LOCUS LT727074 4020 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pCAT3, complete sequence.
ACCESSION LT727074
VERSION LT727074.1
KEYWORDS .
SOURCE Vector pCAT3
ORGANISM Vector pCAT3
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4020)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..4020
/organism="Vector pCAT3"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5034."
/db_xref="taxon:1945275"
rep_origin complement(3..458)
/note="f1 ori"
regulatory 626..642
/note="T7"
/regulatory_class="promoter"
misc_feature complement(716..890)
/note="3UTR"
CDS complement(891..>1774)
/codon_start=3
/transl_table=11
/note="CAT3f"
/protein_id="SJL87352.1"
/translation="RHMEGSGVNTYMLINKAGKAHYVKFHWKPTCGVKCLLEEEAIKV
GGANHSHATKDLYDSIAAGNYPEWKLFIQIIDPDHEDRFDFDPLDVTKTWPEDILPLQ
PVGRLVLNKNIDNFFAENEQLAFWPAIVVPGVYCSDDKLLQTRIFSYSDAQRHRLGPN
YLQLPVNAPKCAHHNNHHEGFMNFMHRDEEVNYFPSRFDPCRHAEQYPIPPCVLTGKR
DKCIIEKENNFKQPGERYRSWAPDRQERFICRWVDALSDPRVTHEIRSIWFSYWSQAD
KTLGQKIASRLNVRPTM"
regulatory complement(1834..1850)
/note="T3"
/regulatory_class="promoter"
regulatory complement(1914..1997)
/note="lac"
/regulatory_class="promoter"
rep_origin complement(2089..2491)
/note="pMB1 ori"
CDS complement(3032..3892)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87353.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1046 a 979 c 978 g 1017 t
ORIGIN
1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
661 ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcaggaat tcgatacttg
721 aacaaagtac ttcaacttta ttcgggcaac aacgtcagaa taacacaaga aagtttgttt
781 ggtacaacat acaacccaat caccaaaaca gtattctctc caaacaaaca ctgatttctt
841 ggattaatct ttcagcaaca ggaacatatt tgaatatact actatcagct tcacattgtg
901 ggcctgacat tgagacggga cgcgatcttc tgaccaagag ttttgtcagc ctgtgaccag
961 tatgaaaacc agatactgcg tatctcatga gtgactcgag gatcggatag agcatcaacc
1021 catctgcaga taaatctttc ttgcctgtcc ggtgcccaag atctgtatct ctctcccggc
1081 tgcttgaagt tgttctcttt ttcaatgatg cacttgtcac gttttcctgt caagacacaa
1141 ggaggaattg gatactgttc tgcatgacga caaggatcaa accttgaggg gaaatagttg
1201 acctcttcat cgcggtgcat gaaattcatg aaaccttcat ggtgattatt gtgatgagca
1261 cacttgggag cgttaactgg gagctgcaga tagtttggcc caagacggtg tctctgggca
1321 tcagaatatg agaatatcct agtctggaga agcttgtcat ccgaacagta aacaccaggg
1381 acaacaatgg caggccaaaa ggcaagctgt tcattctctg caaagaagtt atcgatattc
1441 ttattcaata ccaagcgacc aactggctgc aatggcaaga tgtcctcagg ccaggtcttg
1501 gttacatcta gggggtcaaa gtcaaatctg tcttcatgat caggatcgat aatttggatg
1561 aacagtttcc actctggata gtttccagca gcaattgaat cgtagagatc cttggtggcg
1621 tgactatgat tcgctcctcc taccttaata gcttcttcct ccaacaagca cttgactcca
1681 caagttggct tccaatggaa tttcacataa tgtgctttcc cagctttgtt gatcaacatg
1741 taggtattaa caccggaacc ttccatgtgc ctgtatcaag cttatcgata ccgtcgacct
1801 cgaggggggg cccggtaccc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg
1861 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
1921 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
1981 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
2041 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
2101 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
2161 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
2221 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
2281 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
2341 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
2401 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
2461 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
2521 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
2581 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
2641 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
2701 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
2761 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
2821 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
2881 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
2941 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
3001 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
3061 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
3121 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
3181 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
3241 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
3301 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
3361 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
3421 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
3481 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
3541 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
3601 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
3661 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
3721 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
3781 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
3841 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
3901 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
3961 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
//