LOCUS LT727036 6147 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pGal4-p65f1-539, complete sequence.
ACCESSION LT727036
VERSION LT727036.1
KEYWORDS .
SOURCE Mammalian expression vector pGal4-p65f1-539
ORGANISM Mammalian expression vector pGal4-p65f1-539
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6147)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6147
/organism="Mammalian expression vector pGal4-p65f1-539"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4788."
/db_xref="taxon:1945033"
misc_feature 1..825
/note="SV40 VP1 with internal deletion"
regulatory 826..900
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory complement(826..900)
/note="SV40 polyA early"
/regulatory_class="terminator"
CDS complement(921..2540)
/transl_table=11
/note="p65f"
/protein_id="SJL87265.1"
/translation="MDELFPLIFPAEPAQASGPYVEIIEQPKQRGMRFRYKCEGRSAG
SIPGERSTDTTKTHPTIKINGYTGPGTVRISLVTKDPPHRPHPHELVGKDCRDGFYEA
ELCPDRCIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFQVPIEEQRGDYDLNAVRLC
FQVTVRDPSGRPLRLPPVLPHPIFDNRAPNTAELKICRVNRNSGSCLGGDEIFLLCDK
VQKEDIEVYFTGPGWEARGSFSQADVHRQVAIVFRTPPYADPSLQAPVRVSMQLRRPS
DRELSEPMEFQYLPDTDDRHRIEEKRKRTYETFKSIMKKSPFSGPTDPRPPPRRIAVP
SRSSASVPKPAPQPYPFTSSLSTINYDEFPTMVFPSGQISQASALAPAPPQVLPQAPA
PAPAPAMVSALAQAPAPVPVLAPGPPQAVAPPAPKPTQAGEGTLSEALLQLQFDDEDL
GALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQ
RPPDPAPAPLGAPGLPNGLLSGDEDFSSIAD"
misc_feature complement(2541..2576)
/note="link"
CDS complement(<2577..>3014)
/codon_start=1
/transl_table=11
/note="GALbd"
/protein_id="SJL87266.1"
/translation="KLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVN
KDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
regulatory complement(3158..3339)
/note="LTR"
/regulatory_class="promoter"
regulatory 3736..4017
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 3974..4049
/note="SV40 ori"
rep_origin complement(4136..4538)
/note="pMB1 ori"
CDS complement(5079..5939)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87267.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1480 a 1532 c 1612 g 1523 t
ORIGIN
1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg
61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac
121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg
181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc
241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc
301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa
361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg
421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct
481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca
541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc
601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga
661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg
721 tttatgagga cacagaggag cttcctgggg atcgctcgat cgagcgatcc agacatgata
781 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
841 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
901 aattccgcca cggatcaagc ttagtccgca atggaggaga agtcttcatc tcctgaaagg
961 aggccattgg ggagccccgg ggcccccagt ggagcaggag ctgggtcggg gggcctctgg
1021 gcccctgtca ctaggcgagt tatagcctca gggtactcca tcagcatggg ctcagttgtg
1081 tggggggcca caggtatgcc ctggttcagc agctgctgaa actcggagtt gtcgacggat
1141 gccaggtctg tgaacacagc tgggtctgtg ctgttgccaa gcaaggcccc caggtcttca
1201 tcatcaaact gcagctgcag cagggcctct gacagcgttc cttccccagc ctgggtgggc
1261 ttgggggcag gtggggccac agcctgagga gggcctgggg ctaggactgg gacaggggct
1321 ggggcctggg ccagagctga taccatggct ggagcagggg caggggctgg agcctggggc
1381 aggacttggg gaggggccgg ggccaaggcc gaggcctggc tgatctgccc agaaggaaac
1441 accatggtgg gaaactcatc atagttgatg gtgctcaggg atgacgtaaa gggatagggc
1501 tggggtgctg gcttggggac agaagctgag ctgcgggaag gcacagcaat gcgtcgaggt
1561 ggaggccggg ggtcggtggg tccgctgaaa ggactcttct tcatgatgct cttgaaggtc
1621 tcatatgtcc ttttacgttt ctcctcaatc cggtgacgat cgtctgtatc tggcaggtac
1681 tggaattcca tgggctcact gagctcccgg tcggaaggcc gccgcagctg catggagaca
1741 cgcacaggag cctgcaggct ggggtctgcg tagggagggg tccggaacac aatggccact
1801 tgtcggtgca catcagcttg cgaaaaggag cctcgggcct cccagcctgg tcccgtgaaa
1861 tacacctcaa tgtcctcttt ctgcaccttg tcacacagta ggaagatctc atccccaccg
1921 aggcagctgc cagagtttcg gttcactcgg cagatcttga gctcggcagt gttgggggca
1981 cgattgtcaa agatgggatg aggaaggaca ggcggcaggc ggaggggcct gcctgatggg
2041 tcccgcactg tcacctggaa gcagagccgc acagcattca ggtcgtagtc cccacgctgc
2101 tcttctatag gaacttggaa ggggttgttg ttggtctgga tgcgctgact gatagcctgc
2161 tccaggtccc gcttcttcac acactggatt cccaggttct ggaaactgtg gatgcagcgg
2221 tccgggcaga gctcagcctc atagaagcca tcccggcagt cctttcctac aagctcgtgg
2281 gggtgaggcc ggtgaggagg gtccttggtg accagggaga tgcgcactgt ccctggtcct
2341 gtgtagccat tgatcttgat ggtggggtgg gtcttggtgg tatctgtgct cctctcgcct
2401 gggatgctgc ccgcggagcg cccctcgcac ttgtagcgga agcgcatgcc ccgctgcttg
2461 ggctgctcaa tgatctccac ataggggcca gaggcctggg ctggctctgc cgggaagatg
2521 agggggaaca gttcgtccat ggccggggtc ccctggtcga cacccgggaa ttccggcgat
2581 acagtcaact gtctttgacc tttgttacta ctctcttccg atgatgatgt cgcacttatt
2641 ctatgctgtc tcaatgttag aggcatatca gtctccactg aagccaatct atctgtgacg
2701 gcatctttat tcacattatc ttgtacaaat aatcctgtta acaatgcttt tatatcctgt
2761 aaagaatcca ttttcaaaat catgtcaagg tcttctcgag gaaaaatcag tagaaatagc
2821 tgttccagtc tttctagcct tgattccact tctgtcagat gtgccctagt cagcggagac
2881 cttttggttt tgggagagta gcgacactcc cagttgttct tcagacactt ggcgcacttc
2941 ggtttttctt tggagcactt gagcttttta agtcggcaaa tatcgcatgc ttgttcgata
3001 gaagacagta gcttcagatc tggctgctgg gagtccatca gtgaatatca actctggcag
3061 aggagccgct cgcccgccac cgtccgcagt tcccgccgca gccgagataa acaacttagc
3121 ttgtgaacgc agaaggagca ggaggtaccc agcttggagg tgcacaccaa tgtggtgaat
3181 ggtcaaatgg cgtttattgt atcgagctag gcacttaaat acaatatctc tgcaatgcgg
3241 aattcagtgg ttcgtccaat ccatgtcaga cctgtctgtt gccttcctaa taaggcacga
3301 tcgtaccacc ttacttccac caatcggcat gcacggtgct ttttctctcc ttgtaaggca
3361 tgttgctaac tcatcgttac catgttgcaa gactacaagt gtattgcata agactacatt
3421 tccccctccc tatgcaaaag cgaaactact atatcctgag gggactccta accgcgtaca
3481 accgaagccc cgcttttcgc ctaaacacac cctagtcccc tcagatacgc gtatatctgg
3541 cccgtacatc gcgaagcagc gcaaaacgcc taaccctaag cagattcttc atgcaattgt
3601 cggtcaagcc ttgccttgtt gtagcttaaa ttttgctcgc gcactactca gcgacctcca
3661 acacacaagc agggagcaga tactggctta actatgcggc atcagagcag attgtactga
3721 gagtgcacca tatggctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
3781 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
3841 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
3901 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
3961 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
4021 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
4081 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct
4141 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
4201 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
4261 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
4321 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
4381 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
4441 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
4501 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
4561 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
4621 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
4681 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
4741 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
4801 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
4861 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
4921 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
4981 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
5041 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
5101 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
5161 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
5221 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
5281 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
5341 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc
5401 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
5461 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
5521 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
5581 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
5641 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg
5701 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
5761 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
5821 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
5881 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
5941 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
6001 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
6061 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
6121 atcacgaggc cctttcgtct tcaagaa
//