LOCUS LT727061 6575 bp DNA circular SYN 06-FEB-2017 DEFINITION Shuttle vector pMSK1-E, complete sequence. ACCESSION LT727061 VERSION LT727061.1 KEYWORDS . SOURCE Shuttle vector pMSK1-E ORGANISM Shuttle vector pMSK1-E other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 6575) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..6575 /organism="Shuttle vector pMSK1-E" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 4981." /db_xref="taxon:1945225" rep_origin complement(36..111) /note="SV40 ori" rep_origin complement(262..374) /note="polyoma ori" regulatory complement(1066..1162) /note="SV40 polyA early" /regulatory_class="terminator" regulatory 1066..1162 /note="SV40 polyA late" /regulatory_class="terminator" misc_feature complement(1163..1561) /note="3' UTR of SV40 small t-antigen" misc_feature complement(1562..1628) /note="intron of SV40 small t-antigen" CDS complement(1629..>1703) /codon_start=1 /transl_table=11 /note="part of SV40 small t-antigen" /protein_id="SJL87319.1" /translation="DLCEGTLLLWCDIIGQTTYRDLKL" regulatory 1711..1727 /note="SP6 promoter" /regulatory_class="promoter" misc_feature complement(1871..1909) /note="E-tag" CDS complement(<1919..4324) /transl_table=11 /note="hMSK1" /protein_id="SJL87320.1" /translation="MEEEGGSSGGAAGTSADGGDGGEQLLTVKHELRTANLTGHAEKV GIENFELLKVLGTGAYGKVFLVRKISGHDTGKLYAMKVLKKATIVQKAKTTEHTRTER QVLEHIRQSPFLVTLHYAFQTETKLHLILDYINGGELFTHLSQRERFTEHEVQIYVGE IVLALEHLHKLGIIYRDIKLENILLDSNGHVVLTDFGLSKEFVADETERAYSFCGTIE YMAPDIVRGGDSGHDKAVDWWSLGVLMYELLTGASPFTVDGEKNSQAEISRRILKSEP PYPQEMSALAKDLIQRLLMKDPKKRLGCGPRDADEIKEHLFFQKINWDDLAAKKVPAP FKPVIRDELDVSNFAEEFTEMDPTYSPAALPQSSEKLFQGYSFVAPSILFKRNAAVID PLQFHMGVERPGVTNVARSAMMKDSPFYQHYDLDLKDKPLGEGSFSICRKCVHKKSNQ AFAVKIISKRMEANTQKEITALKLCEGHPNIVKLHEVFHDQLHTFLVMELLNGGELFE RIKKKKHFSETEASYIMRKLVSAVSHMHDVGVVHRDLKPENLLFTDENDNLEIKIIDF GFARLKPPDNQPLKTPCFTLHYAAPELLNQNGYDESCDLWSLGVILYTMLSGQVPFQS HDRSLTCTSAVEIMKKIKKGDFSFEGEAWKNVSQEAKDLIQGLLTVDPNKRLKMSGLR YNEWLQDGSQLSSNPLMTPDILGSSGAAVHTCVKATFHAFNKYKREGFCLQNVDKAPL AKRRKMKKTSTSTETRSSSSESSHSSSSHSHGKTTPTKTLQPSNPADSNNPETLFQFS DSVA" regulatory complement(4390..4406) /note="T7 promoter" /regulatory_class="promoter" regulatory complement(4451..5034) /note="hCMV-IE promoter and enhancer" /regulatory_class="promoter" misc_feature complement(5189..5391) /note="supF" rep_origin 5515..5973 /note="M13 ori" rep_origin complement(5992..6224) /note="pMB1 ori" BASE COUNT 1668 a 1566 c 1493 g 1848 t ORIGIN 1 gaattagctt tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata 61 gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg 121 gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagta agacccgcac 181 ccgttcccaa actgcttatt aaagggggta tggaggtgct ggaccttgtg acagggccag 241 acagtgtgac agaaatagaa gcagtcctgt tttgacaagt tgcctctgga agcctctaca 301 atgcctctct tctttttctc cagagtaagc gggcccaggg gtccccggcc tctgcttaat 361 actaaaaaaa acagctgttg tcatagtaat gattgggtgg aaacattcta ggcctgggtg 421 gagaggcttt tgcttcctct tgcaaaacca cacgccctct ggagggcgtt gcctagcaac 481 taattaaaag aggatgtcgc acggccagct gcgtcagtta gtccacttcc tgcttaactg 541 acttgacatt ttctatttta agagtcggga ggaaaattac tgtgttggag gccctccgcc 601 atcttctgaa gctgatcaag taagtgaatt ttcaaaatgg gagccgcact gactattcta 661 gtagacctca tcgagggatt agctgaagtg tctaccctta cgggactctc ggcagaagct 721 attttatctg gagaagccct cgctgccctt gatggcgaaa ttacagctct gactttggag 781 ggtgtaatga gttcggagac agccctagca actatgggta tttcagagga ggtgtatggg 841 tttgtcagta ctgtgcctgt gtttgtaagt cgaacagcgg gggctatatg gctgatgcag 901 acagttcaag gtgcctctac tatttcccta ggaatacagc ggtacctaca caacgaagag 961 gtccctactg taaatagaaa tatggcgttg ataccatggc gggatgatcc agacatgata 1021 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt 1081 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt 1141 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt 1201 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatctcta gtcaaggcac 1261 tatacatcaa atatttttcc ataattttct tgtatagcag tgcagctttt tcctttgtgg 1321 tgtaaatagc aaagcaagca agagttctat tactaaacac agcatgactc aaaaaactta 1381 gcaattctga aggaaagtcc ttggggtctt ctacctttct cttctttttt ggaggagtag 1441 aatgttgaga gtcagcagta gcctcatcat cactagatgg catttcttct gagcaaaaca 1501 ggttttcctc attaaaggca ttccaccact gctcccattc atcagttcca taggttggaa 1561 tctaaaatac acaaacaatt agaatcagta gtttaacaca ttatacactt aaaaatttta 1621 tatttacctt agagctttaa atctctgtag gtagtttgtc caattatgtc acaccacaga 1681 agtaaggttc cttcacaaag atcctctagc atttaggtga cactatagaa tagggccctc 1741 tagagtcgac attccacaga cagccctcat agttagcgta acgatctaaa gttttgtcgt 1801 ctttccagac gttagtaaat gaattttctg tatgaggttt tgctaaacaa ctttcaacag 1861 tctatgcggc acgcggttcc agcggatccg gatacggcac cggcgcacct gcggccgcag 1921 ctactgagtc cgagaactgg aagagggtct ccgggttatt gctgtcggca ggattgctgg 1981 gctgcagtgt cttggtgggt gtagttttac cgtgagaatg agaggaagaa gaatgggaac 2041 tctcactgga actgctgcgc gtctcggtac tggtgctagt ctttttcatt tttcttctct 2101 tagccaaagg ggccttatca acattctgaa ggcaaaaccc ctctctcttg tatttgttaa 2161 aggcgtggaa ggttgctttc acacaggtat gcacggcagc tccggaagat cctagaatat 2221 ccggagtcat cagaggattg gaggacagct gacttccatc ttgtagccat tcattgtacc 2281 tcaagccaga cattttaagc cttttgtttg gatctactgt gagaagtcct tggatcaaat 2341 ctttagcctc ttgggataca ttcttccagg cttctccttc aaaggagaaa tctccctttt 2401 taattttctt catgatttcc accgcgctgg tacacgtcaa acttcggtca tgagattgga 2461 agggaacctg tcctgacaac attgtgtaca aaatgacgcc caagctccac aggtcacagg 2521 actcatcgta gccgttctga ttcaagagct ctggggcggc ataatgaagg gtgaagcatg 2581 gagtcttcag gggctgatta tccggtggct ttagccgtgc aaatccaaaa tcaattattt 2641 taatttccaa attgtcattt tcatcggtga acaataaatt ctcaggtttc agatccctgt 2701 gcaccactcc aacatcatgc atgtggctta cagctgaaac aagcttcctc atgatgtagc 2761 tggcttccgt ctcactgaag tgcttctttt tcttaatgcg ctcaaacagt tctcctccat 2821 tcagaagttc catcactaga aacgtgtgaa gctgatcatg aaaaacttca tgcaacttca 2881 caatattggg gtgtccttca cagagtttca gagctgttat ttccttttga gtattggctt 2941 ccatcctttt gctgattatt ttgactgcaa aagcttggtt acttttttta tgcacacact 3001 ttcgacaaat tgaaaaacta ccttctccca ggggtttgtc cttcaaatct aggtcatagt 3061 gttgatagaa tggagagtcc ttcatcattg cactcctggc aacatttgtc actccaggac 3121 gttcaactcc catgtgaaac tgaagagggt ctatgacagc tgcattacgc ttgaatagga 3181 tggaaggagc aacaaaggaa tagccctgaa acagcttctc agaactctgg ggcagggctg 3241 cgggagaata agtgggatcc atttctgtga actcttctgc aaagttactc acatctaatt 3301 catctcgaat gactggctta aatggtgcag gcactttttt ggcggctaaa tcatcccaat 3361 ttattttctg aaagaagaga tgttctttga tttcatctgc atcacgtgga ccacatccca 3421 atctcttctt gggatctttc atcaaaagac gctgaattag gtctttcgct aaagcactca 3481 tttcttgggg atatggaggc tcacttttta atattctcct agatatctca gcttgggaat 3541 ttttttctcc atcaacagtg aaaggagatg ctccagttag taattcatac attagaacac 3601 ccaaactcca ccagtcaact gccttgtcat gtcctgaatc tccccctctg acaatatctg 3661 gtgccatgta ttcaatagtt ccacaaaagg aatatgctct ttcagtttca tcagccacaa 3721 actccttact cagaccaaaa tctgtcagca ccacatggcc attagaatca agtagaatat 3781 tctcaagctt aatatcacga tatataatcc ccaacttgtg gagatgttcg agggcaagca 3841 caatctctcc aacataaatc tgcacctcat gctctgtgaa acgctctctt tgagaaagat 3901 gagtaaaaag ttcaccacca tttatataat ctaaaatgag atgaagtttg gtttctgtct 3961 ggaaagcata atgtaatgtt accaaaaatg gcgactgcct aatgtgttcc aggacttgtc 4021 gttctgtcct tgtatgctct gtggttttgg ccttttgaac gattgttgcc tttttcaaaa 4081 ctttcatggc atacagcttt ccagtatcat ggccacttat tttacgaact agaaatactt 4141 ttccataagc tccagttcct aggaccttca ggagctcaaa attttctatt cccaccttct 4201 cagcatgtcc tgtcaaatta gcagtccgca gctcgtgctt gacagtgagg agctgctctc 4261 ctccgtcgcc gccgtccgcg ctggtccccg cggcgccgcc gctgctgcca ccctcctcct 4321 ccatggaatt ccagcacact ggcggccgtt actagtggat ccgagctcgg taccaagctt 4381 gggtctccct atagtgagtc gtattaattt cgataagcca gtaagcagtg ggttctctag 4441 ttagccagag agctctgctt atatagacct cccaccgtac acgcctaccg cccatttgcg 4501 tcaatggggc ggagttgtta cgacattttg gaaagtcccg ttgattttgg tgccaaaaca 4561 aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca aaccgctatc 4621 cacgcccatt gatgtactgc caaaaccgca tcaccatggt aatagcgatg actaatacgt 4681 agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa tgccaggcgg 4741 gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac acttgatgta 4801 ctgccaagtg ggcagtttac cgtaaatagt ccacccattg acgtcaatgg aaagtcccta 4861 ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg 4921 gtcagccagg cgggccattt accgtaagtt atgtaacgcg gaactccata tatgggctat 4981 gaactaatga ccccgtaatt gattactatt aataactagt caataatcaa tgtcaacgcg 5041 tatatctggc ccgtacatcg cgaagcagcg caaaacgcct aaccctaagc agattcttca 5101 tgcaattgtc ggtcaagcct tgccttgttg tagcttaaat tttactcgcg cactactcag 5161 cgacctccaa cacacaagca gggagcagat tctttcggac ttttgaaagt gatggtggtg 5221 ggggaaggat tcgaaccttc gaagtcgatg acggcagatt tagagtctgc tccctttggc 5281 cgctcgggaa ccccaccacg ggtaatgctt ttactggcct gctcccttat cgggaagcgg 5341 ggcgcatcat atcaaatgac gcgccgctgt aaagtgttac gttgagaaag accgcggtaa 5401 tccagctggc gtaccgttcc tgtctaaaat ccctttaatc ggcctcctgt ttagctcccg 5461 ctctgattcc aacgaggaaa gcacgttata cggtctcgtc aaagcaacca tagtacgcgc 5521 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 5581 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 5641 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt 5701 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggcggccat 5761 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac 5821 tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt gatttataag 5881 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 5941 cgaattttaa caaaatatta acgtttacaa tttctagcta gaagctagct tgcgttgctg 6001 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 6061 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 6121 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 6181 ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 6241 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 6301 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 6361 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 6421 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca 6481 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 6541 ggtggttttt ttgtttgcaa gcagcagatt acgcc //