LOCUS LT727060 6218 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-EmCASP-8-C362A, complete
sequence.
ACCESSION LT727060
VERSION LT727060.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-EmCASP-8-C362A
ORGANISM Mammalian expression vector pCAGGS-EmCASP-8-C362A
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6218)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6218
/organism="Mammalian expression vector
pCAGGS-EmCASP-8-C362A"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4942."
/db_xref="taxon:1944811"
misc_feature 16..54
/note="E-tag"
CDS 64..1506
/transl_table=11
/note="mouse CASP-8-C362A"
/protein_id="SJL87317.1"
/translation="MDFQSCLYAIAEELGSEDLAALKFLCLDYIPHKKQETIEDAQKL
FLRLREKGMLEEGNLSFLKELLFHISRWDLLVNFLDCNREEMVRELRDPDNVQISPYR
VMLFKLSEEVSELELRSFKFLLNNEIPKCKLEDDLSLLEIFVEMEKRTMLAENNLETL
KSICDQVNKSLLGKIEDYERSSTERRMSLEGREELPPSVLDEMSLKMAELCDSPREQD
SESRTSDKVYQMKNKPRGYCLIINNHDFSKAREDITQLRKMKDRKGTDCDKEALSKTF
KELHFEIVSYDDCTANEIHEILEGYQSADHKNKDCFICCILSHGDKGVVYGTDGKEAS
IYDLTSYFTGSKCPSLSGKPKIFFIQAAQGSNFQKGVPDEAGFEQQNHTLEVDSSSHK
NYIPDEADFLLGMATVKNCVSYRDPVNGTWYIQSLCQSLRERCPQGDDILSILTGVNY
DVSNKDDRRNKGKQMPQPTFTLRKKLFFPP"
regulatory 1508..1599
/note="3' UTR of rabbit beta-globin, incl. polyA"
/regulatory_class="terminator"
misc_feature 1600..1956
/note="3' flanking region rabbit beta-globin"
regulatory complement(2034..2116)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 2258..2333
/note="SV40 ori"
regulatory 2364..2438
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(2364..2438)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(2553..2954)
/note="pMB1 ori"
CDS complement(3495..4355)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87318.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4491..4868
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 4874..6215
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 5243..6167
/note="intron AG promoter"
BASE COUNT 1431 a 1583 c 1700 g 1504 t
ORIGIN
1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
61 gccatggatt tccagagttg tctttatgct attgctgaag aactgggcag tgaagacctg
121 gctgccctca agttcctgtg cttggactac atcccacaca agaagcagga gaccatcgag
181 gatgcccaga agctatttct gaggcttcgg gaaaagggga tgttggagga aggcaatctg
241 tctttcctga aagagctgct tttccacatc agtcggtggg acctgctggt caacttccta
301 gactgcaacc gagaggagat ggtgagagag ctgcgggacc cagacaatgt ccagatttct
361 ccctacaggg tcatgctctt taagctctca gaagaagtga gcgagttgga attgagatct
421 tttaagttcc ttttgaacaa tgagatcccc aaatgtaagc tggaagatga cttgagcctg
481 cttgaaattt ttgtagaaat ggagaagagg accatgctgg cagaaaataa cttggaaacc
541 ctaaaatcaa tctgtgacca ggtcaacaag agcctgctgg ggaagatcga ggattatgaa
601 agatcaagca cagagagaag aatgagtctt gaaggaaggg aagagttgcc accttcagtt
661 ttggatgaga tgagcctcaa aatggcggaa ctgtgtgact cgccaagaga acaagacagt
721 gagtcacgga cttcagacaa agtttaccaa atgaagaaca aacctcgggg atactgtctg
781 atcatcaaca atcatgattt cagcaaggcc cgggaagaca taacccaact ccgaaaaatg
841 aaggacagaa aaggaacaga ctgtgataaa gaggctctga gtaagacctt taaggagctt
901 cattttgaga tagtatctta cgacgactgc actgcaaatg aaatccacga gattctagaa
961 ggctaccaaa gcgcagacca caagaacaaa gactgcttca tctgctgtat cttatcccac
1021 ggtgacaagg gtgtcgtcta tggaacggat gggaaggagg cctccatcta tgacctgaca
1081 tcttacttca ctggttcaaa gtgcccttcc ctgtctggga aacccaagat ctttttcatt
1141 caggctgcgc aaggaagtaa cttccagaaa ggagtgcctg atgaggcagg cttcgagcaa
1201 cagaaccaca ctttagaagt ggattcatca tctcacaaga actatattcc ggatgaggca
1261 gactttctgc tgggaatggc tacggtgaag aactgcgttt cctaccgaga tcctgtgaat
1321 ggaacctggt atattcagtc actttgccag agcctgaggg aaagatgtcc tcaaggagat
1381 gacattctta gcatcctgac tggcgtgaac tatgacgtga gcaataaaga cgacaggagg
1441 aacaagggaa agcagatgcc acagcccacc ttcacactac ggaagaagct cttcttccct
1501 ccctaaggat ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca
1561 tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt
1621 gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta
1681 tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca aaggttggct
1741 ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttat tccatagaaa
1801 agccttgact tgaggttaga ttttttttat attttgtttt gtgttatttt tttctttaac
1861 atccctaaaa ttttccttac atgttttact agccagattt ttcctcctct cctgactact
1921 cccagtcata gctgtccctc ttctcttatg gagatccctc gacctgcagc ccaagcttgg
1981 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
2041 acatacgagc cggaagcata aagtgtaaag cctgggtgcc taatgagtga gctaactcac
2101 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagcggat
2161 ccgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
2221 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
2281 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
2341 ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc ttataatggt tacaaataaa
2401 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
2461 tgtccaaact catcaatgta tcttatcatg tctggatccg ctgcattaat gaatcggcca
2521 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
2581 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
2641 gttatccaca gaatcaggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
2701 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
2761 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
2821 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
2881 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
2941 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
3001 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
3061 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
3121 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
3181 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
3241 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
3301 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
3361 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
3421 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
3481 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
3541 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
3601 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
3661 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
3721 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
3781 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
3841 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
3901 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
3961 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
4021 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
4081 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
4141 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
4201 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
4261 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
4321 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
4381 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
4441 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgggtc gacattgatt
4501 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
4561 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
4621 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
4681 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
4741 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
4801 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
4861 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
4921 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
4981 gggggggggg gggccccccc caggcggggc ggggcggggc gaggggcggg gcggggcgag
5041 gcggaaaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc
5101 gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgttgc
5161 gcgctgcctt ccccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg
5221 actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa
5281 ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg
5341 gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg
5401 cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg
5461 cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc
5521 gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg
5581 gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg
5641 agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct
5701 cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg
5761 ggccggggag ggctcggggg aaggggcgcg gcggcccccg gagcgccggc ggctgtcgag
5821 gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc
5881 ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg
5941 ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg
6001 cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg cggggggacg
6061 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct
6121 ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
6181 tgctggttat tgtgctgtct catcattttg gcaaagaa
//