LOCUS LT727025 5283 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-E-mABIN-1(444-601), complete
sequence.
ACCESSION LT727025
VERSION LT727025.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-E-mABIN-1(444-601)
ORGANISM Mammalian expression vector pCAGGS-E-mABIN-1(444-601)
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5283)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5283
/organism="Mammalian expression vector
pCAGGS-E-mABIN-1(444-601)"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5371."
/db_xref="taxon:1944796"
misc_feature 16..54
/note="E-tag"
CDS <64..540
/codon_start=1
/transl_table=11
/note="mABIN-1(444-601)"
/protein_id="SJL87223.1"
/translation="ASPSSPPAAFGSPEGVGGHLRKQELVTQNELLKQQVKIFEEDFQ
RERSDRERMNEEKEELKKQVEKLQAQVTLTNAQLKTLKEEEKAKEALKQQKRKAKASG
ERYHMEPHPEHVCGAYPYAYPPMPAMVPHHAYKDWSQIRYPPPPVPMEHPPPHPNS"
regulatory 573..664
/note="beta-globin polyA"
/regulatory_class="terminator"
misc_feature 665..1021
/note="3' FR of beta-globin"
regulatory complement(1099..1181)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1323..1398
/note="SV40 ori"
regulatory complement(1429..1503)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 1429..1503
/note="SV40 polyA early"
/regulatory_class="terminator"
rep_origin complement(1618..2019)
/note="pMB1 ori"
CDS complement(2560..3420)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87224.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3556..3933
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 3939..5280
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4308..5232
/note="intron AG promoter"
BASE COUNT 1138 a 1426 c 1463 g 1256 t
ORIGIN
1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
61 gcagcctctc catcatctcc gcctgcagct tttgggagtc cagaaggcgt tgggggccat
121 ctgaggaagc aggaactagt gacacagaat gagttgctga aacagcaggt aaagatcttt
181 gaagaggact tccagaggga acggagtgac cgtgaacgca tgaatgaaga gaaggaggag
241 ctgaagaagc aagtagagaa gctgcaggcc caggtcaccc tgactaatgc ccagctcaaa
301 actctcaaag aggaggagaa ggccaaggaa gccctcaaac agcagaagag gaaagcaaag
361 gcttcgggag agcgctacca catggaaccc caccctgagc acgtctgcgg cgcctatccc
421 tatgcctacc cacccatgcc agccatggta cctcaccatg cctacaagga ctggtcccag
481 atccgatacc ctccaccccc tgtgcccatg gagcacccgc ccccacaccc caactcttga
541 cccccaatgc cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg
601 gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca
661 ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa
721 atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc
781 tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg
841 ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt
901 gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca
961 gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat
1021 ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
1081 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
1141 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
1201 cgggaaacct gtcgtgccag cggatccgca tctcaattag tcagcaacca tagtcccgcc
1261 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
1321 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
1381 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctaa cttgtttatt
1441 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
1501 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
1561 atccgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
1621 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
1681 cagctcactc aaaggcggta atacggttat ccacagaatc agggataacg caggaaagaa
1741 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
1801 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
1861 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
1921 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
1981 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
2041 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
2101 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
2161 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
2221 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
2281 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
2341 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
2401 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
2461 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
2521 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
2581 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
2641 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
2701 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
2761 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
2821 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
2881 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
2941 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
3001 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
3061 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
3121 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
3181 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
3241 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
3301 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
3361 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
3421 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
3481 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
3541 agtgccacct gggtcgacat tgattattga ctagttatta atagtaatca attacggggt
3601 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
3661 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
3721 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
3781 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
3841 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
3901 agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct
3961 tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt attttttaat
4021 tattttgtgc agcgatgggg gcgggggggg ggggggggcc ccccccaggc ggggcggggc
4081 ggggcgaggg gcggggcggg gcgaggcgga aaggtgcggc ggcagccaat cagagcggcg
4141 cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa
4201 gcgcgcggcg ggcgggagtc gttgcgcgct gccttccccc cgtgccccgc tccgccgccg
4261 cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg
4321 acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc
4381 tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct
4441 cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg
4501 gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg
4561 ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct
4621 gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc
4681 aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc
4741 tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg
4801 tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggaaggg gcgcggcggc
4861 ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa
4921 tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg
4981 aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag
5041 gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag
5101 cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc
5161 ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc
5221 tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa
5281 gaa
//