LOCUS LT727047 5747 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-FLAGAVPShOMI-S306A, complete
sequence.
ACCESSION LT727047
VERSION LT727047.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-FLAGAVPShOMI-S306A
ORGANISM Mammalian expression vector pCAGGS-FLAGAVPShOMI-S306A
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5747)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5747
/organism="Mammalian expression vector
pCAGGS-FLAGAVPShOMI-S306A"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4843."
/db_xref="taxon:1944826"
misc_feature 13..36
/note="FLAG epitope"
CDS 43..1023
/transl_table=11
/note="mature hOMI mutant"
/protein_id="SJL87287.1"
/translation="MAVPSPPPASPRSQYNFIADVVEKTAPAVVYIEILDRHPFLGRE
VPISNGSGFVVAADGLIVTNAHVVADRRRVRVRLLSGDTYEAVVTAVDPVADIATLRI
QTKEPLPTLPLGRSADVRQGEFVVAMGSPFALQNTITSGIVSSAQRPARDLGLPQTNV
EYIQTDAAIDFGNAGGPLVNLDGEVIGVNTMKVTAGISFAIPSDRLREFLHRGEKKNS
SSGISGSQRRYIGVMMLTLSPSILAELQLREPSFPDVQHGVLIHKVILGSPAHRAGLR
PGDVILAIGEQMVQNAEDVYEAVRTQSQLAVQIRRGRETLTLYVTPEVTE"
misc_feature 562..567
/note="S306A"
regulatory 1033..1124
/note="beta-globin polyA"
/regulatory_class="terminator"
misc_feature 1125..1481
/note="3' FR of beta-globin"
regulatory complement(1559..1641)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1783..1858
/note="SV40 ori"
regulatory 1889..1963
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(1889..1963)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(2078..2479)
/note="pMB1 ori"
CDS complement(3020..3880)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87288.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4020..4397
/note="hCMV enhancer"
/regulatory_class="promoter"
regulatory 4403..5744
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4772..5696
/note="intron AG promoter"
BASE COUNT 1209 a 1530 c 1607 g 1401 t
ORIGIN
1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccatggccgt ccctagcccg
61 ccgcccgctt ctccccggag tcagtacaac ttcatcgcag atgtggtgga gaagacagca
121 cctgccgtgg tctatatcga gatcctggac cggcaccctt tcttgggccg cgaggtccct
181 atctcgaacg gctcaggatt cgtggtggct gccgatgggc tcattgtcac caacgcccat
241 gtggtggctg atcggcgcag agtccgtgtg agactgctaa gcggcgacac gtatgaggcc
301 gtggtcacag ctgtggatcc cgtggcagac atcgcaacgc tgaggattca gactaaggag
361 cctctcccca cgctgcctct gggacgctca gctgatgtcc ggcaagggga gtttgttgtt
421 gccatgggaa gtccctttgc actgcagaac acgatcacat ccggcattgt tagctctgct
481 cagcgtccag ccagagacct gggactcccc caaaccaatg tggaatacat tcaaactgat
541 gcagctattg attttggaaa cgccggcggt cccctggtta acctggatgg ggaggtgatt
601 ggagtgaaca ccatgaaggt cacagctgga atctcctttg ccatcccttc tgatcgtctt
661 cgagagtttc tgcatcgtgg ggaaaagaag aattcctcct ccggaatcag tgggtcccag
721 cggcgctaca ttggggtgat gatgctgacc ctgagtccca gcatccttgc tgaactacag
781 cttcgagaac caagctttcc cgatgttcag catggtgtac tcatccataa agtcatcctg
841 ggctcccctg cacaccgggc tggtctgcgg cctggtgatg tgattttggc cattggggag
901 cagatggtac aaaatgctga agatgtttat gaagctgttc gaacccaatc ccagttggca
961 gtgcagatcc ggcggggacg agaaacactg accttatatg tgacccctga ggtcacagaa
1021 tgagtcgacg aagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt
1081 gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat
1141 tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat
1201 gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt
1261 tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat
1321 agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct
1381 ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga
1441 ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct gcagcccaag
1501 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
1561 acacaacata cgagccggaa gcataaagtg taaagcctgg gtgcctaatg agtgagctaa
1621 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
1681 cggatccgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
1741 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
1801 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
1861 ttggaggcct aggcttttgc aaaaagctaa cttgtttatt gcagcttata atggttacaa
1921 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
1981 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgctgca ttaatgaatc
2041 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
2101 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
2161 atacggttat ccacagaatc agggataacg caggaaagaa catgtgagca aaaggccagc
2221 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
2281 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
2341 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
2401 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
2461 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
2521 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
2581 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
2641 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
2701 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
2761 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
2821 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
2881 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
2941 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
3001 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
3061 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
3121 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
3181 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
3241 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
3301 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
3361 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
3421 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
3481 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
3541 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
3601 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
3661 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
3721 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
3781 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
3841 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
3901 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
3961 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gggtcgatcg
4021 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
4081 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
4141 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
4201 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
4261 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
4321 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
4381 agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc
4441 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
4501 gggggcgggg gggggggggg ggcccccccc aggcggggcg gggcggggcg aggggcgggg
4561 cggggcgagg cggaaaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc
4621 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg
4681 agtcgttgcg cgctgccttc cccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc
4741 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
4801 gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag
4861 ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt
4921 gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg
4981 cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg
5041 cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg
5101 cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc
5161 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg
5221 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
5281 gccgcctcgg gccggggagg gctcggggga aggggcgcgg cggcccccgg agcgccggcg
5341 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
5401 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
5461 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
5521 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
5581 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
5641 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
5701 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaa
//