LOCUS LT727032 8781 bp DNA circular SYN 06-FEB-2017 DEFINITION Yeast expression vector pAS2.1htBID, complete sequence. ACCESSION LT727032 VERSION LT727032.1 KEYWORDS . SOURCE Yeast expression vector pAS2.1htBID ORGANISM Yeast expression vector pAS2.1htBID other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 8781) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..8781 /organism="Yeast expression vector pAS2.1htBID" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 4759." /db_xref="taxon:1945444" rep_origin complement(2..982) /note="2mu ori" regulatory complement(1060..1164) /note="FLP terminator" /regulatory_class="terminator" CDS 1884..2558 /transl_table=11 /note="TRP1" /protein_id="SJL87247.1" /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF VKNAKK" rep_origin complement(2661..3116) /note="f1 ori" regulatory complement(3312..3586) /note="CYC1 terminator" /regulatory_class="terminator" CDS complement(3855..4304) /transl_table=11 /note="CYH2" /protein_id="SJL87248.1" /translation="MPSRFTKTRKHRGHVSAGKGRIGKHRKHPGGRGMAGGQHHHRIN MDKYHPGYFGKVGMRYFHKQQAHFWKPVLNLDKLWTLIPEDKRDQYLKSASKETAPVI DTLAAGYGKILGKGRIPNVPVIVKARFVSKLAEEKIRAAGGVVELIA" regulatory 5075..5478 /note="ADH1 promoter" /regulatory_class="promoter" CDS 5501..>5941 /transl_table=11 /note="GALbd" /protein_id="SJL87249.1" /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS" CDS <5984..6391 /codon_start=1 /transl_table=11 /note="htBID" /protein_id="SJL87250.1" /translation="GNRSSHSRLGRIEADSESQEDIIRNIARHLAQVGDSMDRSIPPG LVNGLALQLRNTSRSEEDRNRDLATALEQLLQAYPRDMEKEKTMLVLALLLAKKVASH TPSLLRDVFHTTVNFINQNLRTYVRSLARNGMD" regulatory 6419..6520 /note="ADH1 terminator" /regulatory_class="terminator" regulatory complement(6673..6756) /note="lac promoter" /regulatory_class="promoter" rep_origin complement(6848..7250) /note="pMB1 ori" CDS complement(7791..8651) /transl_table=11 /note="ampicillin" /protein_id="SJL87251.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" BASE COUNT 2448 a 1866 c 1986 g 2481 t ORIGIN 1 gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt 61 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt 121 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta 181 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg 241 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag 301 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat 361 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg 421 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact 481 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga 541 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca 601 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac 661 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa 721 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag 781 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag 841 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt 901 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg 961 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt 1021 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca 1081 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt 1141 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc 1201 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact 1261 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc 1321 tatcatttcc tttgatattg gatcatatta agaaaccatt attatcatga cattaaccta 1381 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 1441 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 1501 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 1561 tgcggcatca gagcagattg tactgagagt gcaccataga tcaacgacat tactatatat 1621 ataatatagg aagcatttaa tagacagcat cgtaatatat gtgtactttg cagttatgac 1681 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca 1741 atcttgatcc ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa 1801 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta 1861 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat 1921 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg 1981 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg 2041 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact 2101 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg 2161 gcattgatat cgtccaactg catggagatg agtcgtggca agaataccaa gagttcctcg 2221 gtttgccagt tattaaaaga ctcgtatttc caaaagactg caacatacta ctcagtgcag 2281 cttcacagaa acctcattcg tttattccct tgtttgattc agaagcaggt gggacaggtg 2341 aacttttgga ttggaactcg atttctgact gggttggaag gcaagagagc cccgaaagct 2401 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa 2461 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta 2521 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat 2581 ttaagtattg tttgtgcact tgccgatcta tgcggtgtga aataccgcac agatgcgtaa 2641 ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa 2701 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa 2761 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact 2821 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc 2881 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa 2941 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc 3001 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt 3061 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg 3121 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 3181 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 3241 gttttcccag tcacgacgtt gtaaaacgac ggccagtcgt ccaagctttc gcgagctcga 3301 gatcccgagc tttgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt 3361 tcagtataat gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata 3421 taaataacgt tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag 3481 gttgtctaac tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc 3541 gtgacataac taattacatg atatcgacaa aggaaaaggg gcctgtttac tcacaggctt 3601 ttttcaagta ggtaattaag tcgtttctgt ctttttcctt cttcaaccca ccaaaggcca 3661 tcttggtact tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt 3721 tttttttttt tttttttttt tttttttttt tttttttttt tttcatagaa ataatacaga 3781 agtagatgtt gaattagatt aaactgaaga tatataattt attggaaaat acatagagct 3841 ttttgttgat gcgcttaagc gatcaattca acaacaccac cagcagctct gattttttct 3901 tcagccaact tggagacgaa tctagctttg acgataactg gaacatttgg gattctaccc 3961 ttacccaaga tcttaccgta accggctgcc aaagtgtcaa taactggagc agtttcctta 4021 gaagcagatt tcaagtattg gtctctcttg tcttctggga tcaatgtcca caatttgtcc 4081 aagttcaaga ctggcttcca gaaatgagct tgttgcttgt ggaagtatct cataccaacc 4141 ttaccgaaat aacctggatg gtatttatcc atgttaattc tgtggtgatg ttgaccaccg 4201 gccatacctc taccaccggg gtgctttctg tgcttaccga tacgaccttt accggctgag 4261 acgtgacctc tgtgctttct agtcttagtg aatctggaag gcattcttga ttagttggat 4321 gattgttctg ggatttaatg caaaaaaatc actaagaagg aaaaaaatca acggagaaag 4381 caaacgccat cttaaatata cgggatacag atgaaaggtt tgaacctatc tgggaaaata 4441 cgcattaaac aagcgaaaaa ctgcgaggaa aattgtttgc gtctctgcgg gctattcacg 4501 cgccagagga aaataggaaa aataacaggg cattagaaaa ataattttga ttttggtaat 4561 gtgtgggtcc ctggtgtaca gatgttacat tggttacagt actcttgttt ttgctgtgtt 4621 tttcgatgaa tctccaaaat ggttgttagc acatggaaga gtcaccgatg ctaagttatc 4681 tctatgtaag ctacgtggcg tgacttttga tgaagccgca caagagatac aggattggca 4741 actgcaaata gaatctgggg atctagatat ccttttgttg tttccgggtg tacaatatgg 4801 acttcctctt ttctggcaac caaacccata catcgggatt cctataatac cttcgttggt 4861 ctccctaaca tgtaggtggc ggaggggaga tatacaatag aacagatacc agacaagaca 4921 taatgggcta aacaagacta caccaattac actgcctcat tgatggtggt acataacgaa 4981 ctaatactgt agccctagac ttgatagcca tcatcatatc gaagtttcac tacccttttt 5041 ccatttgcca tctattgaag taataatagg cgcatgcaac ttcttttctt tttttttctt 5101 ttctctctcc cccgttgttg tctcaccata tccgcaatga caaaaaaaat gatggaagac 5161 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga 5221 tgaggggtat cttcgaacac acgaaacttt ttccttcctt cattcacgca cactactctc 5281 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaagttt 5341 gccgctttgc tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat 5401 tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag 5461 catacaatca actccaagct tgaagcaagc ctcctgaaag atgaagctac tgtcttctat 5521 cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa 5581 gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc 5641 tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct 5701 atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca 5761 ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt 5821 cacagataga ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat 5881 aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc 5941 gccggtattg caatacccag ctttgactca tatggccatg gcgggcaacc gcagcagcca 6001 ctcccgcttg ggaagaatag aggcagattc tgaaagtcaa gaagacatca tccggaatat 6061 tgccaggcac ctcgcccagg tcggggacag catggaccgt agcatccctc cgggcctggt 6121 gaacggcctg gccctgcagc tcaggaacac cagccggtcg gaggaggacc ggaacaggga 6181 cctggccact gccctggagc agctgctgca ggcctaccct agagacatgg agaaggagaa 6241 gaccatgctg gtgctggccc tgctgctggc caagaaggtg gccagtcaca cgccgtcctt 6301 gctccgtgat gtctttcaca caacagtgaa ttttattaac cagaacctac gcacctacgt 6361 gaggagctta gccagaaatg ggatggactg agtcgacctg cagccaagct aattccgggc 6421 gaatttctta tgatttatga tttttattat taaataagtt ataaaaaaaa taagtgtata 6481 caaattttaa agtgactctt aggttttaaa acgaaaattc ttgttcttga gtaactcttt 6541 cctgtaggtc aggttgcttt ctcaggtata gcatgaggtc gctcttattg accacacctc 6601 taccggcatg caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat 6661 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc 6721 taatgagtga ggtaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 6781 aacctgtcgt gccagctgga ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 6841 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 6901 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 6961 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 7021 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 7081 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 7141 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 7201 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 7261 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 7321 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 7381 gcagccacta gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 7441 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg 7501 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 7561 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 7621 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 7681 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 7741 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 7801 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 7861 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 7921 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 7981 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 8041 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 8101 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 8161 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 8221 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 8281 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 8341 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 8401 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 8461 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 8521 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 8581 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 8641 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 8701 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 8761 tttccccgaa aagtgccacc t //