatg ttcacggaaa acttgggcat actccttaac ttctttctca tttccattct
5101 ttgcagcttc aatcaatacc aaaagtggaa cattggtttc caggaaagaa tctgaaacgt
5161 ggtccatgac agctttgcgg agctgtctac gcaagtccct ggtcttcttg gtcattttat
5221 ctattgcaga attgagtgca tcacttcttt ctttacgtcc agcattgccc atgtactccg
5281 aaagcaggtc ctgcagggcc ttgcggacag cattacactc tgccacaatt cgctcacgac
5341 ggtcatcacg cgtgcaggag gagtcggcca tcaaggcagc cccactaatg atgctttcca
5401 gacgctcctc cagggaaggc ctaaagcgct cctcgctgaa gctcaagggg tccacaatga
5461 tttgtttgtc aaagttattg agtgcatatg ccagttctcc tcctcctcca ccctggtgct
5521 gtgaggcatc gtctgaggca gtggcctggg ctgcattgga aatgcctgtg accgcctgct
5581 gcagctgctt gtatatcagg tcccggttgg ccttataggc tgcgacatca gggtgctgta
5641 ggcatgcctg ggatgcagta tagaggatcg gaacgttctt ctgcaggatt cctctagctg
5701 cagccatctg atcacgatgg ccaacatctt tcaattcctg ttgtcttttg gctgccataa
5761 tgttcagctt atccacttca ggttttaggg ctttatactg gattcctaag tcttgttcat
5821 tgccagcatt cctcaacttc aagataccat cttccacaac tttcagctga acaagtaatt
5881 tgtagacatc tgccatgtca gccaaaatca gcaaccgggt aacagcagag agcaaagctc
5941 gagctgcccg aaccatgttg cctcgcttca cagaagagca gggatcatct gcgaactctc
6001 ctgcagcagc cttcatcaaa tcaccttgtt ttcgaacatc ttctacagca gccacaagct
6061 cctccttgag aaactggctc tccttcgcaa ttttatcccc cttctccaag aaattctcag
6121 ttgcttgttc aacagatgca gccaaaacat gggccttctt agaacgacct ctcttcttat
6181 tagagggccc tttactattg gtgtttacaa gggttgtaac ctgtgtaaca agaggctcca
6241 acagtctctc aactgccaga gtcctgatct ctagactttt aggatcccac ttgaagttta
6301 tgttgcctgc atggacagca gtcatttctg cggctagctg ggcgagcggg aattcgaagc
6361 ttgagctcga gatctgagtc cggccggact tgtacagctc gtccatgccg agagtgatcc
6421 cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc
6481 tcagggcgga ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga
6541 tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc
6601 ggatcttgaa gttcaccttg atgccgttct tctgcttgtc ggccatgata tagacgttgt
6661 ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga
6721 tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg
6781 tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca
6841 tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg aagcactgca
6901 cgccgtaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc
6961 agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc
7021 tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga
7081 acagctcctc gcccttgctc accatggtgg cgaccggtag cgctagcgga tctgacggtt
7141 cactaaacca gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt
7201 caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa
7261 actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc
7321 acgcccattg atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta
7381 gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg
7441 ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac
7501 tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat
7561 tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg
7621 tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg
7681 aactaatgac cccgtaattg attactatta ataactaatg catggcggta atacggttat
7741 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
7801 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
7861 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
7921 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
7981 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
8041 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
8101 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
8161 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
8221 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
8281 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
8341 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
8401 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacgg
//