LOCUS LT727054 6521 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-E-sptP, complete sequence.
ACCESSION LT727054
VERSION LT727054.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-E-sptP
ORGANISM Mammalian expression vector pCAGGS-E-sptP
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6521)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6521
/organism="Mammalian expression vector pCAGGS-E-sptP"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4878."
/db_xref="taxon:1944819"
misc_feature 16..54
/note="E-tag"
CDS 64..1695
/transl_table=11
/note="sptP"
/protein_id="SJL87302.1"
/translation="MLKYEERKLNNLTLSSFSKVGVSNDARLYIAKENTDKAYVAPEK
FSSKVLTWLGKMPLFKNTEVVQKHTENIRVQDQKILQTFLHALTEKYGETAVNDALLM
SRINMNKPLTQRLAVQITECVKAADEGFINLIKSKDNVGVRNAALVIKGGDTKVAEKN
NDVGAESKQPLLDIALKGLKRTLPQLEQMDGNSLRENFQEMASGNGPLRSLMTNLQNL
NKIPEAKQLNDYVTTLTNIQVGVARFSQWGTCGGEVERWVDKASTHELTQAVKKIHVI
AKELKNVTAELEKIEAGAPMPQTMSGPTLGLARFAVSSIPINQQTQVKLSDGMPVPVN
TLTFDGKPVALAGSYPKNTPDALEAHMKMLLEKECSCLVVLTSEDQMQAKQLPPYFRG
SYTFGEVHTNSQKVSSASQGEAIDQYNMQLSCGEKRYTIPVLHVKNWPDHQPLPSTDQ
LEYLADRVKNSNQNGAPGRSSSDKHLPMIHCLGGVGRTGTMAAALVLKDNPHSNLEQV
RADFRDSRNNRMLEDASQFVQLKAMQAQLLMTTAS"
regulatory 1811..1902
/note="3' UTR of rabbit beta-globin, incl. polyA"
/regulatory_class="terminator"
misc_feature 1903..2259
/note="3' flanking region of rabbit beta-globin"
regulatory complement(2337..2419)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 2561..2636
/note="SV40 ori"
regulatory 2667..2741
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(2667..2741)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(2856..3257)
/note="pMB1 ori"
CDS complement(3798..4658)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87303.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4794..5171
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 5177..6518
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 5546..6470
/note="intron AG promoter"
BASE COUNT 1557 a 1619 c 1770 g 1575 t
ORIGIN
1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
61 gctatgctaa agtatgagga gagaaaattg aataatttaa cgttgtcttc gttttcaaaa
121 gttggtgtgt cgaatgatgc ccgactttat attgctaagg aaaatactga taaggcatat
181 gttgcgcctg aaaaattttc gtcaaaagta ttaacctggc ttggaaaaat gccgttattt
241 aaaaacactg aagtggtgca aaaacatacg gaaaatatca gagtacagga ccaaaagatt
301 ttacagacat ttctccatgc actaacggaa aaatatgggg aaacagcggt taatgacgca
361 ctgttaatgt cccgtataaa tatgaacaaa ccccttaccc aacgtttagc agtgcagatc
421 acggagtgtg taaaagctgc tgacgaaggg tttataaacc ttattaagag caaggataat
481 gttggtgtca ggaatgccgc tttagtcata aaaggcggcg atacaaaagt ggcagaaaaa
541 aataacgatg ttggagcaga aagtaagcaa cctttactcg atatcgcgct aaagggactt
601 aaaagaacat taccgcaact ggaacaaatg gatggaaata gcctgcgaga aaacttccag
661 gagatggctt caggtaacgg cccgctgcgt tcattaatga cgaatttaca gaatttaaat
721 aaaattccag aggctaaaca acttaatgat tatgttacaa cgttaacaaa tattcaggtc
781 ggtgtagccc ggttctcaca atggggaacc tgtggaggag aggttgagag gtgggttgat
841 aaagcctcta ctcatgaatt aacccaggct gtaaaaaaaa ttcacgttat tgctaaagaa
901 ctgaaaaatg ttacagctga acttgagaaa atagaagccg gcgcgccaat gccacagacg
961 atgagcggac cgacactggg cctggcacga tttgcggtca gcagcattcc aattaatcag
1021 caaacccagg tgaaactgag cgatggaatg cctgtgccag tgaatacgtt aacttttgac
1081 ggtaagcctg tggcattagc cggttcgtac ccaaaaaata cgccggatgc gctggaagcg
1141 catatgaaaa tgcttcttga aaaagaatgc tcgtgcctgg tggtgttaac gtcggaagat
1201 cagatgcagg caaaacaatt accaccctat ttcagaggga gctacacctt tggcgaggtg
1261 cataccaaca gccaaaaagt gagctcagcc agtcagggag aagcgataga ccaatacaat
1321 atgcaactgt cctgcgggga aaagcggtat acaatcccgg tattgcatgt gaaaaattgg
1381 ccagatcacc agccgttacc gtctacggat cagttagaat acctggcgga tagggtgaaa
1441 aatagtaacc aaaatggcgc gccggggcgt agttcatcag ataagcattt accgatgatt
1501 cattgtctgg gcggagtggg aagaaccgga acgatggcgg cggcccttgt acttaaggat
1561 aatcctcata gtaatctgga gcaggtacgt gcagatttcc gggattcacg gaacaataga
1621 atgctggaag acgcctcaca gtttgtacaa ctaaaagcaa tgcaagccca gttgcttatg
1681 acgacggcaa gctgacccgg gacattaaac gcgtgggtac cggatccggc aattcactcc
1741 tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa
1801 ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga
1861 gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
1921 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga
1981 gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg
2041 gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag
2101 aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt
2161 aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact
2221 actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct
2281 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
2341 acaacatacg agccggaagc ataaagtgta aagcctgggt gcctaatgag tgagctaact
2401 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg
2461 gatccgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
2521 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
2581 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
2641 ggaggcctag gcttttgcaa aaagctaact tgtttattgc agcttataat ggttacaaat
2701 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
2761 gtttgtccaa actcatcaat gtatcttatc atgtctggat ccgctgcatt aatgaatcgg
2821 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
2881 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
2941 acggttatcc acagaatcag ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
3001 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
3061 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
3121 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
3181 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
3241 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
3301 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
3361 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
3421 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
3481 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
3541 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
3601 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
3661 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
3721 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
3781 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
3841 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
3901 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
3961 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
4021 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
4081 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
4141 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
4201 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
4261 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
4321 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
4381 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
4441 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
4501 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
4561 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
4621 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
4681 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
4741 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgg gtcgacattg
4801 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
4861 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
4921 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
4981 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
5041 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
5101 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
5161 cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc
5221 ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc
5281 gggggggggg ggggggcccc ccccaggcgg ggcggggcgg ggcgaggggc ggggcggggc
5341 gaggcggaaa ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat
5401 ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgt
5461 tgcgcgctgc cttccccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct
5521 ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg
5581 taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga
5641 ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt
5701 gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg
5761 cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc
5821 cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg
5881 ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc
5941 ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg
6001 gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc
6061 tcgggccggg gagggctcgg gggaaggggc gcggcggccc ccggagcgcc ggcggctgtc
6121 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac
6181 ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta
6241 gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc
6301 gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg
6361 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
6421 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
6481 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga a
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