LOCUS LT727090 6581 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pEF6hBCL2, complete sequence.
ACCESSION LT727090
VERSION LT727090.1
KEYWORDS .
SOURCE Mammalian expression vector pEF6hBCL2
ORGANISM Mammalian expression vector pEF6hBCL2
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6581)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6581
/organism="Mammalian expression vector pEF6hBCL2"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5109."
/db_xref="taxon:1944999"
regulatory 386..1529
/note="hEF1alpha promoter"
/regulatory_class="promoter"
misc_feature 582..1520
/note="intron hEF1alpha promoter"
regulatory 1546..1562
/note="T7 promoter"
/regulatory_class="promoter"
CDS 1664..2383
/transl_table=11
/note="human BCL2"
/protein_id="SJL87403.1"
/translation="MAHAGRTGYDNREIVMKYIHYKLSQRGYEWDAGDVGAAPPGAAP
APGIFSSQPGHTPHPAASRDPVARTSPLQTPAAPGAAAGPALSPVPPVVHLTLRQAGD
DFSRRYRRDFAEMSSQLHLTPFTARGRFATVVEELFRDGVNWGRIVAFFEFGGVMCVE
SVNREMSPLVDNIALWMTEYLNRHLHTWIQDNGGWDAFVELYGPSMRPLFDFSWLSLK
TLLSLALVGACITLGAYLGHK"
misc_feature 2463..2492
/note="Myc epitope"
misc_feature 2508..2525
/note="His-tag"
regulatory 2554..2778
/note="BGH polyA"
/regulatory_class="terminator"
rep_origin 2824..3252
/note="f1 ori"
regulatory 3257..3538
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 3495..3570
/note="SV40 ori"
regulatory 3643..3708
/note="EM7 promoter"
/regulatory_class="promoter"
CDS 3709..4107
/transl_table=11
/note="blasticidin resistance"
/protein_id="SJL87404.1"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFT
GVNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHP
GIKAIVKDSDGQPTAVGIRELLPSGYVWEG"
regulatory 4265..4339
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(4265..4339)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 4478..4561
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(4653..5044)
/note="pMB1 ori"
CDS complement(5585..6445)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87405.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1422 a 1736 c 1800 g 1623 t
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga
301 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg
361 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
421 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
481 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
541 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt
601 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct
661 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga
721 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct
781 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt
841 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc
901 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg
961 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac
1021 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc
1081 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag
1141 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct
1201 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg
1261 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga
1321 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
1381 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg
1441 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa
1501 gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta
1561 tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac tagtccagtg
1621 tggtggaatt cggcttgccc ccgttgcttt tcctctggga aggatggcgc acgctgggag
1681 aacggggtac gataaccggg agatagtgat gaagtacatc cattataagc tgtcgcagag
1741 gggctacgag tgggatgcgg gagatgtggg cgccgcgccc ccgggggccg cccccgcacc
1801 gggcatcttc tcctcccagc ccgggcacac gccccatcca gccgcatccc gcgacccggt
1861 cgccaggacc tcgccgctgc agaccccggc tgcccccggc gccgccgcgg ggcctgcgct
1921 cagcccggtg ccacctgtgg tccacctgac cctccgccag gccggcgacg acttctcccg
1981 ccgctaccgc cgcgacttcg ccgagatgtc cagccagctg cacctgacgc ccttcaccgc
2041 gcggggacgc tttgccacgg tggtggagga gctcttcagg gacggggtga actgggggag
2101 gattgtggcc ttctttgagt tcggtggggt catgtgtgtg gagagcgtca accgggagat
2161 gtcgcccctg gtggacaaca tcgccctgtg gatgactgag tacctgaacc ggcacctgca
2221 cacctggatc caggataacg gaggctggga tgcctttgtg gaactgtacg gccccagcat
2281 gcggcctctg tttgatttct cctggctgtc tctgaagact ctgctcagtt tggccctggt
2341 gggagcttgc atcaccctgg gtgcctatct gggccacaag tgaagtcaac atgcctgccc
2401 caaaagccga attctgcaga tatccagcac agtggcggcc gctcgagtct agagggccct
2461 tcgaacaaaa actcatctca gaagaggatc tgaatatgca taccggtcat catcaccatc
2521 accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
2581 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
2641 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
2701 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
2761 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
2821 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
2881 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
2941 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
3001 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
3061 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
3121 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
3181 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
3241 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
3301 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
3361 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
3421 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
3481 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
3541 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
3601 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg
3661 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagcct
3721 ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc
3781 atctctgaag actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact
3841 ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact
3901 gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg
3961 ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc
4021 aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg
4081 ccctctggtt atgtgtggga gggctaagca cttcgtggcc gaggagcagg actgacacgt
4141 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
4201 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
4261 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
4321 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
4381 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
4441 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
4501 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
4561 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
4621 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
4681 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
4741 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccaggaacc
4801 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
4861 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
4921 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
4981 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
5041 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
5101 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
5161 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
5221 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
5281 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
5341 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
5401 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
5461 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
5521 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
5581 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
5641 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
5701 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
5761 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
5821 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
5881 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
5941 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
6001 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
6061 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
6121 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
6181 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
6241 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
6301 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
6361 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
6421 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
6481 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
6541 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
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