LOCUS LT727095 5338 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-E-hABIN-1-MAD, complete
sequence.
ACCESSION LT727095
VERSION LT727095.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-E-hABIN-1-MAD
ORGANISM Mammalian expression vector pCAGGS-E-hABIN-1-MAD
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5338)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5338
/organism="Mammalian expression vector
pCAGGS-E-hABIN-1-MAD"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5131."
/db_xref="taxon:1944770"
misc_feature 11..49
/note="E-tag"
CDS <59..>532
/codon_start=1
/transl_table=11
/note="hABIN-1-MAD"
/protein_id="SJL87415.1"
/translation="TPPSSPPTAFGSPEGAGALLRKQELVTQNELLKQQVKIFEEDFQ
RERSDRERMNEEKEELKKQVEKLQAQVTLSNAQLKAFKDEEKAREALRQQKRKAKASG
ERYHVEPHPEHLCGAYPYAYPPMPAMVPHHGFEDWSQIRYPPPPMAMEHPPPLPNS"
regulatory 623..714
/note="3' UTR of rabbit beta-globin, incl. polyA"
/regulatory_class="terminator"
misc_feature 715..1071
/note="3' flanking region of rabbit beta-globin"
regulatory complement(1149..1231)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1373..1448
/note="SV40 ori"
regulatory 1479..1553
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(1479..1553)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(1668..2069)
/note="pMB1 ori"
CDS complement(2610..3470)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87416.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3606..3983
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 3989..5330
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4358..5282
/note="intron AG promoter"
BASE COUNT 1144 a 1441 c 1491 g 1262 t
ORIGIN
1 ttccaccatg ggtgcgccgg tgccgtatcc ggacccgctg gaaccgcgtg cggccgccac
61 cccgccatca tctccaccaa cagcatttgg gagcccagaa ggagcagggg ccctcctaag
121 gaaacaggag ctggtcacgc agaatgagtt gctgaaacag caggtgaaga tcttcgagga
181 ggacttccag agggagcgca gtgatcgtga gcgcatgaat gaggagaagg aagagctgaa
241 gaagcaagtg gagaagctgc aggcccaggt caccctgtca aatgcccagc taaaagcatt
301 caaagatgag gagaaggcaa gagaagccct cagacagcag aagaggaaag caaaggcctc
361 aggagagcgt taccatgtgg agccccaccc agaacatctc tgcggggcct acccctacgc
421 ctacccgccc atgccagcca tggtgccaca ccatggcttc gaggactggt cccagatccg
481 ctacccccct ccccccatgg ccatggagca cccgccccca ctccccaact cgtgactcga
541 ggaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc
601 cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat
661 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt
721 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa
781 acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat
841 gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc
901 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt
961 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct
1021 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct
1081 gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
1141 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg gtgcctaatg
1201 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
1261 gtcgtgccag cggatccgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
1321 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
1381 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
1441 ggaggctttt ttggaggcct aggcttttgc aaaaagctaa cttgtttatt gcagcttata
1501 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
1561 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgctgca
1621 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
1681 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
1741 aaaggcggta atacggttat ccacagaatc agggataacg caggaaagaa catgtgagca
1801 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
1861 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
1921 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
1981 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
2041 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
2101 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
2161 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
2221 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
2281 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
2341 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
2401 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
2461 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
2521 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
2581 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
2641 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
2701 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
2761 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
2821 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
2881 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
2941 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
3001 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
3061 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
3121 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
3181 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
3241 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
3301 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
3361 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
3421 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
3481 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
3541 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
3601 gggtcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
3661 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
3721 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
3781 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
3841 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
3901 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
3961 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
4021 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
4081 agcgatgggg gcgggggggg ggggggggcc ccccccaggc ggggcggggc ggggcgaggg
4141 gcggggcggg gcgaggcgga aaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
4201 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
4261 ggcgggagtc gttgcgcgct gccttccccc cgtgccccgc tccgccgccg cctcgcgccg
4321 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
4381 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
4441 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc
4501 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga
4561 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc
4621 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg
4681 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct
4741 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg
4801 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg
4861 ggcggggccg cctcgggccg gggagggctc gggggaaggg gcgcggcggc ccccggagcg
4921 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
4981 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
5041 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
5101 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
5161 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
5221 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
5281 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgaa
//