LOCUS       LT727061                6575 bp    DNA     circular SYN 06-FEB-2017
DEFINITION  Shuttle vector pMSK1-E, complete sequence.
ACCESSION   LT727061
VERSION     LT727061.1
KEYWORDS    .
SOURCE      Shuttle vector pMSK1-E
  ORGANISM  Shuttle vector pMSK1-E
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 6575)
  AUTHORS   De Schamphelaire W.
  JOURNAL   Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM.
REFERENCE   2
  AUTHORS   De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
            Roggeman Fonseca M., Vanhoucke M., Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished.
FEATURES             Location/Qualifiers
     source          1..6575
                     /organism="Shuttle vector pMSK1-E"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent
                     University,Belgium) accession number LMBP 4981."
                     /db_xref="taxon:1945225"
     rep_origin      complement(36..111)
                     /note="SV40 ori"
     rep_origin      complement(262..374)
                     /note="polyoma ori"
     regulatory      complement(1066..1162)
                     /note="SV40 polyA early"
                     /regulatory_class="terminator"
     regulatory      1066..1162
                     /note="SV40 polyA late"
                     /regulatory_class="terminator"
     misc_feature    complement(1163..1561)
                     /note="3' UTR of SV40 small t-antigen"
     misc_feature    complement(1562..1628)
                     /note="intron of SV40 small t-antigen"
     CDS             complement(1629..>1703)
                     /codon_start=1
                     /transl_table=11
                     /note="part of SV40 small t-antigen"
                     /protein_id="SJL87319.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     regulatory      1711..1727
                     /note="SP6 promoter"
                     /regulatory_class="promoter"
     misc_feature    complement(1871..1909)
                     /note="E-tag"
     CDS             complement(<1919..4324)
                     /transl_table=11
                     /note="hMSK1"
                     /protein_id="SJL87320.1"
                     /translation="MEEEGGSSGGAAGTSADGGDGGEQLLTVKHELRTANLTGHAEKV
                     GIENFELLKVLGTGAYGKVFLVRKISGHDTGKLYAMKVLKKATIVQKAKTTEHTRTER
                     QVLEHIRQSPFLVTLHYAFQTETKLHLILDYINGGELFTHLSQRERFTEHEVQIYVGE
                     IVLALEHLHKLGIIYRDIKLENILLDSNGHVVLTDFGLSKEFVADETERAYSFCGTIE
                     YMAPDIVRGGDSGHDKAVDWWSLGVLMYELLTGASPFTVDGEKNSQAEISRRILKSEP
                     PYPQEMSALAKDLIQRLLMKDPKKRLGCGPRDADEIKEHLFFQKINWDDLAAKKVPAP
                     FKPVIRDELDVSNFAEEFTEMDPTYSPAALPQSSEKLFQGYSFVAPSILFKRNAAVID
                     PLQFHMGVERPGVTNVARSAMMKDSPFYQHYDLDLKDKPLGEGSFSICRKCVHKKSNQ
                     AFAVKIISKRMEANTQKEITALKLCEGHPNIVKLHEVFHDQLHTFLVMELLNGGELFE
                     RIKKKKHFSETEASYIMRKLVSAVSHMHDVGVVHRDLKPENLLFTDENDNLEIKIIDF
                     GFARLKPPDNQPLKTPCFTLHYAAPELLNQNGYDESCDLWSLGVILYTMLSGQVPFQS
                     HDRSLTCTSAVEIMKKIKKGDFSFEGEAWKNVSQEAKDLIQGLLTVDPNKRLKMSGLR
                     YNEWLQDGSQLSSNPLMTPDILGSSGAAVHTCVKATFHAFNKYKREGFCLQNVDKAPL
                     AKRRKMKKTSTSTETRSSSSESSHSSSSHSHGKTTPTKTLQPSNPADSNNPETLFQFS
                     DSVA"
     regulatory      complement(4390..4406)
                     /note="T7 promoter"
                     /regulatory_class="promoter"
     regulatory      complement(4451..5034)
                     /note="hCMV-IE promoter and enhancer"
                     /regulatory_class="promoter"
     misc_feature    complement(5189..5391)
                     /note="supF"
     rep_origin      5515..5973
                     /note="M13 ori"
     rep_origin      complement(5992..6224)
                     /note="pMB1 ori"
BASE COUNT         1668 a         1566 c         1493 g         1848 t
ORIGIN      
        1 gaattagctt tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata
       61 gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg
      121 gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagta agacccgcac
      181 ccgttcccaa actgcttatt aaagggggta tggaggtgct ggaccttgtg acagggccag
      241 acagtgtgac agaaatagaa gcagtcctgt tttgacaagt tgcctctgga agcctctaca
      301 atgcctctct tctttttctc cagagtaagc gggcccaggg gtccccggcc tctgcttaat
      361 actaaaaaaa acagctgttg tcatagtaat gattgggtgg aaacattcta ggcctgggtg
      421 gagaggcttt tgcttcctct tgcaaaacca cacgccctct ggagggcgtt gcctagcaac
      481 taattaaaag aggatgtcgc acggccagct gcgtcagtta gtccacttcc tgcttaactg
      541 acttgacatt ttctatttta agagtcggga ggaaaattac tgtgttggag gccctccgcc
      601 atcttctgaa gctgatcaag taagtgaatt ttcaaaatgg gagccgcact gactattcta
      661 gtagacctca tcgagggatt agctgaagtg tctaccctta cgggactctc ggcagaagct
      721 attttatctg gagaagccct cgctgccctt gatggcgaaa ttacagctct gactttggag
      781 ggtgtaatga gttcggagac agccctagca actatgggta tttcagagga ggtgtatggg
      841 tttgtcagta ctgtgcctgt gtttgtaagt cgaacagcgg gggctatatg gctgatgcag
      901 acagttcaag gtgcctctac tatttcccta ggaatacagc ggtacctaca caacgaagag
      961 gtccctactg taaatagaaa tatggcgttg ataccatggc gggatgatcc agacatgata
     1021 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     1081 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     1141 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     1201 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatctcta gtcaaggcac
     1261 tatacatcaa atatttttcc ataattttct tgtatagcag tgcagctttt tcctttgtgg
     1321 tgtaaatagc aaagcaagca agagttctat tactaaacac agcatgactc aaaaaactta
     1381 gcaattctga aggaaagtcc ttggggtctt ctacctttct cttctttttt ggaggagtag
     1441 aatgttgaga gtcagcagta gcctcatcat cactagatgg catttcttct gagcaaaaca
     1501 ggttttcctc attaaaggca ttccaccact gctcccattc atcagttcca taggttggaa
     1561 tctaaaatac acaaacaatt agaatcagta gtttaacaca ttatacactt aaaaatttta
     1621 tatttacctt agagctttaa atctctgtag gtagtttgtc caattatgtc acaccacaga
     1681 agtaaggttc cttcacaaag atcctctagc atttaggtga cactatagaa tagggccctc
     1741 tagagtcgac attccacaga cagccctcat agttagcgta acgatctaaa gttttgtcgt
     1801 ctttccagac gttagtaaat gaattttctg tatgaggttt tgctaaacaa ctttcaacag
     1861 tctatgcggc acgcggttcc agcggatccg gatacggcac cggcgcacct gcggccgcag
     1921 ctactgagtc cgagaactgg aagagggtct ccgggttatt gctgtcggca ggattgctgg
     1981 gctgcagtgt cttggtgggt gtagttttac cgtgagaatg agaggaagaa gaatgggaac
     2041 tctcactgga actgctgcgc gtctcggtac tggtgctagt ctttttcatt tttcttctct
     2101 tagccaaagg ggccttatca acattctgaa ggcaaaaccc ctctctcttg tatttgttaa
     2161 aggcgtggaa ggttgctttc acacaggtat gcacggcagc tccggaagat cctagaatat
     2221 ccggagtcat cagaggattg gaggacagct gacttccatc ttgtagccat tcattgtacc
     2281 tcaagccaga cattttaagc cttttgtttg gatctactgt gagaagtcct tggatcaaat
     2341 ctttagcctc ttgggataca ttcttccagg cttctccttc aaaggagaaa tctccctttt
     2401 taattttctt catgatttcc accgcgctgg tacacgtcaa acttcggtca tgagattgga
     2461 agggaacctg tcctgacaac attgtgtaca aaatgacgcc caagctccac aggtcacagg
     2521 actcatcgta gccgttctga ttcaagagct ctggggcggc ataatgaagg gtgaagcatg
     2581 gagtcttcag gggctgatta tccggtggct ttagccgtgc aaatccaaaa tcaattattt
     2641 taatttccaa attgtcattt tcatcggtga acaataaatt ctcaggtttc agatccctgt
     2701 gcaccactcc aacatcatgc atgtggctta cagctgaaac aagcttcctc atgatgtagc
     2761 tggcttccgt ctcactgaag tgcttctttt tcttaatgcg ctcaaacagt tctcctccat
     2821 tcagaagttc catcactaga aacgtgtgaa gctgatcatg aaaaacttca tgcaacttca
     2881 caatattggg gtgtccttca cagagtttca gagctgttat ttccttttga gtattggctt
     2941 ccatcctttt gctgattatt ttgactgcaa aagcttggtt acttttttta tgcacacact
     3001 ttcgacaaat tgaaaaacta ccttctccca ggggtttgtc cttcaaatct aggtcatagt
     3061 gttgatagaa tggagagtcc ttcatcattg cactcctggc aacatttgtc actccaggac
     3121 gttcaactcc catgtgaaac tgaagagggt ctatgacagc tgcattacgc ttgaatagga
     3181 tggaaggagc aacaaaggaa tagccctgaa acagcttctc agaactctgg ggcagggctg
     3241 cgggagaata agtgggatcc atttctgtga actcttctgc aaagttactc acatctaatt
     3301 catctcgaat gactggctta aatggtgcag gcactttttt ggcggctaaa tcatcccaat
     3361 ttattttctg aaagaagaga tgttctttga tttcatctgc atcacgtgga ccacatccca
     3421 atctcttctt gggatctttc atcaaaagac gctgaattag gtctttcgct aaagcactca
     3481 tttcttgggg atatggaggc tcacttttta atattctcct agatatctca gcttgggaat
     3541 ttttttctcc atcaacagtg aaaggagatg ctccagttag taattcatac attagaacac
     3601 ccaaactcca ccagtcaact gccttgtcat gtcctgaatc tccccctctg acaatatctg
     3661 gtgccatgta ttcaatagtt ccacaaaagg aatatgctct ttcagtttca tcagccacaa
     3721 actccttact cagaccaaaa tctgtcagca ccacatggcc attagaatca agtagaatat
     3781 tctcaagctt aatatcacga tatataatcc ccaacttgtg gagatgttcg agggcaagca
     3841 caatctctcc aacataaatc tgcacctcat gctctgtgaa acgctctctt tgagaaagat
     3901 gagtaaaaag ttcaccacca tttatataat ctaaaatgag atgaagtttg gtttctgtct
     3961 ggaaagcata atgtaatgtt accaaaaatg gcgactgcct aatgtgttcc aggacttgtc
     4021 gttctgtcct tgtatgctct gtggttttgg ccttttgaac gattgttgcc tttttcaaaa
     4081 ctttcatggc atacagcttt ccagtatcat ggccacttat tttacgaact agaaatactt
     4141 ttccataagc tccagttcct aggaccttca ggagctcaaa attttctatt cccaccttct
     4201 cagcatgtcc tgtcaaatta gcagtccgca gctcgtgctt gacagtgagg agctgctctc
     4261 ctccgtcgcc gccgtccgcg ctggtccccg cggcgccgcc gctgctgcca ccctcctcct
     4321 ccatggaatt ccagcacact ggcggccgtt actagtggat ccgagctcgg taccaagctt
     4381 gggtctccct atagtgagtc gtattaattt cgataagcca gtaagcagtg ggttctctag
     4441 ttagccagag agctctgctt atatagacct cccaccgtac acgcctaccg cccatttgcg
     4501 tcaatggggc ggagttgtta cgacattttg gaaagtcccg ttgattttgg tgccaaaaca
     4561 aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca aaccgctatc
     4621 cacgcccatt gatgtactgc caaaaccgca tcaccatggt aatagcgatg actaatacgt
     4681 agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa tgccaggcgg
     4741 gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac acttgatgta
     4801 ctgccaagtg ggcagtttac cgtaaatagt ccacccattg acgtcaatgg aaagtcccta
     4861 ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg
     4921 gtcagccagg cgggccattt accgtaagtt atgtaacgcg gaactccata tatgggctat
     4981 gaactaatga ccccgtaatt gattactatt aataactagt caataatcaa tgtcaacgcg
     5041 tatatctggc ccgtacatcg cgaagcagcg caaaacgcct aaccctaagc agattcttca
     5101 tgcaattgtc ggtcaagcct tgccttgttg tagcttaaat tttactcgcg cactactcag
     5161 cgacctccaa cacacaagca gggagcagat tctttcggac ttttgaaagt gatggtggtg
     5221 ggggaaggat tcgaaccttc gaagtcgatg acggcagatt tagagtctgc tccctttggc
     5281 cgctcgggaa ccccaccacg ggtaatgctt ttactggcct gctcccttat cgggaagcgg
     5341 ggcgcatcat atcaaatgac gcgccgctgt aaagtgttac gttgagaaag accgcggtaa
     5401 tccagctggc gtaccgttcc tgtctaaaat ccctttaatc ggcctcctgt ttagctcccg
     5461 ctctgattcc aacgaggaaa gcacgttata cggtctcgtc aaagcaacca tagtacgcgc
     5521 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     5581 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     5641 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     5701 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggcggccat
     5761 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     5821 tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt gatttataag
     5881 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     5941 cgaattttaa caaaatatta acgtttacaa tttctagcta gaagctagct tgcgttgctg
     6001 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     6061 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     6121 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     6181 ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     6241 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     6301 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     6361 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     6421 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     6481 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     6541 ggtggttttt ttgtttgcaa gcagcagatt acgcc
//