LOCUS LT727066 10195 bp DNA circular SYN 06-FEB-2017 DEFINITION Yeast expression vector pEMBLyex-kanMX4, complete sequence. ACCESSION LT727066 VERSION LT727066.1 KEYWORDS . SOURCE Yeast expression vector pEMBLyex-kanMX4 ORGANISM Yeast expression vector pEMBLyex-kanMX4 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 10195) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..10195 /organism="Yeast expression vector pEMBLyex-kanMX4" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 5011." /db_xref="taxon:1945468" rep_origin complement(754..1209) /note="f1 ori" CDS complement(1977..3071) /transl_table=11 /note="defective LEU2" /protein_id="SJL87331.1" /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENH LIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQL YANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSE QYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLK VQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPD KNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLD AGIRTGDLGGSNSTTEVGDAVAEEVKKILA" rep_origin complement(3429..4409) /note="2mu ori" misc_feature 4176..4778 /note="2mu inverted repeat" regulatory complement(4491..4595) /note="FLP terminator" /regulatory_class="terminator" regulatory complement(4909..5316) /note="GAL10/CYC1 promoter" /regulatory_class="promoter" CDS complement(5535..6338) /transl_table=11 /note="URA3" /protein_id="SJL87332.1" /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN" rep_origin complement(6793..7195) /note="pMB1 ori" CDS complement(7735..8595) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL87333.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" regulatory complement(8734..8931) /note="TEF1 terminator" /regulatory_class="terminator" CDS complement(8937..9746) /transl_table=11 /note="Tn903 neomycin resistance" /protein_id="SJL87334.1" /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGK PDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVD ASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRV GIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" regulatory complement(9747..10090) /note="TEF1 promoter" /regulatory_class="promoter" BASE COUNT 2907 a 2264 c 2199 g 2814 t ORIGIN 1 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 61 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 121 gggtgttggc gggtgtcggg gcgcagccat nnnccannna cgtagcgata gcggagtnta 181 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt 241 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggnaattcca acatccaata 301 aatcatacag gcaaggcaaa gaattagcaa aattaagcaa taaagcctca gagcataaag 361 ctaaatcggt tgtaccaaaa acattatgac cctgtaatac ttttgcggga gaagccttta 421 tttcaacgca aggataaaaa tttttagaac cctcatatat tttaaatgca atgcctgagt 481 aatgtgtagg taaagattca aacgggtgag aaaggccgga gacagtcaaa tcaccatcaa 541 tatgatattc aaccgttcta gctgataaat tcatgccgga gagggtagct atttttgaga 601 ggtctacaaa ggctatcagg tcattgcctg agagtctgga gcaaacaaga gaatcgatga 661 acggtaatcg taaaactagc atgtcaatca tatgtacccc ggttgataat cagaaaagcc 721 ccaaaaacag gaagattgta taagcaaata tttaaattgt aaacgttaat attttgttaa 781 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca 841 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga 901 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc 961 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc 1021 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc 1081 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg 1141 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac 1201 agggcgcgta ctatggttgc tttgacgagc acgtataacg tgctttcctc gttagaatca 1261 gagcgggagc taaacaggag gccgattaaa gggattttag acaggaacgg tacgccagaa 1321 tcctgagaag tgtttttata atcagtgagg ccaccgagta aaagagtctg tccatcacgc 1381 aaattaaccg ttgtcgcaat acttctttga ttagtaataa catcacttgc ctgagtagaa 1441 gaactcaaac tatcggcctt gctggtaata tccagaacaa tattaccgcc agccattgca 1501 acaggaaaaa cgctcatgga aatacctaca ttttgacgct caatcgtctg gaattnccat 1561 tcgccattca ggctacgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta 1621 cgccanctgg cgaagggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt 1681 tcccagtcag tacgttgtaa aacgacggcc agtgccaagc tccttaccac tcttttgtta 1741 ctctattgat ccagctcagc aaaggcagtg tgatctaaga ttctatcttc gcgatgtagt 1801 aaaactagct agaccgagaa agagactaga aatgcaaaag gcacttctac aatggctgcc 1861 atcattatta tccgatgtga cgctgcattt tttttttttt gtaatttcgt gtcgtttcta 1921 ttatgaattt catttataaa gtttatgtac aaatatcata aaaaaagaga atctttttaa 1981 gcaaggattt tcttaacttc ttcggcgaca gcatcaccga cttcggtggt actgttggaa 2041 ccacctaaat caccagttct gatacctgca tccaaaacct ttttaactgc atcttcaatg 2101 gccttacctt cttcaggcaa gttcaatgac aatttcaaca tcattgcagc agacaagata 2161 gtggcgatag ggtcaacctt attctttggc aaatctggag cagaaccatg gcatggttcg 2221 tacaaaccaa atgcggtgtt cttgtctggc aaagaggcca aggacgcaga tggcaacaaa 2281 cccaaggaac ctgggataac ggaggcttca tcggagatga tatcaccaaa catgttgctg 2341 gtgattataa taccatttag gtgggttggg ttcttaacta ggatcatggc ggcagaatca 2401 atcaattgat gttgaacctt caatgtaggg aattcgttct tgatggtttc ctccacagtt 2461 tttctccata atcttgaaga ggccaaaaca ttagctttat ccaaggacca aataggcaat 2521 ggtggctcat gttgtagggc catgaaagcg gccattcttg tgattctttg cacttctgga 2581 acggtgtatt gttcactatc ccaagcgaca ccatcaccat cgtcttcctt tctcttacca 2641 aagtaaatac ctcccactaa ttctctgaca acaacgaagt cagtaccttt agcaaattgt 2701 ggcttgattg gagataagtc taaaagagag tcggatgcaa agttacatgg tcttaagttg 2761 gcgtacaatt gaagttcttt acggattttt agtaaacctt gttcaggtct aacactaccg 2821 gtaccccatt taggaccacc cacagcacct aacaaaacgg catcaacctt cttggaggct 2881 tccagcgcct catctggaag tgggacacct gtagcatcga tagcagcacc accaattaaa 2941 tgattttcga aatcgaactt gacattggaa cgaacatcag aaatagcttt aagaacctta 3001 atggcttcgg ctgtgatttc ttgaccaacg tggtcacctg gcaaaacgac gatcttctta 3061 ggggcagaca ttacaatggt atatccttga aatatatata aaaaaaaaaa aaaatgcagc 3121 ttctcaatga tattcgaata cgctttgagg agatacagcc taatatccga caaactgttt 3181 tacagattta cgatcgtact tgttacccat cattgaattt tgaacatccg aacctgggag 3241 ttttccctga aacagatagt atatttgaac ctgtataata atatatagtc tagcgcttta 3301 cggaagacaa tgtatgtatt tcggttcctg gagaaactat tgcatctatt gcataggtaa 3361 tcttgcacgt cgcatccccg gttcattttc tgcgtttcca tcttgcactt caatagcata 3421 tctttgttaa cgaagcatct gtgcttcatt ttgtagaaca aaaatgcaac gcgagagcgc 3481 taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgaaag 3541 cgctatttta ccaacgaaga atctgtgctt catttttgta aaacaaaaat gcaacgcgag 3601 agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc 3661 gagagcgcta ttttaccaac aaagaatcta tacttctttt ttgttctaca aaaatgcatc 3721 ccgagagcgc tatttttcta acaaagcatc ttagattact ttttttctcc tttgtgcgct 3781 ctataatgca gtctcttgat aactttttgc actgtaggtc cgttaaggtt agaagaaggc 3841 tactttggtg tctattttct cttccataaa aaaagcctga ctccacttcc cgcgtttact 3901 gattactagc gaagctgcgg gtgcattttt tcaagataaa ggcatccccg attatattct 3961 ataccgatgt ggattgcgca tactttgtga acagaaagtg atagcgttga tgattcttca 4021 ttggtcagaa aattatgaac ggtttcttct attttgtctc tatatactac gtataggaaa 4081 tgtttacatt ttcgtattgt tttcgattca ctctatgaat agttcttact acaatttttt 4141 tgtctaaaga gtaatactag agataaacat aaaaaatgta gaggtcgagt ttagatgcaa 4201 gttcaaggag cgaaaggtgg atgggtaggt tatataggga tatagcacag agatatatag 4261 caaagagata cttttgagca atgtttgtgg aagcggtatt cgcaatattt tagtagctcg 4321 ttacagtccg gtgcgttttt ggttttttga aagtgcgtct tcagagcgct tttggttttc 4381 aaaagcgctc tgaagttcct atactttcta gctagagaat aggaacttcg gaataggaac 4441 ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag 4501 ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt atatatatat acatgagaag 4561 aacggcatag tgcgtgttta tgcttaaatg cgtacttata tgcgtctatt tatgtaggat 4621 gaaaggtagt ctagtacctc ctgtgatatt atcccattcc atgcggggta tcgtatgctt 4681 ccttcagcac taccctttag ctgttctata tgctgccact cctcaattgg attagtctca 4741 tccttcaatg ctatcatttc ctttgatatt ggatcataag cttgcatgcc tgcaggtcga 4801 ctctagagga tccccgggta ccgagctcga attgatccgg taatttagtg tgtgtatttg 4861 tgtttgcgtg tctatagaag tatagtaatt tatgctacaa aggacctaat gtataaggaa 4921 agaatattta gagaaaagaa gaaaacaaga gttttatata catacagagc acatgcatgc 4981 catatgatca tgtgtcgtcg cacacatata tatatgcctg tatgtgtcag cactaaagtt 5041 gcctggccat ccacgctata tacacgcctg gcggatctgc tcgaggtcga ttagcctaaa 5101 aaaaccttct ctttggaact ttcagtaata cgcttaactg ctcattgcta tattgaagta 5161 cggattagaa gccgccgagc gggtgacagc cctccgaagg aagactctcc tccgtgcgtc 5221 ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc tcgcgccgca ctgctccgaa 5281 caataaagat tctacaatac tagcttttat ggttatgaag aggaaaaatt ggcagtaacc 5341 tggccccaca aaccttcaaa tgaacgaatc aaattaacaa ccataggatg ataatgcgat 5401 tagtttttta gccttatttc tggggtaatt aatcagcgaa gcgatgattt ttgatcgggt 5461 aataactgat ataattaaat tgaagctcta atttgtgagt ttagtataca tgcatttact 5521 tataatacag ttttttagtt ttgctggccg catcttctca aatatgcttc ccagcctgct 5581 tttctgtaac gttcaccctc taccttagca tcccttccct ttgcaaatag tcctcttcca 5641 acaataataa tgtcagatcc tgtagagacc acatcatcca cggttctata ctgttgaccc 5701 aatgcgtctc ccttgtcatc taaacccaca ccgggtgtca taatcaacca atcgtaacct 5761 tcatctcttc cacccatgtc tctttgagca ataaagccga taacaaaatc tttgtcgctc 5821 ttcgcaatgt caacagtacc cttagtatat tctccagtag atagggagcc cttgcatgac 5881 aattctgcta acatcaaaag gcctctaggt tcctttgtta cttcttctgc cgcctgcttc 5941 aaaccgctaa caatacctgg gcccaccaca ccgtgtgcat tcgtaatgtc tgcccattct 6001 gctattctgt atacacccgc agagtactgc aatttgactg tattaccaat gtcagcaaat 6061 tttctgtctt cgaagagtaa aaaattgtac ttggcggata atgcctttag cggcttaact 6121 gtgccctcca tggaaaaatc agtcaagata tccacatgtg tttttagtaa acaaattttg 6181 ggacctaatg cttcaactaa ctccagtaat tccttggtgg tacgaacatc caatgaagca 6241 cacaagtttg tttgcttttc gtgcatgata ttaaatagct tggcagcaac aggactagga 6301 tgagtagcag cacgttcctt atatgtagct ttcgacatga tttatcttcg tttccggttt 6361 ttgttctgtg cagttgggtt aagaatactg ggcaatttca tgtttcttca acactacata 6421 tgcgtatata taccaatcta agtctgtgct ccttccttcg ttcttccttc tgttcggaga 6481 ttaccgaatc aaaaaaattt caaagaaacc gaaatcaaaa aaaagaataa aaaaaaaatg 6541 atgaattgaa ttgaaaagct aattcgtaat catggtcata gctgtttcct gtgtgaaatt 6601 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 6661 gtgcctaatg agtgaggtaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 6721 cgggaaacct gtcgtgccan ctggattaat gaatcggcca acgcgcgggg agaggcggtt 6781 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 6841 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 6901 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 6961 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 7021 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 7081 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 7141 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 7201 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 7261 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 7321 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 7381 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 7441 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 7501 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 7561 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 7621 gttagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 7681 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 7741 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 7801 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 7861 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 7921 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 7981 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 8041 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 8101 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 8161 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 8221 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 8281 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 8341 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 8401 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 8461 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 8521 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 8581 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 8641 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 8701 cacatttccc cgaaaagtgc cacctgacgt cagcagtata gcgaccagca ttcacatacg 8761 attgacgcat gatattactt tctgcgcact taacttcgca tctgggcaga tgatgtcgag 8821 gcgaaaaaaa atataaatca cgctaacatt tgattaaaat agaacaacta caatataaaa 8881 aaactataca aatgacaagt tcttgaaaac aagaatcttt ttattgtcag tactgattag 8941 aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca 9001 tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg 9061 atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt 9121 aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa 9181 tccggtgaga atggcaaaag cttatgcatt tctttccaga cttgttcaac aggccagcca 9241 ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc 9301 tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc 9361 aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct 9421 tctaatacct ggaatgctgt tttgccgggg atcgcagtgg tgagtaacca tgcatcatca 9481 ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt 9541 ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac 9601 tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta 9661 tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa tcgcggcctc 9721 gaaacgtgag tcttttcctt acccatggtt gtttatgttc ggatgtgatg tgagaactgt 9781 atcctagcaa gattttaaaa ggaagtatat gaaagaagaa cctcagtggc aaatcctaac 9841 cttttatatt tctctacagg ggcgcggcgt ggggacaatt caacgcgtct gtgaggggag 9901 cgtttccctg ctcgcaggtc tgcagcgagg agccgtaatt tttgcttcgc gccgtgcggc 9961 catcaaaatg tatggatgca aatgattata catggggatg tatgggctaa atgtacgggc 10021 gacagtcaca tcatgcccct gagctgcgca cgtcaagact gtcaaggagg gtattctggg 10081 cctccatgtc gctggccggg tgacccggcg gggacgaggc gacgtctaag aaaccattat 10141 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaa //