LOCUS LT727056 9447 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian expression vector pACCMVpLpA-E-hRac1-DN, complete sequence. ACCESSION LT727056 VERSION LT727056.1 KEYWORDS . SOURCE Mammalian expression vector pACCMVpLpA-E-hRac1-DN ORGANISM Mammalian expression vector pACCMVpLpA-E-hRac1-DN other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 9447) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..9447 /organism="Mammalian expression vector pACCMVpLpA-E-hRac1-DN" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 4886." /db_xref="taxon:1944747" rep_origin 1..454 /note="Ad5 ori" regulatory 673..1256 /note="hCMV-IE promoter and enhancer" /regulatory_class="promoter" misc_feature 1300..1338 /note="E-tag" CDS <1348..1923 /codon_start=1 /transl_table=11 /note="hRAC1-DN" /protein_id="SJL87307.1" /translation="QAIKCVVVGDGAVGKNCLLISYTTNAFPGEYIPTVFDNYSANVM VDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRH HCPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALT QRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL" misc_feature 1393..1395 /note="T17N" CDS <1997..2071 /codon_start=1 /transl_table=11 /note="part of SV40 small t-antigen" /protein_id="SJL87308.1" /translation="DLCEGTLLLWCDIIGQTTYRDLKL" misc_feature 2072..2138 /note="intron SV40 small t-antigen" regulatory 2276..2372 /note="SV40 polyA early" /regulatory_class="terminator" regulatory complement(2276..2372) /note="SV40 polyA late" /regulatory_class="terminator" misc_feature 2446..5348 /note="Ad5 flanking np3334-6236" rep_origin complement(7429..7831) /note="pMB1 ori" CDS complement(8372..9232) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL87309.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" BASE COUNT 2129 a 2375 c 2573 g 2370 t ORIGIN 1 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 61 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 121 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg 181 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 241 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga 301 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg 361 gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc 421 cgggtcaaag ttggcgtttt attattatag tcagtctagg cggccgcgat ctatacattg 481 aatcaatatt ggcaattagc catattagtc attggttata tagcataaat caatattggc 541 tattggccat tgcatacgtt gtatctatat cataatatgt acatttatat tggctcatgt 601 ccaatatgac cgccatgttg acattgatta ttgactagtt attaatagta atcaattacg 661 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc 721 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc 781 atagtaacgc caatagggac tttccattga cgtcaataat gacgtatgtt cccatagtaa 841 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact 901 tggcagtaca tcaagtgtat catatgccaa gtccgccccc tattgacgtc aatgacggta 961 aatggcccgc ctggcattat gcccagtaca tgaccttacg ggactttcct acttggcagt 1021 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacaccaatg 1081 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg 1141 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaat aaccccgccc 1201 cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt 1261 tagtgaaccg tcagatccgg tcgcgcgaat tccaccatgg gtgcgccggt gccgtatccg 1321 gacccgctgg aaccgcgtgc ggccgctcag gccatcaagt gtgtggtggt gggagacgga 1381 gctgtaggta aaaattgcct actgatcagt tacacaacca atgcatttcc tggagaatat 1441 atccctactg tctttgacaa ttattctgcc aatgttatgg tagatggaaa accggtgaat 1501 ctgggcttat gggatacagc tggacaagaa gattatgaca gattacgccc cctatcctat 1561 ccgcaaacag atgtgttctt aatttgcttt tcccttgtga gtcctgcatc atttgaaaat 1621 gtccgtgcaa agtggtatcc tgaggtgcgg caccactgtc ccaacactcc catcatccta 1681 gtgggaacta aacttgatct tagggatgat aaagacacga tcgagaaact gaaggagaag 1741 aagctgactc ccatcaccta tccgcagggt ctagccatgg ctaaggagat tggtgctgta 1801 aaatacctgg agtgctcggc gctcacacag cgaggcctca agacagtgtt tgacgaagcg 1861 atccgagcag tcctctgccc gcctcccgtg aagaagagga agagaaaatg cctgctgttg 1921 tgactcgagc ccgggacatt aaacgcgtgg gtacccgggg atcctctaga gtcgacctgc 1981 aggcatgcaa gcttgggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg 2041 acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat 2101 gtgttaaact actgattcta attgtttgtg tattttagat tcacagtccc aaggctcatt 2161 tcaggcccct cagtcctcac agtctgttca tgatcataat cagccatacc acatttgtag 2221 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga 2281 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata 2341 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 2401 aactcatcaa tgtatcttat catgtctgga tcgcggccgc ctagaggaag gtgctgaggt 2461 acgatgagac ccgcaccagg tgcagaccct gcgagtgtgg cggtaaacat attaggaacc 2521 agcctgtgat gctggatgtg accgaggagc tgaggcccga tcacttggtg ctggcctgca 2581 cccgcgctga gtttggctct agcgatgaag atacagattg aggtactgaa atgtgtgggc 2641 gtggcttaag ggtgggaaag aatatataag gtgggggtct tatgtagttt tgtatctgtt 2701 ttgcagcagc cgccgccgcc atgagcacca actcgtttga tggaagcatt gtgagctcat 2761 atttgacaac gcgcatgccc ccatgggccg gggtgcgtca gaatgtgatg ggctccagca 2821 ttgatggtcg ccccgtcctg cccgcaaact ctactacctt gacctacgag accgtgtctg 2881 gaacgccgtt ggagactgca gcctccgccg ccgcttcagc cgctgcagcc accgcccgcg 2941 ggattgtgac tgactttgct ttcctgagcc cgcttgcaag cagtgcagct tcccgttcat 3001 ccgcccgcga tgacaagttg acggctcttt tggcacaatt ggattctttg acccgggaac 3061 ttaatgtcgt ttctcagcag ctgttggatc tgcgccagca ggtttctgcc ctgaaggctt 3121 cctcccctcc caatgcggtt taaaacataa ataaaaaacc agactctgtt tggatttgga 3181 tcaagcaagt gtcttgctgt ctttatttag gggttttgcg cgcgcggtag gcccgggacc 3241 agcggtctcg gtcgttgagg gtcctgtgta ttttttccag gacgtggtaa aggtgactct 3301 ggatgttcag atacatgggc ataagcccgt ctctggggtg gaggtagcac cactgcagag 3361 cttcatgctg cggggtggtg ttgtagatga tccagtcgta gcaggagcgc tgggcgtggt 3421 gcctaaaaat gtctttcagt agcaagctga ttgccagggg caggcccttg gtgtaagtgt 3481 ttacaaagcg gttaagctgg gatgggtgca tacgtgggga tatgagatgc atcttggact 3541 gtatttttag gttggctatg ttcccagcca tatccctccg gggattcatg ttgtgcagaa 3601 ccaccagcac agtgtatccg gtgcacttgg gaaatttgtc atgtagctta gaaggaaatg 3661 cgtggaagaa cttggagacg cccttgtgac ctccaagatt ttccatgcat tcgtccataa 3721 tgatggcaat gggcccacgg gcggcggcct gggcgaagat atttctggga tcactaacgt 3781 catagttgtg ttccaggatg agatcgtcat aggccatttt tacaaagcgc gggcggaggg 3841 tgccagactg cggtataatg gttccatccg gcccaggggc gtagttaccc tcacagattt 3901 gcatttccca cgctttgagt tcagatgggg ggatcatgtc tacctgcggg gcgatgaaga 3961 aaacggtttc cggggtaggg gagatcagct gggaagaaag caggttcctg agcagctgcg 4021 acttaccgca gccggtgggc ccgtaaatca cacctattac cgggtgcaac tggtagttaa 4081 gagagctgca gctgccgtca tccctgagca ggggggccac ttcgttaagc atgtccctga 4141 ctcgcatgtt ttccctgacc aaatccgcca gaaggcgctc gccgcccagc gatagcagtt 4201 cttgcaagga agcaaagttt ttcaacggtt tgagaccgtc cgccgtaggc atgcttttga 4261 gcgtttgacc aagcagttcc aggcggtccc acagctcggt cacctgctct acggcatctc 4321 gatccagcat atctcctcgt ttcgcgggtt ggggcggctt tcgctgtacg gcagtagtcg 4381 gtgctcgtcc agacgggcca gggtcatgtc tttccacggg cgcagggtcc tcgtcagcgt 4441 agtctgggtc acggtgaagg ggtgcgctcc gggctgcgcg ctggccaggg tgcgcttgag 4501 gctggtcctg ctggtgctga agcgctgccg gtcttcgccc tgcgcgtcgg ccaggtagca 4561 tttgaccatg gtgtcatagt ccagcccctc cgcggcgtgg cccttggcgc gcagcttgcc 4621 cttggaggag gcgccgcacg aggggcagtg cagacttttg agggcgtaga gcttgggcgc 4681 gagaaatacc gattccgggg agtaggcatc cgcgccgcag gccccgcaga cggtctcgca 4741 ttccacgagc caggtgagct ctggccgttc ggggtcaaaa accaggtttc ccccatgctt 4801 tttgatgcgt ttcttacctc tggtttccat gagccggtgt ccacgctcgg tgacgaaaag 4861 gctgtccgtg tccccgtata cagacttgag aggcctgtcc tcgagcggtg ttccgcggtc 4921 ctcctcgtat agaaactcgg accactctga gacaaaggct cgcgtccagg ccagcacgaa 4981 ggaggctaag tgggaggggt agcggtcgtt gtccactagg gggtccactc gctccagggt 5041 gtgaagacac atgtcgccct cttcggcatc aaggaaggtg attggtttgt aggtgtaggc 5101 cacgtgaccg ggtgttcctg aaggggggct ataaaagggg gtgggggcgc gttcgtcctc 5161 actctcttcc gcatcgctgt ctgcgagggc cagctgttgg ggtgagtact ccctctgaaa 5221 agcgggcatg acttctgcgc taagattgtc agtttccaaa aacgaggagg atttgatatt 5281 cacctggccc gcggtgatgc ctttgagggt ggccgcatcc atctggtcag aaaagacaat 5341 ctttttgttg tcaagctagc ttctatgggt ttaacaatac ctgtgtggaa gcctggaccg 5401 atgtaagggt tcggggctgt gccttttact gctgctggaa gggggtggtg tgtcgcccca 5461 aaagcagggc ttcaattaag aaatgcctct ttgaaaggtg taccttgggt atcctgtctg 5521 agggtaactc cagggtgcgc cacaatgtgg cctccgactg tggttgcttc atgctagtga 5581 aaagcgtggc tgtgattaag cataacatgg tatgtggcaa ctgcgaggac agggcctctc 5641 agatgctgac ctgctcggac ggcaactgtc acctgctgaa gaccattcac gtagccagcc 5701 actctcgcaa ggcctggcca gtgtttgagc ataacatact gacccgctgt tccttgcatt 5761 tgggtaacag gaggggggtg ttcctacctt accaatgcaa tttgagtcac actaagatat 5821 tgcttgagcc cgagagcatg tccaaggtga acctgaacgg ggtgtttgac atgaccatga 5881 agatctgcgt ctccggtctc gccgcttaga tcgctctgtg tagtagttgt agtatatcca 5941 ctctctcaaa gcatccaggc gccccctggc ttcgggttct atgtaaactc cttcatgcgc 6001 cgctgccctg ataacatcca ccaccgcaga ataagccaca cccagccaac ctacacattc 6061 gttctgcgag tcacacacgg gaggagcggg aagagctgga agaaccatgt tttttttttt 6121 attccaaaag attatccaaa acctcaaaat gaagatcctc tacgccggac gcatcgtggc 6181 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg 6241 ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc 6301 aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc 6361 ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa 6421 gggagagcgt cgatcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt 6481 gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg 6541 taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg 6601 cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct 6661 tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg 6721 cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc 6781 ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt 6841 ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg gctcttacca 6901 gcctaacttc gatcactgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca 6961 catggaacgg gttggcatgg attgtaggcg ccgccctata ccttgtctgc ctccccgcgt 7021 tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat ggaagccggc ggcacctcgc 7081 taacggattc accactccaa gaattggagc caatcaattc ttgcggagaa ctgtgaatgc 7141 gcaaaccaac ccttggcaga acatatccat cgcgtccgcc atctccagca gccgcacgcg 7201 gcgcatctcg ggcagcgttg ggtcctggcc acgggtgcgc atgatcgtgc tcctgtcgtt 7261 gaggacccgg ctaggctggc ggggttgcct tactggttag cagaatgaat caccgatacg 7321 cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa caacatgaat 7381 ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc tcttccgctt 7441 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 7501 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 7561 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 7621 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 7681 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 7741 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 7801 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 7861 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 7921 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 7981 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 8041 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 8101 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 8161 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 8221 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 8281 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 8341 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 8401 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 8461 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac 8521 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 8581 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag 8641 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg 8701 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 8761 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 8821 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc 8881 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 8941 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata 9001 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa 9061 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca 9121 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc 9181 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc 9241 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg 9301 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac 9361 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga 9421 ggccctttcg tcttcaagaa ttaattc //