LOCUS LT727034 5339 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-E-mBNIP3, complete sequence.
ACCESSION LT727034
VERSION LT727034.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-E-mBNIP3
ORGANISM Mammalian expression vector pCAGGS-E-mBNIP3
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5339)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5339
/organism="Mammalian expression vector pCAGGS-E-mBNIP3"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4773."
/db_xref="taxon:1944805"
misc_feature 13..51
/note="E-tag"
CDS 64..627
/transl_table=11
/note="mBNIP3"
/protein_id="SJL87256.1"
/translation="MSQSGEENLQGSWVELHFSNGNGSSVPASVSIYNGDMEKILLDA
QHESGRSSSKSSHCDSPPRSQTPQDTNRAEIDSHSFGEKNSTLSEEDYIERRREVESI
LKKNSDWIWDWSSRPENIPPKEFLFKHPKRTATLSMRNTSVMKKGGIFSADFLKVFLP
SLLLSHLLAIGLGIYIGRRLTTSTSTF"
regulatory 629..720
/note="beta-globin polyA"
/regulatory_class="terminator"
misc_feature 721..1077
/note="3' FR of beta-globin"
regulatory complement(1155..1237)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1379..1454
/note="SV40 ori"
regulatory 1485..1559
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(1485..1559)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(1674..2075)
/note="pMB1 ori"
CDS complement(2616..3476)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87257.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3612..3989
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 3995..5336
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4364..5288
/note="intron AG promoter"
BASE COUNT 1161 a 1398 c 1469 g 1311 t
ORIGIN
1 ttcctcgaga tgggtgcgcc ggtgccgtat ccggatccgc tggaaccgcg tgaattcggt
61 accatgtcgc agagcgggga ggagaacctg cagggctcct gggtagaact gcacttcagc
121 aatggcaatg ggagcagcgt tccagcctcc gtctctattt ataatggtga catggaaaaa
181 atactgttgg atgcccagca tgaatctgga cgaagtagct ccaagagttc tcactgtgac
241 agcccacctc gctcccagac accacaagat accaacagag ctgaaataga cagccacagc
301 tttggcgaga aaaacagcac tctgtctgag gaagattata ttgagagaag aagagaagtt
361 gaaagtatcc tgaagaaaaa ctcagattgg atatgggatt ggtcaagtcg accagaaaat
421 attcccccca aggagttcct ttttaaacac ccgaagcgca cagctactct cagcatgaga
481 aacacaagcg ttatgaagaa agggggaatt ttctcagcag actttctgaa ggttttcctt
541 ccatctctgt tactgtctca tctgctggcc attggcttgg ggatctacat tggaaggcgt
601 ctgacaactt ccactagcac cttctgaaga tctttttccc tctgccaaaa attatgggga
661 catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc
721 aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca
781 tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc
841 tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt
901 ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt
961 tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac tagccagatt
1021 tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct
1081 cgacctgcag cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
1141 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctgggtgc
1201 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
1261 aaacctgtcg tgccagcgga tccgcatctc aattagtcag caaccatagt cccgccccta
1321 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
1381 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
1441 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctaacttg tttattgcag
1501 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
1561 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc
1621 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
1681 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
1741 tcactcaaag gcggtaatac ggttatccac agaatcaggg ataacgcagg aaagaacatg
1801 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
1861 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
1921 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
1981 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
2041 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
2101 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
2161 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
2221 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
2281 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
2341 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
2401 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
2461 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
2521 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
2581 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
2641 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
2701 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
2761 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
2821 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
2881 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
2941 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
3001 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
3061 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
3121 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
3181 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
3241 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
3301 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
3361 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
3421 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
3481 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
3541 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
3601 ccacctgggt cgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
3661 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
3721 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
3781 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
3841 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
3901 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
3961 catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac
4021 tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt
4081 ttgtgcagcg atgggggcgg gggggggggg ggggcccccc ccaggcgggg cggggcgggg
4141 cgaggggcgg ggcggggcga ggcggaaagg tgcggcggca gccaatcaga gcggcgcgct
4201 ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc
4261 gcggcgggcg ggagtcgttg cgcgctgcct tccccccgtg ccccgctccg ccgccgcctc
4321 gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg
4381 cccttctcct ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg
4441 gctgcgtgaa agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg
4501 gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg
4561 ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag
4621 cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt
4681 gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc
4741 ccccctgcac ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg
4801 tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc
4861 gggcggggcg gggccgcctc gggccgggga gggctcgggg gaaggggcgc ggcggccccc
4921 ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt
4981 gcgagagggc gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc
5041 gccgccgcac cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa
5101 tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc
5161 ggggctgtcc gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct
5221 tctggcgtgt gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt
5281 tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaa
//