LOCUS LT727037 5712 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-GFP, complete sequence.
ACCESSION LT727037
VERSION LT727037.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-GFP
ORGANISM Mammalian expression vector pCAGGS-GFP
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5712)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5712
/organism="Mammalian expression vector pCAGGS-GFP"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4800."
/db_xref="taxon:1944867"
gap 14..23
/estimated_length=10
CDS 24..>737
/transl_table=11
/note="GFPm"
/protein_id="SJL87268.1"
/translation="MGKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKD
DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
VLLEFVTAAGITHGMDELYK"
regulatory 1002..1093
/note="beta-globin polyA"
/regulatory_class="terminator"
misc_feature 1094..1450
/note="3' FR of beta-globin"
regulatory complement(1528..1610)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1752..1827
/note="SV40 ori"
regulatory 1858..1932
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(1858..1932)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(2047..2448)
/note="pMB1 ori"
CDS complement(2989..3849)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87269.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3985..4362
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 4368..5709
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4737..5661
/note="intron AG promoter"
BASE COUNT 1300 a 1462 c 1514 g 1422 t
ORIGIN
1 ttcctcgaca tgannnnnnn nnnatgggta aaggagaaga acttttcact ggagttgtcc
61 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg
121 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac
181 tacctgttcc atggccaaca cttgtcacta ctttcacnta tggtgttcaa tgcttttcaa
241 gatacccaga tcatatgaaa cggcatgact ttttcaagag tgccatgccc gaaggttatg
301 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca
361 agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag
421 atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca
481 tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag
541 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg
601 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg
661 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca
721 tggatgaact atacaagtcc ggactcagat ctcgagctca agcttcgaat tctgcagtcg
781 acggtaccgc gggcccggga tccaccggat ctagataact gatcataatc agccatacca
841 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
901 ataaaatgaa tgnnntcgag gaattcactc ctcaggtgca ggctgcctat cagaaggtgg
961 tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca
1021 aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat
1081 ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg
1141 ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg
1201 cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac
1261 agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt
1321 ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt
1381 tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct
1441 tatggagatc cctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct
1501 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
1561 aaagcctggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
1621 ctttccagtc gggaaacctg tcgtgccagc ggatccgcat ctcaattagt cagcaaccat
1681 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
1741 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
1801 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctaac
1861 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
1921 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
1981 catgtctgga tccgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
2041 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
2101 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca gggataacgc
2161 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
2221 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
2281 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
2341 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
2401 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
2461 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
2521 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
2581 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
2641 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
2701 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
2761 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
2821 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
2881 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
2941 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
3001 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
3061 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
3121 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
3181 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
3241 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
3301 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
3361 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
3421 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
3481 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
3541 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
3601 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
3661 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
3721 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
3781 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
3841 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
3901 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
3961 tccccgaaaa gtgccacctg ggtcgacatt gattattgac tagttattaa tagtaatcaa
4021 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
4081 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
4141 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
4201 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
4261 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
4321 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
4381 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
4441 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggccc cccccaggcg
4501 gggcggggcg gggcgagggg cggggcgggg cgaggcggaa aggtgcggcg gcagccaatc
4561 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata
4621 aaaagcgaag cgcgcggcgg gcgggagtcg ttgcgcgctg ccttcccccc gtgccccgct
4681 ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga
4741 gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg
4801 tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg
4861 ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg
4921 cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg
4981 tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga
5041 acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg
5101 tcgggctgca accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg
5161 gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc
5221 aggtgggggt gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaagggg
5281 cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt
5341 ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg
5401 aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc
5461 ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc
5521 cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg
5581 gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat
5641 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat
5701 tttggcaaag aa
//