LOCUS LT727041 5011 bp DNA circular SYN 06-FEB-2017
DEFINITION Reporter vector pGL3hEcadP-mE2box1, complete sequence.
ACCESSION LT727041
VERSION LT727041.1
KEYWORDS .
SOURCE Reporter vector pGL3hEcadP-mE2box1
ORGANISM Reporter vector pGL3hEcadP-mE2box1
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5011)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5011
/organism="Reporter vector pGL3hEcadP-mE2box1"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4805."
/db_xref="taxon:1945218"
regulatory 7..228
/note="hEcadm"
/regulatory_class="promoter"
CDS 281..1933
/transl_table=11
/note="LUCm"
/protein_id="SJL87277.1"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
regulatory 2021..2117
/note="polyA"
/regulatory_class="terminator"
regulatory complement(2021..2117)
/note="polyA"
/regulatory_class="terminator"
rep_origin complement(2330..2732)
/note="pMB1 ori"
CDS complement(3273..4133)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87278.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
rep_origin 4265..4720
/note="f1 ori"
regulatory complement(4851..5004)
/note="polyA"
/regulatory_class="terminator"
BASE COUNT 1283 a 1213 c 1227 g 1288 t
ORIGIN
1 ggtaccatca gaaccgtgca ggtcccataa cccacctaga ccctagcaac tccaggctag
61 agggtcaacg cgtctatgcg aggccgggtg ggcgggccgt cagctccgcc ctggggaggg
121 gtccgcgctg ctgattggct gtggccggca gatgaaccct cagccaatca gcggtacggg
181 gggcggtgcc tccggggctc acctggctgc agccacgcac cccctctcag tggcgtcgga
241 actgcaagct tggcattccg gtactgttgg taaagccacc atggaagacg ccaaaaacat
301 aaagaaaggc ccggcgccat tctatccgct ggaagatgga accgctggag agcaactgca
361 taaggctatg aagagatacg ccctggttcc tggaacaatt gcttttacag atgcacatat
421 cgaggtggac atcacttacg ctgagtactt cgaaatgtcc gttcggttgg cagaagctat
481 gaaacgatat gggctgaata caaatcacag aatcgtcgta tgcagtgaaa actctcttca
541 attctttatg ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga
601 catttataat gaacgtgaat tgctcaacag tatgggcatt tcgcagccta ccgtggtgtt
661 cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa aaaaagctcc caatcatcca
721 aaaaattatt atcatggatt ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt
781 cgtcacatct catctacctc ccggttttaa tgaatacgat tttgtgccag agtccttcga
841 tagggacaag acaattgcac tgatcatgaa ctcctctgga tctactggtc tgcctaaagg
901 tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg catgccagag atcctatttt
961 tggcaatcaa atcattccgg atactgcgat tttaagtgtt gttccattcc atcacggttt
1021 tggaatgttt actacactcg gatatttgat atgtggattt cgagtcgtct taatgtatag
1081 atttgaagaa gagctgtttc tgaggagcct tcaggattac aagattcaaa gtgcgctgct
1141 ggtgccaacc ctattctcct tcttcgccaa aagcactctg attgacaaat acgatttatc
1201 taatttacac gaaattgctt ctggtggcgc tcccctctct aaggaagtcg gggaagcggt
1261 tgccaagagg ttccatctgc caggtatcag gcaaggatat gggctcactg agactacatc
1321 agctattctg attacacccg agggggatga taaaccgggc gcggtcggta aagttgttcc
1381 attttttgaa gcgaaggttg tggatctgga taccgggaaa acgctgggcg ttaatcaaag
1441 aggcgaactg tgtgtgagag gtcctatgat tatgtccggt tatgtaaaca atccggaagc
1501 gaccaacgcc ttgattgaca aggatggatg gctacattct ggagacatag cttactggga
1561 cgaagacgaa cacttcttca tcgttgaccg cctgaagtct ctgattaagt acaaaggcta
1621 tcaggtggct cccgctgaat tggaatccat cttgctccaa caccccaaca tcttcgacgc
1681 aggtgtcgca ggtcttcccg acgatgacgc cggtgaactt cccgccgccg ttgttgtttt
1741 ggagcacgga aagacgatga cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac
1801 aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac gaagtaccga aaggtcttac
1861 cggaaaactc gacgcaagaa aaatcagaga gatcctcata aaggccaaga agggcggaaa
1921 gatcgccgtg taattctaga gtcggggcgg ccggccgctt cgagcagaca tgataagata
1981 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
2041 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
2101 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
2161 caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccgtcgaccg atgcccttga
2221 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
2281 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctcttccgct
2341 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
2401 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
2461 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
2521 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
2581 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
2641 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
2701 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
2761 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
2821 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
2881 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
2941 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
3001 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
3061 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
3121 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
3181 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
3241 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
3301 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
3361 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
3421 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
3481 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
3541 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
3601 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
3661 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
3721 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
3781 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
3841 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
3901 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
3961 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
4021 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
4081 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
4141 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
4201 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
4261 cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
4321 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
4381 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
4441 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
4501 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
4561 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
4621 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
4681 tttaacgcga attttaacaa aatattaacg tttacaattt gccattcgcc attcaggctg
4741 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gcccaagcta
4801 ccatgataag taagtaatat taaggtacgg gaggtacttg gagcggccgc aataaaatat
4861 ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagta ctaacatacg
4921 ctctccatca aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc cccagtgcaa
4981 gtgcaggtgc cagaacattt ctctatcgat a
//