LOCUS LT727054 6521 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian expression vector pCAGGS-E-sptP, complete sequence. ACCESSION LT727054 VERSION LT727054.1 KEYWORDS . SOURCE Mammalian expression vector pCAGGS-E-sptP ORGANISM Mammalian expression vector pCAGGS-E-sptP other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 6521) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..6521 /organism="Mammalian expression vector pCAGGS-E-sptP" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 4878." /db_xref="taxon:1944819" misc_feature 16..54 /note="E-tag" CDS 64..1695 /transl_table=11 /note="sptP" /protein_id="SJL87302.1" /translation="MLKYEERKLNNLTLSSFSKVGVSNDARLYIAKENTDKAYVAPEK FSSKVLTWLGKMPLFKNTEVVQKHTENIRVQDQKILQTFLHALTEKYGETAVNDALLM SRINMNKPLTQRLAVQITECVKAADEGFINLIKSKDNVGVRNAALVIKGGDTKVAEKN NDVGAESKQPLLDIALKGLKRTLPQLEQMDGNSLRENFQEMASGNGPLRSLMTNLQNL NKIPEAKQLNDYVTTLTNIQVGVARFSQWGTCGGEVERWVDKASTHELTQAVKKIHVI AKELKNVTAELEKIEAGAPMPQTMSGPTLGLARFAVSSIPINQQTQVKLSDGMPVPVN TLTFDGKPVALAGSYPKNTPDALEAHMKMLLEKECSCLVVLTSEDQMQAKQLPPYFRG SYTFGEVHTNSQKVSSASQGEAIDQYNMQLSCGEKRYTIPVLHVKNWPDHQPLPSTDQ LEYLADRVKNSNQNGAPGRSSSDKHLPMIHCLGGVGRTGTMAAALVLKDNPHSNLEQV RADFRDSRNNRMLEDASQFVQLKAMQAQLLMTTAS" regulatory 1811..1902 /note="3' UTR of rabbit beta-globin, incl. polyA" /regulatory_class="terminator" misc_feature 1903..2259 /note="3' flanking region of rabbit beta-globin" regulatory complement(2337..2419) /note="lac promoter" /regulatory_class="promoter" rep_origin 2561..2636 /note="SV40 ori" regulatory 2667..2741 /note="SV40 polyA early" /regulatory_class="terminator" regulatory complement(2667..2741) /note="SV40 polyA late" /regulatory_class="terminator" rep_origin complement(2856..3257) /note="pMB1 ori" CDS complement(3798..4658) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL87303.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" regulatory 4794..5171 /note="hCMV-IE enhancer" /regulatory_class="promoter" regulatory 5177..6518 /note="AG promoter" /regulatory_class="promoter" misc_feature 5546..6470 /note="intron AG promoter" BASE COUNT 1557 a 1619 c 1770 g 1575 t ORIGIN 1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 61 gctatgctaa agtatgagga gagaaaattg aataatttaa cgttgtcttc gttttcaaaa 121 gttggtgtgt cgaatgatgc ccgactttat attgctaagg aaaatactga taaggcatat 181 gttgcgcctg aaaaattttc gtcaaaagta ttaacctggc ttggaaaaat gccgttattt 241 aaaaacactg aagtggtgca aaaacatacg gaaaatatca gagtacagga ccaaaagatt 301 ttacagacat ttctccatgc actaacggaa aaatatgggg aaacagcggt taatgacgca 361 ctgttaatgt cccgtataaa tatgaacaaa ccccttaccc aacgtttagc agtgcagatc 421 acggagtgtg taaaagctgc tgacgaaggg tttataaacc ttattaagag caaggataat 481 gttggtgtca ggaatgccgc tttagtcata aaaggcggcg atacaaaagt ggcagaaaaa 541 aataacgatg ttggagcaga aagtaagcaa cctttactcg atatcgcgct aaagggactt 601 aaaagaacat taccgcaact ggaacaaatg gatggaaata gcctgcgaga aaacttccag 661 gagatggctt caggtaacgg cccgctgcgt tcattaatga cgaatttaca gaatttaaat 721 aaaattccag aggctaaaca acttaatgat tatgttacaa cgttaacaaa tattcaggtc 781 ggtgtagccc ggttctcaca atggggaacc tgtggaggag aggttgagag gtgggttgat 841 aaagcctcta ctcatgaatt aacccaggct gtaaaaaaaa ttcacgttat tgctaaagaa 901 ctgaaaaatg ttacagctga acttgagaaa atagaagccg gcgcgccaat gccacagacg 961 atgagcggac cgacactggg cctggcacga tttgcggtca gcagcattcc aattaatcag 1021 caaacccagg tgaaactgag cgatggaatg cctgtgccag tgaatacgtt aacttttgac 1081 ggtaagcctg tggcattagc cggttcgtac ccaaaaaata cgccggatgc gctggaagcg 1141 catatgaaaa tgcttcttga aaaagaatgc tcgtgcctgg tggtgttaac gtcggaagat 1201 cagatgcagg caaaacaatt accaccctat ttcagaggga gctacacctt tggcgaggtg 1261 cataccaaca gccaaaaagt gagctcagcc agtcagggag aagcgataga ccaatacaat 1321 atgcaactgt cctgcgggga aaagcggtat acaatcccgg tattgcatgt gaaaaattgg 1381 ccagatcacc agccgttacc gtctacggat cagttagaat acctggcgga tagggtgaaa 1441 aatagtaacc aaaatggcgc gccggggcgt agttcatcag ataagcattt accgatgatt 1501 cattgtctgg gcggagtggg aagaaccgga acgatggcgg cggcccttgt acttaaggat 1561 aatcctcata gtaatctgga gcaggtacgt gcagatttcc gggattcacg gaacaataga 1621 atgctggaag acgcctcaca gtttgtacaa ctaaaagcaa tgcaagccca gttgcttatg 1681 acgacggcaa gctgacccgg gacattaaac gcgtgggtac cggatccggc aattcactcc 1741 tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa 1801 ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga 1861 gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt 1921 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga 1981 gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg 2041 gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag 2101 aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt 2161 aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact 2221 actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct 2281 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 2341 acaacatacg agccggaagc ataaagtgta aagcctgggt gcctaatgag tgagctaact 2401 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg 2461 gatccgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc 2521 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat 2581 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt 2641 ggaggcctag gcttttgcaa aaagctaact tgtttattgc agcttataat ggttacaaat 2701 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 2761 gtttgtccaa actcatcaat gtatcttatc atgtctggat ccgctgcatt aatgaatcgg 2821 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 2881 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 2941 acggttatcc acagaatcag ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 3001 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 3061 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 3121 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 3181 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 3241 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 3301 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 3361 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 3421 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga 3481 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 3541 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 3601 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 3661 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 3721 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 3781 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 3841 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 3901 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 3961 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 4021 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 4081 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 4141 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 4201 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 4261 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 4321 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 4381 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 4441 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 4501 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 4561 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 4621 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 4681 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 4741 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgg gtcgacattg 4801 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat 4861 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc 4921 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca 4981 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta 5041 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta 5101 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat 5161 cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc 5221 ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc 5281 gggggggggg ggggggcccc ccccaggcgg ggcggggcgg ggcgaggggc ggggcggggc 5341 gaggcggaaa ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat 5401 ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgt 5461 tgcgcgctgc cttccccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct 5521 ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg 5581 taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga 5641 ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt 5701 gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg 5761 cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc 5821 cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg 5881 ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc 5941 ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg 6001 gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc 6061 tcgggccggg gagggctcgg gggaaggggc gcggcggccc ccggagcgcc ggcggctgtc 6121 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac 6181 ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta 6241 gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc 6301 gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg 6361 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg 6421 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca 6481 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga a //