LOCUS LT727048 6640 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-FLAGmA20d5, complete sequence.
ACCESSION LT727048
VERSION LT727048.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-FLAGmA20d5
ORGANISM Mammalian expression vector pCAGGS-FLAGmA20d5
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6640)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6640
/organism="Mammalian expression vector pCAGGS-FLAGmA20d5"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4844."
/db_xref="taxon:1944853"
misc_feature 13..36
/note="FLAG epitope"
CDS 46..1914
/transl_table=11
/note="mA20 (d547-699)"
/protein_id="SJL87289.1"
/translation="MAEQLLPQALYLSNMRKAVKIRERTPEDIFKPTNGIIYHFKTMH
RYTLEMFRTCQFCPQFREIIHKALIDRSVQASLESQKKLNWCREVRKLVALKTNGDGN
CLMHAACQYMWGVQDTDLVLRKALCSTLKETDTRNFKFRWQLESLKSQEFVETGLCYD
TRNWNDEWDNLVKMASADTPAARSGLQYNSLEEIHIFVLSNILRRPIIVISDKMLRSL
ESGSNFAPLKVGGIYLPLHWPAQECYRYPIVLGYDSQHFVPLVTLKDSGPELRAVPLV
NRDRGRFEDLKVHFLTDPENEMKEKLLKEYLIVMEIPVQGWDHGTTHLINAAKLDEAN
LPKEINLVDDYFELVQHEYKKWQENSDQARRAAHAQNPLEPSTPQLSLMDIKCETPNC
PFFMSVNTQPLCHECSERRQKNQSKLPKLNSKLGPEGLPGVGLGSSNWSPEETAGGPH
SAPPTAPSLFLFSETTAMKCRSPGCPFTLNVQHNGFCERCHARQINASHTADPGKCQA
CLQDVTRTFNGICSTCFKRTTAEPSSSLTSSIPASCHQKCARASCKNILACRSEELCM
ECQHLSQRVGSVAHRGEPTPEEPPKQRCRAPACDHFGNAKCNGYCNECYQFKQMYG"
regulatory 1926..2017
/note="3' UTR of rabbit beta-globin, incl. polyA"
/regulatory_class="terminator"
misc_feature 2018..2374
/note="3' flanking region of rabbit beta-globin"
regulatory complement(2452..2534)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 2676..2751
/note="SV40 ori"
regulatory 2782..2856
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(2782..2856)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(2971..3372)
/note="pMB1 ori"
CDS complement(3913..4773)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87290.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4913..5290
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 5296..6637
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 5665..6589
/note="intron AG promoter"
BASE COUNT 1511 a 1752 c 1780 g 1597 t
ORIGIN
1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccgccatggc tgaacaactt
61 cttcctcagg ctttgtattt gagcaatatg cggaaagctg tgaagatacg agagagaacc
121 ccagaagaca ttttcaaacc taccaatggg atcatctatc actttaaaac catgcaccga
181 tacacgctgg agatgttcag aacatgccag ttttgcccac agttccgaga gatcatccac
241 aaagcactta ttgacagaag tgtccaggct tccctggaaa gccagaagaa gctcaactgg
301 tgtcgtgaag tcaggaagct cgtggctctg aaaaccaatg gtgatggaaa ctgcctcatg
361 catgcagctt gtcagtacat gtggggtgtt caggatactg acctggtcct gaggaaggcc
421 ctctgcagca cccttaagga gacagacact cggaacttta aattccgctg gcagctggaa
481 tctctgaaat ctcaggaatt tgtggaaaca ggactttgct acgacactcg gaactggaat
541 gacgaatggg acaacttggt caaaatggca tcagcagaca cacctgcagc ccgaagtgga
601 cttcagtaca attccctgga agaaatccac atatttgtcc tcagcaacat cctcagaaga
661 cccatcattg tcatttcaga caaaatgcta agaagtttgg aatctggttc caattttgct
721 cctttgaaag tgggtgggat ttatctgcct cttcactggc ctgcccagga gtgttacaga
781 tatcccatcg tcctaggcta tgacagccag cactttgtac ccctggtgac cctgaaggac
841 agtggacctg aacttcgcgc tgttccactt gttaacagag accggggtag gtttgaagac
901 ttaaaagttc acttcttgac agatcctgag aatgagatga aggaaaagct tctaaaggag
961 tacttgatag tgatggagat ccctgtgcaa ggctgggacc acggcacgac tcacctgatc
1021 aacgctgcaa aattggatga agctaactta cccaaagaaa taaatttggt agacgattac
1081 tttgagcttg ttcagcacga atacaagaaa tggcaggaga acagcgatca ggccaggaga
1141 gcggcacatg cgcagaaccc cttggagcct tccacacccc agctatcact catggatata
1201 aaatgtgaga cacccaactg tcctttcttc atgtccgtga acactcagcc tttatgccac
1261 gaatgctcag agaggcgcca aaagaatcag agcaagctcc caaagctgaa ctcgaagcta
1321 ggccctgaag gactcccagg cgtgggactt ggctcctcaa actggagccc cgaggaaacc
1381 gctggaggac ctcattcagc cccacccaca gcacccagcc tttttctctt cagtgagacc
1441 actgcaatga agtgcaggag tcctgggtgc ccttttactt tgaatgtgca gcataatgga
1501 ttctgtgagc gttgccacgc ccggcagatt aatgccagcc acaccgcaga ccctggaaag
1561 tgccaagcct gccttcagga tgtcactcgg acctttaatg gcatctgcag tacctgtttc
1621 aaaaggacta cagcagagcc cagctccagc ctcacttcca gtatccctgc ctcctgtcac
1681 caaaaatgtg cccgggcctc ctgcaagaac attctggcct gtcgcagtga ggaactctgt
1741 atggagtgcc agcacctaag ccaacgagta ggttctgtgg cccaccgggg tgagcccacg
1801 cctgaagagc cccctaaaca gcgctgccgg gcccctgctt gtgatcactt tggcaatgcc
1861 aagtgtaatg gttactgcaa tgagtgctac cagttcaagc agatgtatgg ctaagtgtcg
1921 acgaagatct ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc
1981 tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt
2041 gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt
2101 tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat
2161 aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag
2221 ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat
2281 ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc
2341 cagtcatagc tgtccctctt ctcttatgga gatccctcga cctgcagccc aagcttggcg
2401 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
2461 atacgagccg gaagcataaa gtgtaaagcc tgggtgccta atgagtgagc taactcacat
2521 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc
2581 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
2641 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
2701 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
2761 cctaggcttt tgcaaaaagc taacttgttt attgcagctt ataatggtta caaataaagc
2821 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
2881 tccaaactca tcaatgtatc ttatcatgtc tggatccgct gcattaatga atcggccaac
2941 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
3001 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
3061 tatccacaga atcagggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
3121 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
3181 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
3241 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
3301 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
3361 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
3421 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
3481 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
3541 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
3601 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
3661 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
3721 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
3781 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
3841 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
3901 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
3961 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
4021 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
4081 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
4141 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
4201 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
4261 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
4321 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
4381 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
4441 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
4501 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
4561 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
4621 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
4681 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
4741 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
4801 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
4861 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga tcgacattga
4921 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
4981 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
5041 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
5101 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
5161 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
5221 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
5281 gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc
5341 tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg
5401 gggggggggg gggggccccc cccaggcggg gcggggcggg gcgaggggcg gggcggggcg
5461 aggcggaaag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg
5521 gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgtt
5581 gcgcgctgcc ttccccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc
5641 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt
5701 aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag
5761 gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg
5821 tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc
5881 ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc
5941 ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg
6001 gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc
6061 cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg
6121 ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct
6181 cgggccgggg agggctcggg ggaaggggcg cggcggcccc cggagcgccg gcggctgtcg
6241 aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact
6301 tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag
6361 cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg
6421 tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga
6481 cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg
6541 ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa
6601 cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
//