LOCUS LT727072 4025 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pCAT1, complete sequence.
ACCESSION LT727072
VERSION LT727072.1
KEYWORDS .
SOURCE Vector pCAT1
ORGANISM Vector pCAT1
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4025)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..4025
/organism="Vector pCAT1"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5032."
/db_xref="taxon:1945273"
rep_origin complement(3..458)
/note="f1 ori"
regulatory 626..642
/note="T7"
/regulatory_class="promoter"
CDS <716..1545
/codon_start=3
/transl_table=11
/note="CAT1f"
/protein_id="SJL87348.1"
/translation="KSTYVKFHWKPTCGVKSLLEDEAARVGGANHSHATQDLYDSIAA
GNYPEWKLFIQTMDPDHEDRFDFDPLDVTKTWPEDILPLQPVGRLVLNKNIDNFSNEN
EQLAFCPSIVVPGVYYSDDKMLQTRIFSYSDTQRYRLGPNYLQLPANAPKCAHHNNHY
DGSMNFMHRDEEIDYFPSRYDPVRHAEKYPIPSTMCTGKREKCVIQKENNFKQPGERY
RSFTPDRQERFIRRWVETLSDPRITYEIRSIWISYWSQADKSLGQKLASRLNVRPSI"
misc_feature 1546..1779
/note="3UTR"
regulatory complement(1839..1855)
/note="T3"
/regulatory_class="promoter"
regulatory complement(1919..2002)
/note="lac"
/regulatory_class="promoter"
rep_origin complement(2094..2496)
/note="pMB1 ori"
CDS complement(3037..3897)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87349.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1059 a 953 c 987 g 1026 t
ORIGIN
1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
661 ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcaggaat tcgatggaaa
721 tcaacctatg tgaagttcca ctggaagccc acatgtggtg tcaagtcctt gttggaagat
781 gaggcagcac gagttggagg agcaaatcac agccacgcta ctcaggacct ctatgactct
841 attgctgctg gaaattatcc tgaatggaag ctcttcattc agactatgga tccagatcat
901 gaagacagat ttgattttga tccacttgat gtgacaaaaa cttggccaga ggatatcttg
961 cctttgcagc cggtgggaag attagttctg aacaagaaca ttgataactt ctctaatgag
1021 aatgagcagc tagctttctg cccttctatt gttgttccag gggtttatta ctcagatgat
1081 aagatgcttc aaactcgtat tttctcctac tctgataccc agaggtatcg acttggacca
1141 aactatttgc aactacctgc taatgctcca aaatgcgctc atcacaacaa tcactacgat
1201 ggctccatga attttatgca cagggatgag gagatcgact acttcccttc aaggtatgat
1261 cctgttcgcc atgctgagaa gtatcctatt ccttctacaa tgtgcactgg caaacgcgag
1321 aagtgtgtca ttcagaaaga gaacaatttc aagcaaccag gagaaaggta ccgctcattc
1381 acaccagaca ggcaagaacg ctttattcgt cggtgggtgg agaccttgtc tgatcctcgt
1441 atcacttatg agatccgcag catttggatc tcatactggt ctcaggctga caaatctctg
1501 ggtcaaaagc tagcttctag gcttaatgtg agaccaagca tatgaagatg aagcttttaa
1561 tggtttcgga ggaggtgatc taaatgcgag agtctcaagg cattaataac tgatacccat
1621 tccagtcata taagcaagat gtataatgtg gtggcgatca tattaagatg aaataaatag
1681 agaattccta atatatgtgc attcattatt gtgttgctgt tatatgaaca cgagtgaaac
1741 aatggaagtt atagataata tttcttttct tgtgctatta tcaagcttat cgataccgtc
1801 gacctcgagg gggggcccgg tacccagctt ttgttccctt tagtgagggt taattgcgcg
1861 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
1921 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
1981 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
2041 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
2101 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
2161 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
2221 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
2281 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
2341 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
2401 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
2461 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
2521 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
2581 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
2641 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
2701 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
2761 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
2821 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
2881 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
2941 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
3001 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
3061 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
3121 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
3181 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
3241 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
3301 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
3361 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
3421 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
3481 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
3541 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
3601 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
3661 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
3721 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
3781 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
3841 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
3901 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
3961 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
4021 gccac
//