LOCUS LT727091 7589 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pEF6-hXIAP, complete sequence.
ACCESSION LT727091
VERSION LT727091.1
KEYWORDS .
SOURCE Mammalian expression vector pEF6-hXIAP
ORGANISM Mammalian expression vector pEF6-hXIAP
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7589)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..7589
/organism="Mammalian expression vector pEF6-hXIAP"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5110."
/db_xref="taxon:1945007"
regulatory 386..1529
/note="hEF1alpha promoter"
/regulatory_class="promoter"
misc_feature 582..1520
/note="intron hEF1alpha promoter"
regulatory 1546..1562
/note="T7 promoter"
/regulatory_class="promoter"
CDS 1934..3427
/transl_table=11
/note="hXIAP"
/protein_id="SJL87406.1"
/translation="MTFNSFEGSKTCVPADINKEEEFVEEFNRLKTFANFPSGSPVSA
STLARAGFLYTGEGDTVRCFSCHAAVDRWQYGDSAVGRHRKVSPNCRFINGFYLENSA
TQSTNSGIQNGQYKVENYLGSRDHFALDRPSETHADYLLRTGQVVDISDTIYPRNPAM
YCEEARLKSFQNWPDYAHLTPRELASAGLYYTGIGDQVQCFCCGGKLKNWEPCDRAWS
EHRRHFPNCFFVLGRNLNIRSESDAVSSDRNFPNSTNLPRNPSMADYEARIFTFGTWI
YSVNKEQLARAGFYALGEGDKVKCFHCGGGLTDWKPSEDPWEQHAKWYPGCKYLLEQK
GQEYINNIHLTHSLEECLVRTTEKTPSLTRRIDDTIFQNPMVQEAIRMGFSFKDIKKI
MEEKIQISGSNYKSLEVLVADLVNAQKDSMQDESSQTSLQKEISTEEQLRRLQEEKLC
KICMDRNIAIVFVPCGHLVTCKQCAEAVDKCPMCYTVITFKQKIFMS"
misc_feature 3471..3500
/note="Myc epitope"
misc_feature 3516..3533
/note="His-tag"
regulatory 3562..3786
/note="bovine growth hormone polyA"
/regulatory_class="terminator"
rep_origin 3832..4260
/note="f1 ori"
regulatory 4265..4546
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 4503..4578
/note="SV40 ori"
regulatory 4651..4716
/note="EM7 promoter"
/regulatory_class="promoter"
CDS 4717..5115
/transl_table=11
/note="blasticidin"
/protein_id="SJL87407.1"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFT
GVNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHP
GIKAIVKDSDGQPTAVGIRELLPSGYVWEG"
regulatory complement(5273..5347)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 5273..5347
/note="SV40 polyA early"
/regulatory_class="terminator"
rep_origin complement(5661..6052)
/note="pMB1 ori"
CDS complement(6593..7453)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87408.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1880 a 1787 c 1961 g 1961 t
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga
301 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg
361 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
421 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
481 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
541 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt
601 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct
661 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga
721 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct
781 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt
841 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc
901 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg
961 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac
1021 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc
1081 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag
1141 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct
1201 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg
1261 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga
1321 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
1381 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg
1441 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa
1501 gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta
1561 tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac tagtccagtg
1621 tggtggaatt ctgcagatgc ttcggtatcg ataagctatg gagcaaaagc tcatttctga
1681 agaggacttg aatgaaatgg agcaaaagct catttctgaa gaggacttga atgaaatgga
1741 gcaaaagctc atttctgaag aggacttgaa tgaaatggag caaaagctca tttctgaaga
1801 ggacttgaat gaaatggagc aaaagctcat ttctgaagag gacttgaatg aaatggagag
1861 cttgggcgac ctcaccatgg agcaaaagct catttctgaa gaggacttga attcctgcag
1921 cccgggggga tccatgactt ttaacagttt tgaaggatct aaaacttgtg tacctgcaga
1981 catcaataag gaagaagaat ttgtagaaga gtttaataga ttaaaaactt ttgctaattt
2041 tccaagtggt agtcctgttt cagcatcaac actggcacga gcagggtttc tttatactgg
2101 tgaaggagat accgtgcggt gctttagttg tcatgcagct gtagatagat ggcaatatgg
2161 agactcagca gttggaagac acaggaaagt atccccaaat tgcagattta tcaacggctt
2221 ttatcttgaa aatagtgcca cgcagtctac aaattctggt atccagaatg gtcagtacaa
2281 agttgaaaac tatctgggaa gcagagatca ttttgcctta gacaggccat ctgagacaca
2341 tgcagactat cttttgagaa ctgggcaggt tgtagatata tcagacacca tatacccgag
2401 gaaccctgcc atgtattgtg aagaagctag attaaagtcc tttcagaact ggccagacta
2461 tgctcaccta accccaagag agttagcaag tgctggactc tactacacag gtattggtga
2521 ccaagtgcag tgcttttgtt gtggtggaaa actgaaaaat tgggaacctt gtgatcgtgc
2581 ctggtcagaa cacaggcgac actttcctaa ttgcttcttt gttttgggcc ggaatcttaa
2641 tattcgaagt gaatctgatg ctgtgagttc tgataggaat ttcccaaatt caacaaatct
2701 tccaagaaat ccatccatgg cagattatga agcacggatc tttacttttg ggacatggat
2761 atactcagtt aacaaggagc agcttgcaag agctggattt tatgctttag gtgaaggtga
2821 taaagtaaag tgctttcact gtggaggagg gctaactgat tggaagccca gtgaagaccc
2881 ttgggaacaa catgctaaat ggtatccagg gtgcaaatat ctgttagaac agaagggaca
2941 agaatatata aacaatattc atttaactca ttcacttgag gagtgtctgg taagaactac
3001 tgagaaaaca ccatcactaa ctagaagaat tgatgatacc atcttccaaa atcctatggt
3061 acaagaagct atacgaatgg ggttcagttt caaggacatt aagaaaataa tggaggaaaa
3121 aattcagata tctgggagca actataaatc acttgaggtt ctggttgcag atctagtgaa
3181 tgctcagaaa gacagtatgc aagatgagtc aagtcagact tcattacaga aagagattag
3241 tactgaagag cagctaaggc gcctgcaaga ggagaagctt tgcaaaatct gtatggatag
3301 aaatattgct atcgtttttg ttccttgtgg acatctagtc acttgtaaac aatgtgctga
3361 agcagttgac aagtgtccca tgtgctacac agtcattact ttcaagcaaa aaatttttat
3421 gtcttaatct aactctatag tagctcgagc atgcatctag agggcccttc gaacaaaaac
3481 tcatctcaga agaggatctg aatatgcata ccggtcatca tcaccatcac cattgagttt
3541 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
3601 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
3661 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
3721 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
3781 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
3841 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
3901 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
3961 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
4021 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
4081 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
4141 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
4201 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
4261 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
4321 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
4381 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
4441 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
4501 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
4561 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
4621 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc
4681 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagccttt gtctcaagaa
4741 gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac
4801 tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta
4861 tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg
4921 gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc
4981 ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg
5041 aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat
5101 gtgtgggagg gctaagcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt
5161 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
5221 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
5281 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
5341 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
5401 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
5461 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
5521 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
5581 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
5641 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
5701 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
5761 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc caggaaccgt aaaaaggccg
5821 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
5881 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
5941 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
6001 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
6061 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
6121 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
6181 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
6241 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
6301 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
6361 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
6421 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
6481 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
6541 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
6601 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
6661 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
6721 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
6781 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
6841 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
6901 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
6961 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
7021 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
7081 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
7141 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
7201 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
7261 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
7321 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
7381 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
7441 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
7501 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
7561 catttccccg aaaagtgcca cctgacgtc
//