LOCUS LT727027 5085 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-E-mABIN-1(469-560), complete
sequence.
ACCESSION LT727027
VERSION LT727027.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-E-mABIN-1(469-560)
ORGANISM Mammalian expression vector pCAGGS-E-mABIN-1(469-560)
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5085)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5085
/organism="Mammalian expression vector
pCAGGS-E-mABIN-1(469-560)"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5373."
/db_xref="taxon:1944799"
misc_feature 16..54
/note="E-tag"
CDS 64..342
/transl_table=11
/note="mABIN-1(469-560)"
/protein_id="SJL87227.1"
/translation="MTQNELLKQQVKIFEEDFQRERSDRERMNEEKEELKKQVEKLQA
QVTLTNAQLKTLKEEEKAKEALKQQKRKAKASGERYHMEPHPEHVCGA"
regulatory 375..466
/note="beta-globin polyA"
/regulatory_class="terminator"
misc_feature 467..823
/note="3' FR of beta-globin"
regulatory complement(901..983)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1125..1200
/note="SV40 ori"
regulatory complement(1231..1305)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 1231..1305
/note="SV40 polyA early"
/regulatory_class="terminator"
rep_origin complement(1420..1821)
/note="pMB1 ori"
CDS complement(2362..3222)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87228.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3358..3735
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 3741..5082
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4110..5034
/note="intron AG promoter"
BASE COUNT 1097 a 1347 c 1422 g 1219 t
ORIGIN
1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
61 gcagtgacac agaatgagtt gctgaaacag caggtaaaga tctttgaaga ggacttccag
121 agggaacgga gtgaccgtga acgcatgaat gaagagaagg aggagctgaa gaagcaagta
181 gagaagctgc aggcccaggt caccctgact aatgcccagc tcaaaactct caaagaggag
241 gagaaggcca aggaagccct caaacagcag aagaggaaag caaaggcttc gggagagcgc
301 taccacatgg aaccccaccc tgagcacgtc tgcggcgcct gacccccaat gccctggctc
361 acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc
421 ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga
481 attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga
541 atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag
601 gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc
661 atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt
721 ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct
781 gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac ctgcagccca
841 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
901 ccacacaaca tacgagccgg aagcataaag tgtaaagcct gggtgcctaa tgagtgagct
961 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
1021 agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
1081 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
1141 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
1201 ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta taatggttac
1261 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
1321 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgctg cattaatgaa
1381 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
1441 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
1501 taatacggtt atccacagaa tcagggataa cgcaggaaag aacatgtgag caaaaggcca
1561 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
1621 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
1681 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
1741 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
1801 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
1861 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
1921 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
1981 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
2041 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
2101 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
2161 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
2221 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
2281 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
2341 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
2401 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
2461 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
2521 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
2581 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
2641 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
2701 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
2761 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
2821 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
2881 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
2941 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
3001 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
3061 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
3121 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
3181 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
3241 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
3301 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgggtcgac
3361 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
3421 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
3481 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
3541 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
3601 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
3661 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
3721 tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc
3781 ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg
3841 gggcgggggg gggggggggg ccccccccag gcggggcggg gcggggcgag gggcggggcg
3901 gggcgaggcg gaaaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt
3961 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag
4021 tcgttgcgcg ctgccttccc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc
4081 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg
4141 gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc
4201 ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt
4261 gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg
4321 ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg
4381 gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
4441 tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc
4501 ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg
4561 cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc
4621 cgcctcgggc cggggagggc tcgggggaag gggcgcggcg gcccccggag cgccggcggc
4681 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
4741 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
4801 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
4861 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
4921 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
4981 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
5041 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaa
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