LOCUS LT727053 5612 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian expression vector pCAGGS-E-sopE2, complete sequence. ACCESSION LT727053 VERSION LT727053.1 KEYWORDS . SOURCE Mammalian expression vector pCAGGS-E-sopE2 ORGANISM Mammalian expression vector pCAGGS-E-sopE2 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 5612) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..5612 /organism="Mammalian expression vector pCAGGS-E-sopE2" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 4877." /db_xref="taxon:1944817" misc_feature 16..54 /note="E-tag" CDS 64..786 /transl_table=11 /note="sopE2" /protein_id="SJL87300.1" /translation="MTNITLSTQHYRIHRSDVEPVKEKTTEKDIFAKSITAVRNSFIS LSTSLSDRFSLHQQTDIPTTHFHRGNASEGRAVLTSKTVKDFMLQKLNSLDIKGNASK DPAYARQTCEAILSAVYSNNKDQCCKLLISKGVSITPFLKEIGEAAQNAGLPGEIKNG VFTPGGAGANPFVVPLIASASIKYPHMFINHNQQVSFKAYAEKIVMKEVTPLFNKGTM PTPQQFQLTIENIANKYLQNAS" regulatory 902..993 /note="3' UTR of rabbit beta-globin, incl. polyA" /regulatory_class="terminator" misc_feature 994..1350 /note="3' flanking region of rabbit beta-globin" regulatory complement(1428..1510) /note="lac promoter" /regulatory_class="promoter" rep_origin 1652..1727 /note="SV40 ori" regulatory 1758..1832 /note="SV40 polyA early" /regulatory_class="terminator" regulatory complement(1758..1832) /note="SV40 polyA late" /regulatory_class="terminator" rep_origin complement(1947..2348) /note="pMB1 ori" CDS complement(2889..3749) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL87301.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" regulatory 3885..4262 /note="hCMV-IE enhancer" /regulatory_class="promoter" regulatory 4268..5609 /note="AG promoter" /regulatory_class="promoter" misc_feature 4637..5561 /note="intron AG promoter" BASE COUNT 1253 a 1446 c 1524 g 1389 t ORIGIN 1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 61 gctatgacta acataacact atccacccag cactacagaa tccatagaag tgacgttgaa 121 ccagtaaaag aaaaaacaac ggagaaggac atttttgcaa aaagtattac tgccgttaga 181 aatagcttta tcagcctgtc gacgagtctg tcagatcgtt ttagcctgca tcaacaaaca 241 gacataccga ctacccattt tcatcgtggg aacgcttctg agggtagggc ggtattaacc 301 agtaaaactg ttaaagattt tatgctgcaa aagctcaata gtctggatat caaaggtaat 361 gcgagtaaag atccggccta tgctcgtcag acatgcgaag ccatattatc agccgtgtac 421 agtaataata aagatcaatg ttgtaaatta ctcatcagta aaggggtcag tattaccccc 481 tttttgaaag agataggaga ggctgcgcag aatgcggggc tacctgggga gataaaaaat 541 ggcgtattta ctccaggtgg ggcaggggcg aatccttttg tcgtccccct cattgcttcc 601 gcgagtatta aatatccgca tatgtttata aatcataatc agcaggtatc ttttaaagcg 661 tatgctgaga aaatcgttat gaaagaggtt acgccgctgt ttaataaggg gacgatgcca 721 acgccacaac aatttcagtt aactatagaa aatattgcga ataagtatct tcagaatgcc 781 tcctgacccg ggacattaaa cgcgtgggta ccggatccgg caattcactc ctcaggtgca 841 ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg 901 agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac 961 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct 1021 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt 1081 ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag 1141 aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt 1201 gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct 1261 aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt 1321 catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc ttggcgtaat 1381 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 1441 gagccggaag cataaagtgt aaagcctggg tgcctaatga gtgagctaac tcacattaat 1501 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc ggatccgcat 1561 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg 1621 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 1681 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 1741 ggcttttgca aaaagctaac ttgtttattg cagcttataa tggttacaaa taaagcaata 1801 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 1861 aactcatcaa tgtatcttat catgtctgga tccgctgcat taatgaatcg gccaacgcgc 1921 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 1981 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 2041 cacagaatca gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 2101 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 2161 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 2221 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 2281 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 2341 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 2401 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 2461 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 2521 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 2581 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 2641 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 2701 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 2761 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 2821 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 2881 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 2941 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 3001 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 3061 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 3121 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 3181 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 3241 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 3301 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 3361 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 3421 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 3481 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 3541 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 3601 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 3661 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 3721 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 3781 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 3841 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ggtcgacatt gattattgac 3901 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 3961 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 4021 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 4081 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 4141 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 4201 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 4261 catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc 4321 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg 4381 gggggggccc cccccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggaa 4441 aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg 4501 gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ttgcgcgctg 4561 ccttcccccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac 4621 cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg 4681 cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg aggggctccg 4741 ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg 4801 gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg 4861 gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg 4921 cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc 4981 agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc cccgagttgc 5041 tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt 5101 gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc ctcgggccgg 5161 ggagggctcg ggggaagggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg 5221 cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt 5281 cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg 5341 gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc 5401 cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc 5461 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag 5521 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg 5581 ttattgtgct gtctcatcat tttggcaaag aa //