LOCUS LT727088 7270 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pEF6crmA, complete sequence.
ACCESSION LT727088
VERSION LT727088.1
KEYWORDS .
SOURCE Mammalian expression vector pEF6crmA
ORGANISM Mammalian expression vector pEF6crmA
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7270)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..7270
/organism="Mammalian expression vector pEF6crmA"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5107."
/db_xref="taxon:1944989"
regulatory 386..1529
/note="hEF1a promoter"
/regulatory_class="promoter"
misc_feature 582..1520
/note="intron hEF1a promoter"
regulatory 1546..1562
/note="T7 promoter"
/regulatory_class="promoter"
misc_feature 1632..1924
/note="5' UTR of crmA"
CDS 1925..2950
/transl_table=11
/note="crmA"
/protein_id="SJL87397.1"
/translation="MDIFREIASSMKGENVFISPPSISSVLTILYYGANGSTAEQLSK
YVEKEADKNKDDISFKSMNKVYGRYSAVFKDSFLRKIGDNFQTVDFTDCRTVDAINKC
VDIFTEGKINPLLDEPLSPDTCLLAISAVYFKAKWLMPFEKEFTSDYPFYVSPTEMVD
VSMMSMYGEAFNHASVKESFGNFSIIELPYVGDTSMVVILPDNIDGLESIEQNLTDTN
FKKWCDSMDAMFIDVHIPKFKVTGSYNLVDALVKLGLTEVFGSTGDYSNMCNSDVSVD
AMIHKTYIDVNEEYTEAAAATCALVADCASTVTNEFCADHPFIYVIRHVDGKILFVGR
YCSPTTN"
misc_feature 2951..3096
/note="3' UTR of crmA"
misc_feature 3152..3181
/note="Myc epitope"
misc_feature 3197..3214
/note="His-tag"
regulatory 3243..3467
/note="BGH polyA"
/regulatory_class="terminator"
rep_origin 3513..3941
/note="f1 ori"
regulatory 3946..4227
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 4184..4259
/note="SV40 ori"
regulatory 4332..4397
/note="synthetic EM7 promoter"
/regulatory_class="promoter"
CDS 4398..4796
/transl_table=11
/note="blasticidin resistance"
/protein_id="SJL87398.1"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFT
GVNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHP
GIKAIVKDSDGQPTAVGIRELLPSGYVWEG"
regulatory complement(4954..5028)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 4954..5028
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(5167..5250)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(5342..5733)
/note="pMB1 ori"
CDS complement(6274..7134)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87399.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1792 a 1708 c 1836 g 1934 t
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga
301 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg
361 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
421 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
481 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
541 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt
601 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct
661 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga
721 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct
781 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt
841 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc
901 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg
961 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac
1021 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc
1081 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag
1141 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct
1201 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg
1261 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga
1321 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
1381 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg
1441 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa
1501 gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta
1561 tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac tagtccagtg
1621 tggtggaatt cccatggaag aacgaaagta gtataaaagt aataaaacaa aaaaaagaat
1681 ataaaaaatt tatagccact ttctttgagg actgttttcc tgaaggaaat gaacctctgg
1741 aattagttag atatatagaa ttagtataca cgctagatta ttctcaaact cctaattatg
1801 acagactacg tagactgttt atacaagatt gaaaatatat ttctttttat tgagtggtgg
1861 tagttacgga tatctaatat taatattaga ctatctctat cgtcacacaa caaaatcgat
1921 tgccatggat atcttcaggg aaatcgcatc ttctatgaaa ggagagaatg tattcatttc
1981 tccaccgtca atctcgtcag tattgacaat actgtattat ggagctaatg gatccactgc
2041 tgaacagcta tcaaaatatg tagaaaagga ggcggacaag aataaggatg atatctcatt
2101 caagtccatg aataaagtat atgggcgata ttctgcagtg tttaaagatt cctttttgag
2161 aaaaattgga gataatttcc aaactgttga cttcactgat tgtcgcactg tagatgcgat
2221 caacaagtgt gttgatatct tcactgaggg gaaaattaat ccactattgg atgaaccatt
2281 gtctccagat acctgtctcc tagcaattag tgccgtatac tttaaagcaa aatggttgat
2341 gccatttgaa aaggaattta ccagtgatta tcccttttac gtatctccaa cggaaatggt
2401 agatgtaagt atgatgtcta tgtacggcga ggcatttaat cacgcatctg taaaagaatc
2461 attcggcaac ttttcaatca tagaactgcc atatgttgga gatactagta tggtggtaat
2521 tcttccagac aatattgatg gactagaatc catagaacaa aatctaacag atacaaattt
2581 taagaaatgg tgtgactcta tggatgctat gtttatcgat gtgcacattc ccaagtttaa
2641 ggtaacaggc tcgtataatc tggtggatgc gctagtaaag ttgggactga cagaggtgtt
2701 cggttcaact ggagattata gcaatatgtg taattcagat gtgagtgtcg acgctatgat
2761 ccacaaaacg tatatagatg tcaatgaaga gtatacagaa gcagctgcag caacttgtgc
2821 gctggtggca gactgtgcat caacagttac aaatgagttc tgtgcagatc atccgttcat
2881 ctatgtgatt aggcatgtcg atggcaaaat tcttttcgtt ggtagatatt gctctccaac
2941 aactaattaa atcacattct taatattaga atattagaat attatatagt taagattttt
3001 actaattggt taaccatttt tttaaaaaaa tagaaaaaaa acatgttata ttagcgaggg
3061 tcgttattct tccaattgca attggtaaga tgacggggaa ttctgcagat atccagcaca
3121 gtggcggccg ctcgagtcta gagggccctt cgaacaaaaa ctcatctcag aagaggatct
3181 gaatatgcat accggtcatc atcaccatca ccattgagtt taaacccgct gatcagcctc
3241 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
3301 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
3361 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
3421 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
3481 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
3541 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
3601 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
3661 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
3721 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
3781 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
3841 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
3901 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
3961 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
4021 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
4081 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
4141 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
4201 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
4261 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
4321 gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca
4381 aggtgaggaa ctaaaccatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa
4441 gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag
4501 ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac
4561 cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt
4621 gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac
4681 aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc
4741 cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggctaagcac
4801 ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc
4861 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
4921 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
4981 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
5041 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
5101 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
5161 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
5221 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
5281 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
5341 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
5401 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
5461 aagaacatgt gagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
5521 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
5581 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
5641 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
5701 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
5761 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
5821 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
5881 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
5941 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
6001 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
6061 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
6121 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
6181 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
6241 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
6301 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
6361 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
6421 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
6481 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
6541 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
6601 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
6661 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
6721 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
6781 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
6841 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
6901 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
6961 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
7021 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
7081 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
7141 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
7201 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
7261 acctgacgtc
//