LOCUS LT727051 6668 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-E-sipB, complete sequence.
ACCESSION LT727051
VERSION LT727051.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-E-sipB
ORGANISM Mammalian expression vector pCAGGS-E-sipB
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6668)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6668
/organism="Mammalian expression vector pCAGGS-E-sipB"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4874."
/db_xref="taxon:1944815"
misc_feature 16..54
/note="E-tag"
CDS <64..1842
/codon_start=1
/transl_table=11
/note="sipB"
/protein_id="SJL87296.1"
/translation="VNDASSISRSGYTQNPRLAEAAFEGVRKNTDFLKAADKAFKDVV
ATKAGDLKAGTKSGESAINTVGLKPPTDAAREKLSSEGQLTLLLGKLMTLLGDVSLSQ
LESRLAVWQAMIESQKEMGIQVSKEFQTALGEAQEATDLYEASIKKTDTAKSVYDAAT
KKLTQAQNKLQSLDPADPGYAQAEAAVEQAGKEATEAKEALDKATDATVKAGTDAKAK
AEKADNILTKFQGTANAASQNQVSQGEQDNLSNVARLTMLMAMFIEIVGKNTEESLQN
DLALFNALQEGRQAEMEKKSAEFQEETRKAEETNRIMGCIGKVLGALLTIVSVVAAVF
TGGASLALAAVGLAVMVADEIVKAATGVSFIQQALNPIMEHVLKPLMELIGKAITKAL
EGLGVDKKTAEMAGSIVGAIVAAIAMVAVIVVVAVVGKGAAAKLGNALSKMMGETIKK
LVPNVLKQLAQNGSKLFTQGMQRITSGLGNVGSKMGLQTNALSKELVGNTLNKVALGM
EVTNTAAQSAGGVAEGVFIKNASEALADFMLARFAMDQIQQWLKQSVEIFGENQKVTA
ELQKAMSSAVQQNADASRFILRQSRA"
regulatory 1958..2049
/note="3' UTR of rabbit beta-globin, incl. polyA"
/regulatory_class="terminator"
misc_feature 2050..2406
/note="3' flanking region of rabbit beta-globin"
regulatory complement(2484..2566)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 2708..2783
/note="SV40 ori"
regulatory complement(2814..2888)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 2814..2888
/note="SV40 polyA early"
/regulatory_class="terminator"
rep_origin complement(3003..3404)
/note="pMB1 ori"
CDS complement(3945..4805)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87297.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4941..5318
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 5324..6665
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 5693..6617
/note="intron AG promoter"
BASE COUNT 1504 a 1689 c 1911 g 1564 t
ORIGIN
1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
61 gctgtaaatg acgcaagtag cattagccgt agcggatata cccaaaatcc gcgcctcgct
121 gaggcggctt ttgaaggcgt tcgtaagaac acggactttt taaaagcggc ggataaagct
181 tttaaagatg tggtggcaac gaaagcgggc gaccttaaag ccggaacaaa gtccggcgag
241 agcgctatta atacggtggg tctaaagccg cctacggacg ccgcccggga aaaactctcc
301 agcgaagggc aattgacatt actgcttggc aagttaatga ccctactggg cgatgtttcg
361 ctgtctcaac tggagtctcg tctggcggta tggcaggcga tgattgagtc acaaaaagag
421 atggggattc aggtatcgaa agaattccag acggctctgg gagaggctca ggaggcgacg
481 gatctctatg aagccagtat caaaaagacg gataccgcca agagtgttta tgacgctgcg
541 accaaaaaac tgacgcaggc gcaaaataaa ttgcaatcgc tggacccggc tgaccccggc
601 tatgcacaag ctgaagccgc ggtagaacag gccggaaaag aagcgacaga ggcgaaagag
661 gccttagata aggccacgga tgcgacggtt aaagcaggca cagacgccaa agcgaaagcc
721 gagaaagcgg ataacattct gaccaaattc cagggaacgg ctaatgccgc ctctcagaat
781 caggtttccc agggtgagca ggataatctg tcaaatgtcg cccgcctcac tatgctcatg
841 gccatgttta ttgagattgt gggcaaaaat acggaagaaa gcctgcaaaa cgatcttgcg
901 cttttcaacg ccttgcagga agggcgtcag gcggagatgg aaaagaaatc ggctgaattc
961 caggaagaga cgcgcaaagc cgaggaaacg aaccgcatta tgggatgtat cgggaaagtc
1021 ctcggcgcgc tgctaaccat tgtcagcgtt gtggccgctg tttttaccgg tggggcgagt
1081 ctggcgctgg ctgcggtggg acttgcggta atggtggccg atgaaattgt gaaggcggcg
1141 acgggagtgt cgtttattca gcaggcgcta aacccgatta tggagcatgt gctgaagccg
1201 ttaatggagc tgattggcaa ggcgattacc aaagcgctgg aaggattagg cgtcgataag
1261 aaaacggcag agatggccgg cagcattgtt ggtgcgattg tcgccgctat tgccatggtg
1321 gcggtcattg tggtggtcgc agttgtcggg aaaggcgcgg cggcgaaact gggtaacgcg
1381 ctgagcaaaa tgatgggcga aacgattaag aagttggtgc ctaacgtgct gaaacagttg
1441 gcgcaaaacg gcagcaaact ctttacccag gggatgcaac gtattactag cggtctgggt
1501 aatgtgggta gcaagatggg cctgcaaacg aatgccttaa gtaaagagct ggtaggtaat
1561 accctaaata aagtggcgtt gggcatggaa gtcacgaata ccgcagccca gtcagccggt
1621 ggtgttgccg agggcgtatt tattaaaaat gccagcgagg cgcttgctga ttttatgctc
1681 gcccgttttg ccatggatca gattcagcag tggcttaaac aatccgtaga aatatttggt
1741 gaaaaccaga aggtaacggc ggaactgcaa aaagccatgt cttctgcggt acagcaaaat
1801 gcggatgctt cgcgttttat tctgcgccag agtcgcgcat aacccgggac attaaacgcg
1861 tgggtaccgg atccggcaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc
1921 tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa
1981 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat
2041 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
2101 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca
2161 tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc
2221 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat
2281 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact
2341 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg
2401 gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
2461 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
2521 cctgggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
2581 ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc
2641 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
2701 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
2761 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt
2821 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
2881 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
2941 tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
3001 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
3061 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcaggga taacgcagga
3121 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
3181 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
3241 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
3301 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
3361 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
3421 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
3481 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
3541 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
3601 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
3661 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
3721 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
3781 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
3841 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
3901 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
3961 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
4021 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
4081 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
4141 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
4201 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
4261 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
4321 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
4381 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
4441 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
4501 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
4561 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
4621 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
4681 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
4741 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
4801 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
4861 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
4921 cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt aatcaattac
4981 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
5041 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
5101 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
5161 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
5221 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
5281 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt
5341 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt
5401 ttaattattt tgtgcagcga tgggggcggg gggggggggg gggccccccc caggcggggc
5461 ggggcggggc gaggggcggg gcggggcgag gcggaaaggt gcggcggcag ccaatcagag
5521 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa
5581 gcgaagcgcg cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc cccgctccgc
5641 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg
5701 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc
5761 ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag
5821 cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct
5881 gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg
5941 cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa
6001 aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg
6061 gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc
6121 ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt
6181 gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aaggggcgcg
6241 gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat
6301 ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat
6361 ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca
6421 ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc
6481 tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg
6541 ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct
6601 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg
6661 gcaaagaa
//