LOCUS LT727040 5011 bp DNA circular SYN 06-FEB-2017 DEFINITION Reporter vector pGL3hEcadP, complete sequence. ACCESSION LT727040 VERSION LT727040.1 KEYWORDS . SOURCE Reporter vector pGL3hEcadP ORGANISM Reporter vector pGL3hEcadP other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 5011) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..5011 /organism="Reporter vector pGL3hEcadP" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 4804." /db_xref="taxon:1945217" regulatory 7..228 /note="hEcad" /regulatory_class="promoter" CDS 281..1933 /transl_table=11 /note="LUCm" /protein_id="SJL87275.1" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV" regulatory 2021..2117 /note="polyA" /regulatory_class="terminator" regulatory complement(2021..2117) /note="polyA" /regulatory_class="terminator" rep_origin complement(2330..2732) /note="pMB1 ori" CDS complement(3273..4133) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL87276.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 4265..4720 /note="f1 ori" regulatory complement(4851..5004) /note="polyA" /regulatory_class="terminator" BASE COUNT 1282 a 1213 c 1228 g 1288 t ORIGIN 1 ggtaccatca gaaccgtgca ggtcccataa cccacctaga ccctagcaac tccaggctag 61 agggtcaacg cgtctatgcg aggccgggtg ggcgggccgt cagctccgcc ctggggaggg 121 gtccgcgctg ctgattggct gtggccggca ggtgaaccct cagccaatca gcggtacggg 181 gggcggtgcc tccggggctc acctggctgc agccacgcac cccctctcag tggcgtcgga 241 actgcaagct tggcattccg gtactgttgg taaagccacc atggaagacg ccaaaaacat 301 aaagaaaggc ccggcgccat tctatccgct ggaagatgga accgctggag agcaactgca 361 taaggctatg aagagatacg ccctggttcc tggaacaatt gcttttacag atgcacatat 421 cgaggtggac atcacttacg ctgagtactt cgaaatgtcc gttcggttgg cagaagctat 481 gaaacgatat gggctgaata caaatcacag aatcgtcgta tgcagtgaaa actctcttca 541 attctttatg ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga 601 catttataat gaacgtgaat tgctcaacag tatgggcatt tcgcagccta ccgtggtgtt 661 cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa aaaaagctcc caatcatcca 721 aaaaattatt atcatggatt ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt 781 cgtcacatct catctacctc ccggttttaa tgaatacgat tttgtgccag agtccttcga 841 tagggacaag acaattgcac tgatcatgaa ctcctctgga tctactggtc tgcctaaagg 901 tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg catgccagag atcctatttt 961 tggcaatcaa atcattccgg atactgcgat tttaagtgtt gttccattcc atcacggttt 1021 tggaatgttt actacactcg gatatttgat atgtggattt cgagtcgtct taatgtatag 1081 atttgaagaa gagctgtttc tgaggagcct tcaggattac aagattcaaa gtgcgctgct 1141 ggtgccaacc ctattctcct tcttcgccaa aagcactctg attgacaaat acgatttatc 1201 taatttacac gaaattgctt ctggtggcgc tcccctctct aaggaagtcg gggaagcggt 1261 tgccaagagg ttccatctgc caggtatcag gcaaggatat gggctcactg agactacatc 1321 agctattctg attacacccg agggggatga taaaccgggc gcggtcggta aagttgttcc 1381 attttttgaa gcgaaggttg tggatctgga taccgggaaa acgctgggcg ttaatcaaag 1441 aggcgaactg tgtgtgagag gtcctatgat tatgtccggt tatgtaaaca atccggaagc 1501 gaccaacgcc ttgattgaca aggatggatg gctacattct ggagacatag cttactggga 1561 cgaagacgaa cacttcttca tcgttgaccg cctgaagtct ctgattaagt acaaaggcta 1621 tcaggtggct cccgctgaat tggaatccat cttgctccaa caccccaaca tcttcgacgc 1681 aggtgtcgca ggtcttcccg acgatgacgc cggtgaactt cccgccgccg ttgttgtttt 1741 ggagcacgga aagacgatga cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac 1801 aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac gaagtaccga aaggtcttac 1861 cggaaaactc gacgcaagaa aaatcagaga gatcctcata aaggccaaga agggcggaaa 1921 gatcgccgtg taattctaga gtcggggcgg ccggccgctt cgagcagaca tgataagata 1981 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga 2041 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa 2101 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag 2161 caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccgtcgaccg atgcccttga 2221 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac 2281 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctcttccgct 2341 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 2401 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 2461 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 2521 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 2581 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 2641 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 2701 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 2761 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 2821 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 2881 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 2941 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 3001 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 3061 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 3121 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 3181 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 3241 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 3301 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 3361 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 3421 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 3481 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 3541 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 3601 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 3661 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 3721 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 3781 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 3841 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 3901 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 3961 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 4021 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 4081 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 4141 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 4201 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 4261 cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg 4321 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc 4381 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga 4441 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt 4501 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat 4561 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat 4621 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa 4681 tttaacgcga attttaacaa aatattaacg tttacaattt gccattcgcc attcaggctg 4741 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gcccaagcta 4801 ccatgataag taagtaatat taaggtacgg gaggtacttg gagcggccgc aataaaatat 4861 ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagta ctaacatacg 4921 ctctccatca aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc cccagtgcaa 4981 gtgcaggtgc cagaacattt ctctatcgat a //