LOCUS LT727093 2711 bp DNA circular SYN 06-FEB-2017
DEFINITION Bacterial expression vector pPLc281, complete sequence.
ACCESSION LT727093
VERSION LT727093.1
KEYWORDS .
SOURCE Bacterial expression vector pPLc281
ORGANISM Bacterial expression vector pPLc281
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 2711)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..2711
/organism="Bacterial expression vector pPLc281"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 512."
/db_xref="taxon:1944694"
rep_origin complement(296..698)
/note="pMB1 ori"
CDS complement(1239..2099)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87412.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 2465..2595
/note="PL"
/regulatory_class="promoter"
BASE COUNT 698 a 686 c 671 g 656 t
ORIGIN
1 ttccaagctt ggctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
61 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
121 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga
181 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac
241 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct
301 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
361 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
421 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
481 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
541 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
601 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
661 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
721 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
781 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
841 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
901 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
961 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
1021 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
1081 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
1141 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
1201 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
1261 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
1321 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
1381 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
1441 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
1501 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc
1561 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
1621 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
1681 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
1741 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
1801 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg
1861 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
1921 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
1981 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
2041 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
2101 ctcttccttt ttcaatatta tgtaagcaga cagttttatt gttcatgatg atatattttt
2161 atcttgtgca atgtaacatc agagattttg agacacaacg tggctttgtt gaataaatcg
2221 aacttttgct gagttgaagg atcagatcac gcatcttccc gacaacgcag accgttccgt
2281 ggcaaagcaa aagttcaaaa tcaccaactg gtccacctac aacaaagctc tcatcaaccg
2341 tggctccctc actttctggc tggatgatgg ggcgattcag gcctggtatg agtcagcaac
2401 accttcttca cgaggcagac ctcagcgccg gtgatgccgg ccacgatgcg tccggcgtag
2461 aggatctctc acctaccaaa caatgccccc ctgcaaaaaa taaattcata taaaaaacat
2521 acagataacc atctgcggtg ataaattatc tctggcggtg ttgacataaa taccactggc
2581 ggtgatactg agcacatcag caggacgcac tgaccaccat gaaggtgacg ctcttaaaat
2641 taagccctga agaagggcag cattcaaagc agaaggcttt ggggtgtgtg atacgaaacg
2701 aagcattgga a
//