LOCUS LT727064 6543 bp DNA circular SYN 06-FEB-2017
DEFINITION Shuttle vector pRM2, complete sequence.
ACCESSION LT727064
VERSION LT727064.1
KEYWORDS .
SOURCE Shuttle vector pRM2
ORGANISM Shuttle vector pRM2
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6543)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6543
/organism="Shuttle vector pRM2"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4984."
/db_xref="taxon:1945228"
rep_origin complement(63..763)
/note="ARS2"
CDS complement(1662..2474)
/transl_table=11
/note="URA3"
/protein_id="SJL87326.1"
/translation="MTVNTKTYSERAETHASPVAQRLFRLMELKKTNLCASIDVDTTK
EFLELIDKLGPYVCLIKTHIDIINDFSYESTIEPLLELSRKHQFMIFEDRKFADIGNT
VKKQYIGGVYKISSWADITNAHGVTGNGVVEGLKQGAKETTTNQEPRGLLMLAELSSV
GSLAYGEYSQKTVEIAKSDKEFVIGFIAQRDMGGQEEGFDWLIMTPGVGLDDKGDGLG
QQYRTVDEVVSTGTDIIIVGRGLFGKGRDPDIEGKRYRNAGWNAYLKKTGQL"
rep_origin 3608..4288
/note="ARS3"
regulatory complement(4365..4448)
/note="lac"
/regulatory_class="promoter"
rep_origin complement(4540..4942)
/note="pMB1 ori"
CDS complement(5483..6343)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87327.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1827 a 1470 c 1323 g 1923 t
ORIGIN
1 tcgcgcgcga gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg
61 ccgctacgaa tgttagagac aaaatgtagt ccaggaccgg ccagacgcag tgtgcgtgct
121 ggttgtgcaa ttatactagc acatctaggt gttttaggaa gaaacgtcca ccaccaaaaa
181 attaatttcc aaatattggc acttttatta caccagtggt gttacacagc cccccaaaca
241 ctatcccggt tgttagattg aagtttgttt aacaaaaatt ggaagttcta ttttttactt
301 tttgtacaaa atttggcaag aaaattggag taaaatattt ttatataaat ttcaaactaa
361 aaatatccaa aacataaaat aatacacaaa atgagatgct ctatttagcc aaaccaacca
421 ttacgggtgt gttgtttggt gttgtctgac catgggtata ccatttgtta gtgtatagct
481 gcactgtttt atgtttttcg atttttccac ataaaaggag tactcctata ctaataacaa
541 acactccact ataattggca accacaatta tgcctgggga aatattttcc actccgagtg
601 tacatacatt tttggtgtgt tgttggtggt tgaaaattgt gtgggtcacg tgcatttaat
661 tagggttagg gctacggttt gtgtgcagta ccacgttcaa attgaggctc tctggtataa
721 cttgcactac ctcatcgcta ccccggattt ttttttggta tgatcctcta cgccggacgc
781 atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc
841 accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt
901 aaggtggcag gctcggtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc
961 cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag
1021 tcgcataagg gagagcgtcg agcaagacgt ttcccgttga atatggctca taacacccct
1081 tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg
1141 tgcaatgtaa catcagagat tttgagacac aacgtggctt tgttgaataa atcgaacttt
1201 tgctgagttg aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa
1261 gcaaaagttc aaaatcacca actggtccac ctacaacaaa gctctcatca accgtggctc
1321 cctcactttc tggctggatg atggggcgat tcaggcctgg tatgagtcag caacaccttc
1381 ttcacgaggc agacctcagc gctattctga ccttgccatc acgactgtgc tggtcattaa
1441 acgcgtattc aggctgaccc tgcgcgctgc gcagggcttt attgattcca tttttacact
1501 gatgaatgtt ccgttgcgct gcccggatta cagctagaag gaccaccttt gattgtaaat
1561 agtaataatt accaccctta tttaattatt tatttaactt atttatttat ttattctaca
1621 tatatacaaa tctaataaag tgaaaatctc ccccttcaca tttataattg gccagtcttt
1681 ttcaaataag cattccaacc agcatttcta taccttttac cttcaatatc tggatctctt
1741 cctttaccaa acaatcctct accaacaatg ataatatcag ttccagtgct aacaacttca
1801 tcaacagttc tatattgttg tcctaatcca tcacctttat catctaatcc aactccaggt
1861 gtcataataa gccaatcaaa tccttcttct tggccaccca tatcacgttg ggcaataaat
1921 ccaataacaa attccttatc ggatttagca atttcaacag ttttttgaga atattctcca
1981 tatgctaatg atcccactga tgataattca gctaacatca ataaccctct tggctcttgg
2041 ttggtggtgg tttctttagc tccctgtttt aatccttcaa ccactccatt cccagtgaca
2101 ccatgagcat tggtaatatc tgcccaacta ctaattttat aaactccacc aatatattgt
2161 ttctttacgg tattaccaat atcagcaaat tttctatctt caaaaatcat aaattgatgt
2221 ttacgtgaaa gttctaataa tggttcaata gtggattcat aggaaaaatc attgattata
2281 tcaatatgag tcttgattaa gcatacataa ggacctaatt tatcaattaa ttcaaggaat
2341 tccttagtgg tatcaacgtc aattgatgca cataaattgg ttttcttcag ttccattaat
2401 cgaaataatc gctgtgctac tggtgaggca tgagtttctg ctctctcact ataggtctta
2461 gtgttgactg tcatatcttt tgtttgctat ttctatttct atttctttct tcaactgtaa
2521 aaggggcggt atataaacca gtgtgtatgg ggttgttgct caggaattga tcaatgttta
2581 aaagaatggt tgaaatcatc attgaagaac aaaaaaaaag attttttctt tcattatgtt
2641 atctgtataa tatgcaggaa ataagattgc aaaaaaatag ccgttttttt ggatactatc
2701 aaacaagagg aatgactaat acagaattag tgttacgaat caatggcact acagcaactt
2761 tcaacaaaat aatctacgtt caaaatataa tatatactat taaataaaat caaataataa
2821 aataatttta aagaaagtag taccagctct tttttttgtt tccgtttata ccatccaaat
2881 caattcctat tagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
2941 ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct
3001 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
3061 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
3121 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
3181 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
3241 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
3301 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
3361 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaat tctcatgttt
3421 gacagcttat catcgcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
3481 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
3541 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattc
3601 ccggggatcc agacttgtta aatctgatgt tttgacgatt atcattgaat gtaagatcaa
3661 taaactattc tctttctttt tatttcaaaa ccttgcagta ccatggaatt atatcaagcc
3721 atgttttaaa ttgatcagta gtctaatcgg gcttcaaggg aaaaggtggt ttactcgctt
3781 aacgatactt ttgacatttc tactacttag attgtaagag agcattggta gcatcaaaaa
3841 cacacaaaat ataatcattt tcccaattgg gaacaattca aaataaaaac aactttgatg
3901 cgaagaactt caatatttta gctaattata tcaaaattgg aatggagtct ctactactac
3961 atgcttttct caatgaaatt acgttaaaac ttttctcgtc ttcttccatt tttttttata
4021 ttctttactt tttctttaat ttttttttgt cattccttat ttaaatttcc agaataccct
4081 ttaaactaca taatatcatg aagaatccat ttgacagtgg cagtgacgat gaagatccat
4141 ttcttagtaa tccacaatct gcaccatcaa tgccctacgc agcatatact agtgacagaa
4201 catcgccccg caagacatac caaccattga attttgacag tgaggacgaa gatgctaaag
4261 aaagcgaatt tatggctttc ccactgtcga cctgcaggca tgcaagcttg gcgtaatcat
4321 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
4381 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
4441 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
4501 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
4561 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
4621 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
4681 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
4741 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
4801 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
4861 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
4921 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
4981 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
5041 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
5101 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
5161 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
5221 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
5281 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
5341 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
5401 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
5461 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
5521 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
5581 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
5641 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
5701 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
5761 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
5821 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
5881 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
5941 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
6001 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
6061 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
6121 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
6181 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
6241 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
6301 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
6361 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
6421 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
6481 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
6541 gtc
//