LOCUS LT727045 2784 bp DNA circular SYN 06-FEB-2017
DEFINITION Bacterial expression vector pPLc2833, complete sequence.
ACCESSION LT727045
VERSION LT727045.1
KEYWORDS .
SOURCE Bacterial expression vector pPLc2833
ORGANISM Bacterial expression vector pPLc2833
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 2784)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..2784
/organism="Bacterial expression vector pPLc2833"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 483."
/db_xref="taxon:1944697"
rep_origin complement(365..775)
/note="pMB1 ori"
CDS complement(1312..2172)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87284.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 2538..2668
/note="PL"
/regulatory_class="promoter"
BASE COUNT 710 a 712 c 695 g 667 t
ORIGIN
1 ttccggatcc ggctctagag ggtcgaccct ctagagggtc gaccctctag aggctgcagc
61 cgaccccgga tccggccaag cttggctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
121 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
181 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
241 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt
301 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
361 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
421 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
481 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
541 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
601 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
661 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
721 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
781 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
841 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
901 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
961 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
1021 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
1081 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
1141 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
1201 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
1261 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
1321 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
1381 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
1441 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
1501 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
1561 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
1621 cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
1681 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
1741 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
1801 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
1861 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
1921 ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
1981 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
2041 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
2101 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
2161 ttgaatactc atactcttcc tttttcaata ttatgtaagc agacagtttt attgttcatg
2221 atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacaca acgtggcttt
2281 gttgaataaa tcgaactttt gctgagttga aggatcagat cacgcatctt cccgacaacg
2341 cagaccgttc cgtggcaaag caaaagttca aaatcaccaa ctggtccacc tacaacaaag
2401 ctctcatcaa ccgtggctcc ctcactttct ggctggatga tggggcgatt caggcctggt
2461 atgagtcagc aacaccttct tcacgaggca gacctcagcg ccggtgatgc cggccacgat
2521 gcgtccggcg tagaggatct ctcacctacc aaacaatgcc cccctgcaaa aaataaattc
2581 atataaaaaa catacagata accatctgcg gtgataaatt atctctggcg gtgttgacat
2641 aaataccact ggcggtgata ctgagcacat cagcaggacg cactgaccac catgaaggtg
2701 acgctcttaa aattaagccc tgaagaaggg cagcattcaa agcagaaggc tttggggtgt
2761 gtgatacgaa acgaagcatt ggaa
//