LOCUS LT727055 9447 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pACCMVpLpA-E-hRac1-CA, complete
sequence.
ACCESSION LT727055
VERSION LT727055.1
KEYWORDS .
SOURCE Mammalian expression vector pACCMVpLpA-E-hRac1-CA
ORGANISM Mammalian expression vector pACCMVpLpA-E-hRac1-CA
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 9447)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..9447
/organism="Mammalian expression vector
pACCMVpLpA-E-hRac1-CA"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4885."
/db_xref="taxon:1944746"
rep_origin 1..454
/note="Ad5 ori"
regulatory 673..1256
/note="hCMV-IE promoter and enhancer"
/regulatory_class="promoter"
misc_feature 1300..1338
/note="E-tag"
CDS <1348..1923
/codon_start=1
/transl_table=11
/note="hRAC1-CA"
/protein_id="SJL87304.1"
/translation="QAIKCVVVGDVAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVM
VDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRH
HCPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALT
QRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL"
misc_feature 1378..1380
/note="G12V"
CDS <1997..2071
/codon_start=1
/transl_table=11
/note="part of SV40 small t-antigen"
/protein_id="SJL87305.1"
/translation="DLCEGTLLLWCDIIGQTTYRDLKL"
misc_feature 2072..2138
/note="intron of SV40 small t-antigen"
regulatory 2276..2372
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(2276..2372)
/note="SV40 polyA late"
/regulatory_class="terminator"
misc_feature 2446..5348
/note="Ad5 flanking np3334-6236"
rep_origin complement(7429..7831)
/note="pMB1 ori"
CDS complement(8372..9232)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87306.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 2128 a 2376 c 2572 g 2371 t
ORIGIN
1 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt
61 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt
121 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg
181 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag
241 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga
301 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg
361 gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc
421 cgggtcaaag ttggcgtttt attattatag tcagtctagg cggccgcgat ctatacattg
481 aatcaatatt ggcaattagc catattagtc attggttata tagcataaat caatattggc
541 tattggccat tgcatacgtt gtatctatat cataatatgt acatttatat tggctcatgt
601 ccaatatgac cgccatgttg acattgatta ttgactagtt attaatagta atcaattacg
661 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
721 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
781 atagtaacgc caatagggac tttccattga cgtcaataat gacgtatgtt cccatagtaa
841 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
901 tggcagtaca tcaagtgtat catatgccaa gtccgccccc tattgacgtc aatgacggta
961 aatggcccgc ctggcattat gcccagtaca tgaccttacg ggactttcct acttggcagt
1021 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacaccaatg
1081 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
1141 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaat aaccccgccc
1201 cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt
1261 tagtgaaccg tcagatccgg tcgcgcgaat tccaccatgg gtgcgccggt gccgtatccg
1321 gacccgctgg aaccgcgtgc ggccgctcag gccatcaagt gtgtggtggt gggagacgta
1381 gctgtaggta aaacttgcct actgatcagt tacacaacca atgcatttcc tggagaatat
1441 atccctactg tctttgacaa ttattctgcc aatgttatgg tagatggaaa accggtgaat
1501 ctgggcttat gggatacagc tggacaagaa gattatgaca gattacgccc cctatcctat
1561 ccgcaaacag atgtgttctt aatttgcttt tcccttgtga gtcctgcatc atttgaaaat
1621 gtccgtgcaa agtggtatcc tgaggtgcgg caccactgtc ccaacactcc catcatccta
1681 gtgggaacta aacttgatct tagggatgat aaagacacga tcgagaaact gaaggagaag
1741 aagctgactc ccatcaccta tccgcagggt ctagccatgg ctaaggagat tggtgctgta
1801 aaatacctgg agtgctcggc gctcacacag cgaggcctca agacagtgtt tgacgaagcg
1861 atccgagcag tcctctgccc gcctcccgtg aagaagagga agagaaaatg cctgctgttg
1921 tgactcgagc ccgggacatt aaacgcgtgg gtacccgggg atcctctaga gtcgacctgc
1981 aggcatgcaa gcttgggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg
2041 acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat
2101 gtgttaaact actgattcta attgtttgtg tattttagat tcacagtccc aaggctcatt
2161 tcaggcccct cagtcctcac agtctgttca tgatcataat cagccatacc acatttgtag
2221 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
2281 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
2341 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
2401 aactcatcaa tgtatcttat catgtctgga tcgcggccgc ctagaggaag gtgctgaggt
2461 acgatgagac ccgcaccagg tgcagaccct gcgagtgtgg cggtaaacat attaggaacc
2521 agcctgtgat gctggatgtg accgaggagc tgaggcccga tcacttggtg ctggcctgca
2581 cccgcgctga gtttggctct agcgatgaag atacagattg aggtactgaa atgtgtgggc
2641 gtggcttaag ggtgggaaag aatatataag gtgggggtct tatgtagttt tgtatctgtt
2701 ttgcagcagc cgccgccgcc atgagcacca actcgtttga tggaagcatt gtgagctcat
2761 atttgacaac gcgcatgccc ccatgggccg gggtgcgtca gaatgtgatg ggctccagca
2821 ttgatggtcg ccccgtcctg cccgcaaact ctactacctt gacctacgag accgtgtctg
2881 gaacgccgtt ggagactgca gcctccgccg ccgcttcagc cgctgcagcc accgcccgcg
2941 ggattgtgac tgactttgct ttcctgagcc cgcttgcaag cagtgcagct tcccgttcat
3001 ccgcccgcga tgacaagttg acggctcttt tggcacaatt ggattctttg acccgggaac
3061 ttaatgtcgt ttctcagcag ctgttggatc tgcgccagca ggtttctgcc ctgaaggctt
3121 cctcccctcc caatgcggtt taaaacataa ataaaaaacc agactctgtt tggatttgga
3181 tcaagcaagt gtcttgctgt ctttatttag gggttttgcg cgcgcggtag gcccgggacc
3241 agcggtctcg gtcgttgagg gtcctgtgta ttttttccag gacgtggtaa aggtgactct
3301 ggatgttcag atacatgggc ataagcccgt ctctggggtg gaggtagcac cactgcagag
3361 cttcatgctg cggggtggtg ttgtagatga tccagtcgta gcaggagcgc tgggcgtggt
3421 gcctaaaaat gtctttcagt agcaagctga ttgccagggg caggcccttg gtgtaagtgt
3481 ttacaaagcg gttaagctgg gatgggtgca tacgtgggga tatgagatgc atcttggact
3541 gtatttttag gttggctatg ttcccagcca tatccctccg gggattcatg ttgtgcagaa
3601 ccaccagcac agtgtatccg gtgcacttgg gaaatttgtc atgtagctta gaaggaaatg
3661 cgtggaagaa cttggagacg cccttgtgac ctccaagatt ttccatgcat tcgtccataa
3721 tgatggcaat gggcccacgg gcggcggcct gggcgaagat atttctggga tcactaacgt
3781 catagttgtg ttccaggatg agatcgtcat aggccatttt tacaaagcgc gggcggaggg
3841 tgccagactg cggtataatg gttccatccg gcccaggggc gtagttaccc tcacagattt
3901 gcatttccca cgctttgagt tcagatgggg ggatcatgtc tacctgcggg gcgatgaaga
3961 aaacggtttc cggggtaggg gagatcagct gggaagaaag caggttcctg agcagctgcg
4021 acttaccgca gccggtgggc ccgtaaatca cacctattac cgggtgcaac tggtagttaa
4081 gagagctgca gctgccgtca tccctgagca ggggggccac ttcgttaagc atgtccctga
4141 ctcgcatgtt ttccctgacc aaatccgcca gaaggcgctc gccgcccagc gatagcagtt
4201 cttgcaagga agcaaagttt ttcaacggtt tgagaccgtc cgccgtaggc atgcttttga
4261 gcgtttgacc aagcagttcc aggcggtccc acagctcggt cacctgctct acggcatctc
4321 gatccagcat atctcctcgt ttcgcgggtt ggggcggctt tcgctgtacg gcagtagtcg
4381 gtgctcgtcc agacgggcca gggtcatgtc tttccacggg cgcagggtcc tcgtcagcgt
4441 agtctgggtc acggtgaagg ggtgcgctcc gggctgcgcg ctggccaggg tgcgcttgag
4501 gctggtcctg ctggtgctga agcgctgccg gtcttcgccc tgcgcgtcgg ccaggtagca
4561 tttgaccatg gtgtcatagt ccagcccctc cgcggcgtgg cccttggcgc gcagcttgcc
4621 cttggaggag gcgccgcacg aggggcagtg cagacttttg agggcgtaga gcttgggcgc
4681 gagaaatacc gattccgggg agtaggcatc cgcgccgcag gccccgcaga cggtctcgca
4741 ttccacgagc caggtgagct ctggccgttc ggggtcaaaa accaggtttc ccccatgctt
4801 tttgatgcgt ttcttacctc tggtttccat gagccggtgt ccacgctcgg tgacgaaaag
4861 gctgtccgtg tccccgtata cagacttgag aggcctgtcc tcgagcggtg ttccgcggtc
4921 ctcctcgtat agaaactcgg accactctga gacaaaggct cgcgtccagg ccagcacgaa
4981 ggaggctaag tgggaggggt agcggtcgtt gtccactagg gggtccactc gctccagggt
5041 gtgaagacac atgtcgccct cttcggcatc aaggaaggtg attggtttgt aggtgtaggc
5101 cacgtgaccg ggtgttcctg aaggggggct ataaaagggg gtgggggcgc gttcgtcctc
5161 actctcttcc gcatcgctgt ctgcgagggc cagctgttgg ggtgagtact ccctctgaaa
5221 agcgggcatg acttctgcgc taagattgtc agtttccaaa aacgaggagg atttgatatt
5281 cacctggccc gcggtgatgc ctttgagggt ggccgcatcc atctggtcag aaaagacaat
5341 ctttttgttg tcaagctagc ttctatgggt ttaacaatac ctgtgtggaa gcctggaccg
5401 atgtaagggt tcggggctgt gccttttact gctgctggaa gggggtggtg tgtcgcccca
5461 aaagcagggc ttcaattaag aaatgcctct ttgaaaggtg taccttgggt atcctgtctg
5521 agggtaactc cagggtgcgc cacaatgtgg cctccgactg tggttgcttc atgctagtga
5581 aaagcgtggc tgtgattaag cataacatgg tatgtggcaa ctgcgaggac agggcctctc
5641 agatgctgac ctgctcggac ggcaactgtc acctgctgaa gaccattcac gtagccagcc
5701 actctcgcaa ggcctggcca gtgtttgagc ataacatact gacccgctgt tccttgcatt
5761 tgggtaacag gaggggggtg ttcctacctt accaatgcaa tttgagtcac actaagatat
5821 tgcttgagcc cgagagcatg tccaaggtga acctgaacgg ggtgtttgac atgaccatga
5881 agatctgcgt ctccggtctc gccgcttaga tcgctctgtg tagtagttgt agtatatcca
5941 ctctctcaaa gcatccaggc gccccctggc ttcgggttct atgtaaactc cttcatgcgc
6001 cgctgccctg ataacatcca ccaccgcaga ataagccaca cccagccaac ctacacattc
6061 gttctgcgag tcacacacgg gaggagcggg aagagctgga agaaccatgt tttttttttt
6121 attccaaaag attatccaaa acctcaaaat gaagatcctc tacgccggac gcatcgtggc
6181 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg
6241 ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc
6301 aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc
6361 ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa
6421 gggagagcgt cgatcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt
6481 gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg
6541 taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg
6601 cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct
6661 tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg
6721 cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc
6781 ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt
6841 ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg gctcttacca
6901 gcctaacttc gatcactgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca
6961 catggaacgg gttggcatgg attgtaggcg ccgccctata ccttgtctgc ctccccgcgt
7021 tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat ggaagccggc ggcacctcgc
7081 taacggattc accactccaa gaattggagc caatcaattc ttgcggagaa ctgtgaatgc
7141 gcaaaccaac ccttggcaga acatatccat cgcgtccgcc atctccagca gccgcacgcg
7201 gcgcatctcg ggcagcgttg ggtcctggcc acgggtgcgc atgatcgtgc tcctgtcgtt
7261 gaggacccgg ctaggctggc ggggttgcct tactggttag cagaatgaat caccgatacg
7321 cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa caacatgaat
7381 ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc tcttccgctt
7441 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
7501 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
7561 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
7621 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
7681 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
7741 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
7801 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
7861 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
7921 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
7981 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
8041 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
8101 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
8161 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
8221 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
8281 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
8341 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
8401 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
8461 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
8521 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
8581 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
8641 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg
8701 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
8761 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
8821 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
8881 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
8941 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata
9001 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
9061 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
9121 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
9181 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
9241 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
9301 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
9361 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
9421 ggccctttcg tcttcaagaa ttaattc
//