LOCUS LT727076 5872 bp DNA circular SYN 06-FEB-2017
DEFINITION Bacterial expression vector pGEXGSTp65(12-317)S276C, complete
sequence.
ACCESSION LT727076
VERSION LT727076.1
KEYWORDS .
SOURCE Bacterial expression vector pGEXGSTp65(12-317)S276C
ORGANISM Bacterial expression vector pGEXGSTp65(12-317)S276C
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5872)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5872
/organism="Bacterial expression vector
pGEXGSTp65(12-317)S276C"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5056."
/db_xref="taxon:1944670"
CDS complement(<7..447)
/transl_table=11
/note="lacZ fragment, incl. lacZalpha"
/protein_id="SJL87359.1"
/translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGV"
misc_feature complement(448..485)
/note="5' UTR of lacZ, incl. RBS"
regulatory complement(486..569)
/note="lac promoter"
/regulatory_class="promoter"
CDS complement(570..1652)
/transl_table=11
/note="lacI"
/protein_id="SJL87360.1"
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
QTASPRALADSLMQLARQVSRLESGQ"
regulatory complement(1653..1730)
/note="lacIq promoter, incl. RBS"
/regulatory_class="promoter"
rep_origin complement(1790..2192)
/note="pMB1 ori"
CDS complement(2733..3593)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87361.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
CDS complement(<4020..>4937)
/codon_start=1
/transl_table=11
/note="hNF-kappaB p65 mutated fragment"
/protein_id="SJL87362.1"
/translation="EPAQASGPYVEIIEQPKQRGMRFRYKCEGRSAGSIPGERSTDTT
KTHPTIKINGYTGPGTVRISLVTKDPPHRPHPHELVGKDCRDGFYEAELCPDRCIHSF
QNLGIQCVKKRDLEQAISQRIQTNNNPFQVPIEEQRGDYDLNAVRLCFQVTVRDPSGR
PLRLPPVLPHPIFDNRAPNTAELKICRVNRNSGSCLGGDEIFLLCDKVQKEDIEVYFT
GPGWEARGSFSQADVHRQVAIVFRTPPYADPSLQAPVRVSMQLRRPCDRELSEPMEFQ
YLPDTDDRHRIEEKRKRTYETFKSIMKKSP"
misc_feature complement(4143..4145)
/note="S276C"
misc_feature complement(4938..4955)
/note="thrombin recognition site"
CDS complement(<4962..5615)
/transl_table=11
/note="GST"
/protein_id="SJL87363.1"
/translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNK
KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDI
RYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALD
VVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK
"
misc_feature complement(5621..5656)
/note="5' UTR of lacZ, incl. RBS"
regulatory complement(5657..5699)
/note="tac promoter"
/regulatory_class="promoter"
BASE COUNT 1412 a 1543 c 1482 g 1435 t
ORIGIN
1 aattccaacg ccatcaaaaa taattcgcgt ctggccttcc tgtagccagc tttcatcaac
61 attaaatgtg agcgagtaac aacccgtcgg attctccgtg ggaacaaacg gcggattgac
121 cgtaatggga taggttacgt tggtgtagat gggcgcatcg taaccgtgca tctgccagtt
181 tgaggggacg acgacagtat cggcctcagg aagatcgcac tccagccagc tttccggcac
241 cgcttctggt gccggaaacc aggcaaagcg ccattcgcca ttcaggctgc gcaactgttg
301 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
361 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
421 ggccagtgaa tccgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
481 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
541 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
601 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
661 gccagggtgg tttttctttt caccagtgag acgggcaaca gctgattgcc cttcaccgcc
721 tggccctgag agagttgcag caagcggtcc acgctggttt gccccagcag gcgaaaatcc
781 tgtttgatgg tggttaacgg cgggatataa catgagctgt cttcggtatc gtcgtatccc
841 actaccgaga tatccgcacc aacgcgcagc ccggactcgg taatggcgcg cattgcgccc
901 agcgccatct gatcgttggc aaccagcatc gcagtgggaa cgatgccctc attcagcatt
961 tgcatggttt gttgaaaacc ggacatggca ctccagtcgc cttcccgttc cgctatcggc
1021 tgaatttgat tgcgagtgag atatttatgc cagccagcca gacgcagacg cgccgagaca
1081 gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc
1141 acgcccagtc gcgtaccgtc ttcatgggag aaaataatac tgttgatggg tgtctggtca
1201 gagacatcaa gaaataacgc cggaacatta gtgcaggcag cttccacagc aatggcatcc
1261 tggtcatcca gcggatagtt aatgatcagc ccactgacgc gttgcgcgag aagattgtgc
1321 accgccgctt tacaggcttc gacgccgctt cgttctacca tcgacaccac cacgctggca
1381 cccagttgat cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc
1441 agactggagg tggcaacgcc aatcagcaac gactgtttgc ccgccagttg ttgtgccacg
1501 cggttgggaa tgtaattcag ctccgccatc gccgcttcca ctttttcccg cgttttcgca
1561 gaaacgtggc tggcctggtt caccacgcgg gaaacggtct gataagagac accggcatac
1621 tctgcgacat cgtataacgt tactggtttc acattcacca ccctgaattg actctcttcc
1681 gggcgctatc atgccatacc gcgaaaggtt ttgcaccatt cgatggtgtc ggaatttatg
1741 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
1801 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
1861 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
1921 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
1981 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
2041 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
2101 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
2161 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
2221 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
2281 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
2341 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
2401 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
2461 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
2521 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
2581 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
2641 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
2701 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
2761 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
2821 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
2881 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
2941 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
3001 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg
3061 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
3121 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
3181 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
3241 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
3301 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat
3361 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
3421 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
3481 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
3541 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
3601 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
3661 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
3721 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
3781 aggccctttc gtcttcaaga attatacact ccgctatcgc tacgtgactg ggtcatggct
3841 gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
3901 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
3961 tcatcaccga aacgcgcgag gcagatcgtc agtcagtcac gatgaattcc cggggatcca
4021 ggactcttct tcatgatgct cttgaaggtc tcatatgtcc ttttacgttt ctcctcaatc
4081 cggtgacgat cgtctgtatc tggcaggtac tggaattcca tgggctcact gagctcccgg
4141 tcgcaaggcc tccgcagctg catggagaca cgcacaggag cctgcaggct ggggtctgcg
4201 tagggagggg tccggaacac aatggccact tgtcggtgca catcagcttg cgaaaaggag
4261 cctcgggcct cccagcctgg tcccgtgaaa tacacctcaa tgtcctcttt ctgcaccttg
4321 tcacacagta ggaagatctc atccccaccg aggcagctgc cagagtttcg gttcactcgg
4381 cagatcttga gctcggcagt gttgggggca cgattgtcaa agatgggatg aggaaggaca
4441 ggcggcaggc ggaggggcct gcctgatggg tcccgcactg tcacctggaa gcagagccgc
4501 acagcattca ggtcgtagtc cccacgctgc tcttctatag gaacttggaa ggggttgttg
4561 ttggtctgga tgcgctgact gatagcctgc tccaggtccc gcttcttcac acactggatt
4621 cccaggttct ggaaactgtg gatgcagcgg tccgggcaga gctcagcctc atagaagcca
4681 tcccggcagt cctttcctac aagctcgtgg gggtgaggcc ggtgaggagg gtccttggtg
4741 accagggaga tgcgcactgt ccctggtcct gtgtagccat tgatcttgat ggtggggtgg
4801 gtcttggtgg tatctgtgct cctctcgcct gggatgctgc ccgcggagcg cccctcgcac
4861 ttgtagcgga agcgcatgcc ccgctgcttg ggctgctcaa tgatctccac ataggggcca
4921 gaggcctggg ctggctcgga tccacgcgga accagatccg attttggagg atggtcgcca
4981 ccaccaaacg tggcttgcca gccctgcaaa ggccatgcta tatacttgct ggatttcaag
5041 tacttatcaa tttgtgggat agcttcaata cgttttttaa aacaaactaa ttttgggaac
5101 gcatccaggc acattgggtc catgtataaa acaacatcaa gagcgtcata caacatgaag
5161 tcaggatggg ttacatgatc accatttaaa tatgttttat gacataaacg atcttcgaac
5221 attttcagca tttcaggtag cttgctaaga aaatcaactt tgagagtttc aaagtcttta
5281 ctatatgcaa ttctcgaaac accgtatcta atatccaaaa ccgctccttc aagcattgaa
5341 atctctgcac gctcttttgg acaaccaccc aacatgttgt gcttgtcagc tatataacgt
5401 atgatggcca tagactgtgt taatttaaca tcaccatcaa tataataagg aagattggga
5461 aactccaaac ccaattcaaa ctttttgttt cgccatttat caccttcatc gcgctcatac
5521 aaatgctctt catatttttc ttcaagatat tccaaaagaa gtcgagtggg ttgcacaagg
5581 cccttaattt tccaataacc tagtataggg gacatgaata ctgtttcctg tgtgaaattg
5641 ttatccgctc acaattccac acattatacg agccgatgat taattgtcaa cagctcattt
5701 cagaatattt gccagaaccg ttatgatgtc ggcgcaaaaa acattatcca gaacgggagt
5761 gcgccttgag cgacacgaat tatgcagtga tttacgacct gcacagccat accacagctt
5821 ccgatggctg cctgacgcca gaagcattgg tgcaccgtgc agtcgataac gt
//