LOCUS LT727050 5929 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-mCASP-7a, complete sequence.
ACCESSION LT727050
VERSION LT727050.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-mCASP-7a
ORGANISM Mammalian expression vector pCAGGS-mCASP-7a
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5929)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5929
/organism="Mammalian expression vector pCAGGS-mCASP-7a"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4868."
/db_xref="taxon:1944888"
misc_feature complement(62..72)
/note="3UTR"
CDS complement(73..984)
/transl_table=11
/note="CASP7"
/protein_id="SJL87294.1"
/translation="MTDDQDCAAELEKVDSSSEDGVDAKPDRSSIISSILLKKKRNAS
AGPVRTGRDRVPTYLYRMDFQKMGKCIIINNKNFDKATGMDVRNGTDKDAGALFKCFQ
NLGFEVTVHNDCSCAKMQDLLRKASEEDHSNSACFACVLLSHGEEDLIYGKDGVTPIK
DLTAHFRGDRCKTLLEKPKLFFIQACRGTELDDGIQADSGPINDIDANPRNKIPVEAD
FLFAYSTVPGYYSWRNPGKGSWFVQALCSILNEHGKDLEIMQILTRVNDRVARHFESQ
SDDPRFNEKKQIPCMVSMLTKELYFSR"
misc_feature complement(985..1173)
/note="5UTR"
regulatory 1219..1310
/note="polyA"
/regulatory_class="terminator"
misc_feature 1311..1667
/note="3FR"
regulatory complement(1745..1827)
/note="lac"
/regulatory_class="promoter"
rep_origin 1969..2044
/note="SV40 ori"
regulatory 2075..2149
/note="polyA"
/regulatory_class="terminator"
regulatory complement(2075..2149)
/note="polyA"
/regulatory_class="terminator"
rep_origin complement(2264..2665)
/note="pMB1 ori"
CDS complement(3206..4066)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87295.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
misc_feature 4202..4579
/note="hCMV"
regulatory 4585..5926
/note="AG"
/regulatory_class="promoter"
misc_feature 4954..5878
/note="intron"
BASE COUNT 1228 a 1581 c 1671 g 1449 t
ORIGIN
1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
61 gctgaagggt ggtcaacggc tgaagtacag ctctttggtg agcatggaca ccatacacgg
121 gatctgcttc ttctcgttga agcgtggatc atcagactgg gactcgaagt gcctggccac
181 cctgtcgttc accctggtca ggatctgcat gatctcgagg tccttgccat gctcattcag
241 gatggagcag agggcctgca caaaccagga gcctttccct gggttcctcc atgagtaata
301 acctggaacc gtggagtaag caaagaggaa gtcggcttcc accgggatct tgttgcgggg
361 attagcgtca atgtcgttga tgggccccga gtcagcctgg attccatcat cgagctccgt
421 ccctcggcat gcctgaatga agaagagttt gggtttctct aacagggttt tgcatcggtc
481 tcccctaaaa tgagctgtca gatcctttat gggtgtcacg ccatctttcc cgtaaatcag
541 gtcctcttcc ccgtggctca gcaggacgca ggcgaagcag gccgagttgc tgtggtcctc
601 ctcagaggct tttctaagca gatcttgcat ctttgcacaa gagcagtcat tgtggacggt
661 tacttcaaaa cccaggtttt ggaagcactt gaagagggcc cctgcatctt tgtccgtccc
721 attccggacg tccatacctg tcgctttgtc gaagttcttg ttgtttatga tgatgcattt
781 acccatcttc tggaaatcca tgcggtacag ataagtgggc actcggtccc ggccggtcct
841 gacggggccc gcagaggcat ttctcttctt cttcaagaga atagaggaga tgatagagga
901 gcggtctggc ttggcgtcaa ctccgtcttc gctggaagaa tccacctttt ccagctccgc
961 agcacagtcc tgatcatcgg tcatcgttcc cacggcggcg gccaggagcc cacggcagcg
1021 tccgtcctcc gcaagggtcg agggcagctt tgggaaaact gaaagcaaaa gtcccggagg
1081 cggaggggga ggagcgggcg ggccggacgg ggctgtgcct gcagcccggg aaagctgggg
1141 gcgagcggga acgcgcgcta cggagcccgc cgcctcgtgc cgaattcccc caatgccctg
1201 gctcacaaat accactgaga tctttttccc tctgccaaaa attatgggga catcatgaag
1261 ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt
1321 tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat
1381 cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac
1441 aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta
1501 ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt
1561 ttttctttaa catccctaaa attttcctta catgttttac tagccagatt tttcctcctc
1621 tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct cgacctgcag
1681 cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
1741 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctgggtgc ctaatgagtg
1801 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
1861 tgccagcgga tccgcatctc aattagtcag caaccatagt cccgccccta actccgccca
1921 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
1981 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
2041 gcttttttgg aggcctaggc ttttgcaaaa agctaacttg tttattgcag cttataatgg
2101 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
2161 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc gctgcattaa
2221 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
2281 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
2341 gcggtaatac ggttatccac agaatcaggg ataacgcagg aaagaacatg tgagcaaaag
2401 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
2461 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
2521 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
2581 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
2641 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
2701 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
2761 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
2821 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
2881 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
2941 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
3001 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
3061 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
3121 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
3181 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
3241 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
3301 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
3361 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
3421 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
3481 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
3541 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
3601 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
3661 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
3721 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
3781 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
3841 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
3901 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
3961 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
4021 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
4081 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
4141 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgggt
4201 cgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
4261 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
4321 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
4381 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
4441 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
4501 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
4561 ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc
4621 tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg
4681 atgggggcgg gggggggggg ggggcccccc ccaggcgggg cggggcgggg cgaggggcgg
4741 ggcggggcga ggcggaaagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
4801 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
4861 ggagtcgttg cgcgctgcct tccccccgtg ccccgctccg ccgccgcctc gcgccgcccg
4921 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct
4981 ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa
5041 agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc
5101 gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc
5161 tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg
5221 ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
5281 tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac
5341 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt
5401 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
5461 gggccgcctc gggccgggga gggctcgggg gaaggggcgc ggcggccccc ggagcgccgg
5521 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
5581 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
5641 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
5701 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
5761 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
5821 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
5881 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaa
//