LOCUS LT727085 7628 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pEF1crmA-T291R, complete sequence.
ACCESSION LT727085
VERSION LT727085.1
KEYWORDS .
SOURCE Mammalian expression vector pEF1crmA-T291R
ORGANISM Mammalian expression vector pEF1crmA-T291R
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7628)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..7628
/organism="Mammalian expression vector pEF1crmA-T291R"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5104."
/db_xref="taxon:1944974"
regulatory 508..1651
/note="hEF1alpha promoter"
/regulatory_class="promoter"
misc_feature 704..1642
/note="intron"
regulatory 1668..1684
/note="T7 promoter"
/regulatory_class="promoter"
misc_feature 1754..2028
/note="5' UTR of crmA"
CDS 2029..3054
/transl_table=11
/note="crmA-T291R"
/protein_id="SJL87388.1"
/translation="MDIFREIASSMKGENVFISPPSISSVLTILYYGANGSTAEQLSK
YVEKEADKNKDDISFKSMNKVYGRYSAVFKDSFLRKIGDNFQTVDFTDCRTVDAINKC
VDIFTEGKINPLLDEPLSPDTCLLAISAVYFKAKWLMPFEKEFTSDYPFYVSPTEMVD
VSMMSMYGEAFNHASVKESFGNFSIIELPYVGDTSMVVILPDNIDGLESIEQNLTDTN
FKKWCDSMDAMFIDVHIPKFKVTGSYNLVDALVKLGLTEVFGSTGDYSNMCNSDVSVD
AMIHKTYIDVNEEYREAAAATCALVADCASTVTNEFCADHPFIYVIRHVDGKILFVGR
YCSPTTN"
misc_feature 3055..3200
/note="3' UTR of crmA"
misc_feature 3259..3300
/note="V5 epitope"
misc_feature 3310..3327
/note="His-tag"
regulatory 3356..3580
/note="bovine growth hormone polyA"
/regulatory_class="terminator"
rep_origin 3626..4054
/note="f1 ori"
regulatory 4059..4340
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 4297..4372
/note="SV40 ori"
CDS 4464..5258
/transl_table=11
/note="neomycin"
/protein_id="SJL87389.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
regulatory 5434..5508
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(5434..5508)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(5822..6213)
/note="pMB1 ori"
CDS complement(6754..7614)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87390.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1814 a 1839 c 1968 g 2007 t
ORIGIN
1 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
61 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
121 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
181 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
241 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
301 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
361 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga
421 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg
481 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
541 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
601 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
661 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
721 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
781 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
841 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
901 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
961 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
1021 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
1081 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
1141 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
1201 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
1261 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
1321 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
1381 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
1441 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
1501 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
1561 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
1621 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac
1681 tatagggaga cccaagctgg ctaggtaagc ttggtaccga gctcggatcc actagtccag
1741 tgtggtggaa ttcccatgga agaacgaaag tagtataaaa gtaataaaac aaaaaaaaga
1801 atataaaaaa tttatagcca ctttctttga ggactgtttt cctgaaggaa atgaacctct
1861 ggaattagta tacacgctag attattctca aactcctaat tatgacagac tacgtagact
1921 gtttatacaa gattgaaaat atatttcttt ttattgagtg gtggtagtta cggatatcta
1981 atattaatat tagactatct ctatcgtcac acaacaaaat cgattgccat ggatatcttc
2041 agggaaatcg catcttctat gaaaggagag aatgtattca tttctccacc gtcaatctcg
2101 tcagtattga caatactgta ttatggagct aatggatcca ctgctgaaca gctatcaaaa
2161 tatgtagaaa aggaggcgga caagaataag gatgatatct cattcaagtc catgaataaa
2221 gtatatgggc gatattctgc agtgtttaaa gattcctttt tgagaaaaat tggagataat
2281 ttccaaactg ttgacttcac tgattgtcgc actgtagatg cgatcaacaa gtgtgttgat
2341 atcttcactg aggggaaaat taatccacta ttggatgaac cattgtctcc agatacctgt
2401 ctcctagcaa ttagtgccgt atactttaaa gcaaaatggt tgatgccatt tgaaaaggaa
2461 tttaccagtg attatccctt ttacgtatct ccaacggaaa tggtagatgt aagtatgatg
2521 tctatgtacg gcgaggcatt taatcacgca tctgtaaaag aatcattcgg caacttttca
2581 atcatagaac tgccatatgt tggagatact agtatggtgg taattcttcc agacaatatt
2641 gatggactag aatccataga acaaaatcta acagatacaa attttaagaa atggtgtgac
2701 tctatggatg ctatgtttat cgatgtgcac attcccaagt ttaaggtaac aggctcgtat
2761 aatctggtgg atgcgctagt aaagttggga ctgacagagg tgttcggttc aactggagat
2821 tatagcaata tgtgtaattc agatgtgagt gtcgacgcta tgatccacaa aacgtatata
2881 gatgtcaatg aagagtatcg cgaagcggct gcagcaactt gtgcgctggt ggcagactgt
2941 gcatcaacag ttacaaatga gttctgtgca gatcatccgt tcatctatgt gattaggcat
3001 gtcgatggca aaattctttt cgttggtaga tattgctctc caacaactaa ttaaatcaca
3061 ttcttaatat tagaatatta gaatattata tagttaagat ttttactaat tggttaacca
3121 tttttttaaa aaaatagaaa aaaaacatgt tatattagcg agggtcgtta ttcttccaat
3181 tgcaattggt aagatgacgg ggaattctgc agatatccag cacagtggcg gccgctcgag
3241 tctagagggc ccttcgaagg taagcctatc cctaaccctc tcctcggtct cgattctacg
3301 cgtaccggtc atcatcacca tcaccattga gtttaaaccc gctgatcagc ctcgactgtg
3361 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
3421 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
3481 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
3541 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
3601 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
3661 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
3721 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
3781 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
3841 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
3901 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
3961 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
4021 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
4081 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
4141 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
4201 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
4261 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
4321 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
4381 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag
4441 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
4501 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
4561 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
4621 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
4681 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
4741 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
4801 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
4861 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
4921 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
4981 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
5041 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
5101 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
5161 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
5221 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgcgaa
5281 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
5341 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
5401 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
5461 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
5521 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
5581 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
5641 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
5701 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
5761 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
5821 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
5881 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
5941 aagaacatgt gagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
6001 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
6061 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
6121 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
6181 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
6241 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
6301 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
6361 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
6421 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
6481 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
6541 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
6601 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
6661 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
6721 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
6781 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
6841 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
6901 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
6961 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
7021 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
7081 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
7141 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
7201 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
7261 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
7321 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
7381 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
7441 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
7501 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
7561 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
7621 cctttttc
//