LOCUS LT727073 4048 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pCAT2, complete sequence.
ACCESSION LT727073
VERSION LT727073.1
KEYWORDS .
SOURCE Vector pCAT2
ORGANISM Vector pCAT2
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4048)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..4048
/organism="Vector pCAT2"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5033."
/db_xref="taxon:1945274"
rep_origin complement(3..458)
/note="f1 ori"
regulatory 626..642
/note="T7"
/regulatory_class="promoter"
misc_feature complement(716..907)
/note="3UTR"
CDS complement(908..>1802)
/codon_start=2
/transl_table=11
/note="CAT2f"
/protein_id="SJL87350.1"
/translation="PINYRHMEGYGVHAYQLINKAGKAHYVKFHWKPTCGVKCMTEEE
AIRVGGTNHSHATKDLYDSIAVGNYPEWKLSIHIMDPEDVERFDFDPLDVTKIWPEDI
LPLMPVGRLVLNRNIDNFFAENEQLAFNPGHIVPGLYYSEDKLLQTRIFAYADTQRHR
IGPNYMQLPVNAPKCAHHNNHRDGAMNFMHRDEEVDYLPSRFDPCRHAEQYPIPSRVL
NGRREMCVIEKENNFKQAGERYRSWEPDRQDRYVSKWVEHLSDPRVTYEIRSIWISYL
SQADKSCGQKVASRLTLKPTM"
regulatory complement(1862..1878)
/note="T3"
/regulatory_class="promoter"
regulatory complement(1942..2025)
/note="lac"
/regulatory_class="promoter"
rep_origin complement(2117..2519)
/note="pMB1 ori"
CDS complement(3060..3920)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87351.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1053 a 986 c 977 g 1032 t
ORIGIN
1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
661 ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcaggaat tcgatacctt
721 attcaagtgc taatatatat tgtgtcatag taaacactgg atatccaagt ttggtacaag
781 cagaacataa aaagaaacag tacactatag caaaaacaca taaacgtaat gcttttcttg
841 gtttagtagt actccttcaa actgcaactt gagacgtttt cccagtagtg ttttcatctt
901 agcttcatca cattgtaggc tttaaagtga gacgagaagc gaccttctga ccacaagact
961 tgtcagcctg agacaagtat gatatccata tactgcgtat ctcataagtg actcggggat
1021 cagataaatg ctcaacccat ttgctaacgt atctgtcttg cctgtcaggt tcccaggatc
1081 tgtatctctc tcctgcctgc ttgaagttgt tctctttctc aatgacacac atttcacgcc
1141 ttccattcaa gacacgagaa ggaattgggt actgttcagc atgacgacaa ggatcgaacc
1201 ttgagggcaa ataatccacc tcttcatcgc gatgcatgaa gttcatggca ccatcgcggt
1261 gattattgtg atgagcacac ttgggagcat taacaggaag ctgcatatag tttggtccaa
1321 tacggtgtct ctgagtatca gcatacgcga atatccttgt ctggagaagc ttatcctccg
1381 aatagtacag accagggaca atatggccag ggttaaacgc gagctgctcg ttctcagcaa
1441 agaagttatc gatattcctg ttaagtacca atcgaccaac tggcatcaat ggcaagatat
1501 cctcaggcca gatcttggtt acatcaagag gatcaaagtc gaatctttca acatcctcag
1561 ggtccataat gtggatagag agtttccact cgggatagtt tccaacagca atcgaatcgt
1621 acagatcctt ggtggcgtga ctatgatttg tacctccgac cctaatagct tcttcctctg
1681 tcatgcactt gacaccacaa gttggcttcc agtgaaactt cacataatgt gcttttccag
1741 ccttgttgat taattgatag gcgtggacac cataaccttc catgtgtctg taatttatcg
1801 ggatcaagct tatcgatacc gtcgacctcg agggggggcc cggtacccag cttttgttcc
1861 ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga
1921 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
1981 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
2041 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
2101 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
2161 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
2221 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
2281 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
2341 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
2401 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
2461 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
2521 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
2581 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
2641 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
2701 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
2761 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
2821 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
2881 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
2941 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
3001 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
3061 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
3121 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
3181 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
3241 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
3301 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
3361 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
3421 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
3481 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
3541 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
3601 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
3661 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
3721 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
3781 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
3841 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
3901 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
3961 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
4021 ccgcgcacat ttccccgaaa agtgccac
//